High Pressure Freezing of Rust Infected Plant Leaves

Mendgen, Kurt; Welter, Klaus; Scheffold, Frank; Knauf-Beiter, G.

doi:10.1007/978-3-642-75818-8_3

Cited by 46 publications

(26 citation statements)

References 30 publications

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“…Twenty-four hours after inoculation roots were infiltrated with 8% (v/v) methanol and immediately placed into 2-mm-diameter, 0.2-mm-deep aluminium cups. Within 5 min after infiltration, samples were frozen in a Balzers HPM 10 (Balzers Union Liechtenstein) high-pressure-freezing instrument (Mendgen et al 1991). Samples were freeze-substituted with 2% (w/v) osmium tetroxide in acetone in three steps: 24 h at 90 ~ 12 h at -60 ~ and 9 h at -30 ~ After substitution, the temperature was raised to 0 ~ the samples were rinsed three times for 15 min with acetone and infiltrated with epon-araldite epoxide resin.…”

Section: Methodsmentioning

confidence: 99%

Cell wall synthesis in cotton roots after infection with Fusarium oxysporum

Rodríguez-Gálvez

Mendgen

1995

Planta

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Abstract. Fusarium oxysporum f. sp. vasinfectum penetration hyphae infect living cells in the meristematic zone of cotton (Gossypium barbadense L.) roots. We characterized wall modifications induced by the fungus during infection of the protodermis using antibodies against callose, arabinogalactan-proteins, xyloglucan, pectin, polygalacturonic acid and rhamnogalacturonan I in high-pressure frozen, freeze-substituted root tissue. Using quantitative immunogold labelling we compared the cell walls before and after hyphal contact, cell plates with plasmodesmata during cytokinesis, and wall appositions induced by fungal contact. In the already-existing wall, fungal contact induced only minor modifications such as an increase of xyloglucan epitopes. Wall appositions mostly exhibited epitopes similar to the cell plate except that wall appositions had a much higher callose content. This study shows that wall appositions induced by Fusarium oxysporum hyphae are the result of normal cell wall synthesis and the addition of large amounts of callose. The appositions do not stop fungal growth.

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Section: Methodsmentioning

confidence: 99%

Cell wall synthesis in cotton roots after infection with Fusarium oxysporum

Rodríguez-Gálvez

Mendgen

1995

Planta

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“…Cells on polycarbonate or cellophane membranes were cryo-fixed by plunging into liquid propane [21] or by propane jet freezing [17]. For infected tissues, it was necessary to use high-pressure freezing, since this is the only method currently available for cryo-fixation of thick specimens (up to 0.5 mm) without the fonnation of damaging ice crystals [33]. After freeze-substitution in osmium-acetone, samples were embedded at -20°C in LR White resin [53].…”

Section: Localisation Of Antigensmentioning

confidence: 99%

Use of Monoclonal Antibodies to Study Differentiation of Colletotrichum Infection Structures

O’Connell

Pain

Bailey

et al. 1996

Developments in Plant Pathology

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“…2 cm • 2 cm • 0.3 cm) were infiltrated with 10% methanol in distilled water under vacuum (20 mm Hg) for 2 min to remove air from intercellular compartments Meikle et al 1991 m Monoclonal, p polyclonal (Mendgen et al 1991). Pieces of leaf tissue were excised using a 2 mm corkborer, then mounted in hexadecene between two aluminium holders (0.3 mm deep) and cryofixed using a Balzers HPM 010 high pressure freezing apparatus.…”

Section: Preparation Of Plant Materials For Electron Microscopymentioning

confidence: 99%

Sequential deposition of plant glycoproteins and polysaccharides at the host-parasite interface ofUromyces vignae andVigna sinensis

Stark-Urnau

Mendgen

1995

Protoplasma

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Summary. Monokaryotic haustoria (M-haustoria) of Uromyces vig-nae in Vigna sinensis cells are surrounded by an extrahaustorial matrix (ema) and the invaginated host plasmalemma, the extrahaustorial membrane (ehm). The ema was characterized with antibodies against components of the plant cell wall; the ema contained hydroxyproline-rich glycoproteins and arabinogalactans/arabinogalactan proteins, both at a higher concentration close to the ehm. Haustoria with large vacuoles had the ema encased by additional layers. An electron-translucent inner layer deposited on top of the ema contained arabinogalactans/arabinogalactan proteins, hydroxyprolinerich glycoproteins, and callose. The inner layer was surrounded by an electron-translucent middle layer with numerous dark inclusions, rich in pectin and fucose bound to xyloglucans. Finally, a more electron-dense outer layer containing arabinogalactans/arabinogalactan proteins and hydroxyproline-rich glycoproteins encased the whole structure. These polysaccharides, with the exception of callose and un-esterified pectin, 'were also found in the plant Golgi apparatus. The polysaccharides were synthesized in the trans Golgi cisternae and secreted into the host-parasite interface. The secretory events seem to be coupled to endocytosis since numerous coated pits were found on the ehm too. The pits were elongated, sometimes formed tubules and the coat reacted with an antibody against plant clathrin. Our results suggest intensive membrane recycling around haustoria, together with the secretion of cell wall material, which in the case of more or less vacuolated haustoria seems to be responsible for encasement

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High Pressure Freezing of Rust Infected Plant Leaves

Cited by 46 publications

References 30 publications

Cell wall synthesis in cotton roots after infection with Fusarium oxysporum

Cell wall synthesis in cotton roots after infection with Fusarium oxysporum

Use of Monoclonal Antibodies to Study Differentiation of Colletotrichum Infection Structures

Sequential deposition of plant glycoproteins and polysaccharides at the host-parasite interface ofUromyces vignae andVigna sinensis

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