High-Pressure Extraction of Antioxidant-Rich Fractions from Shrubby Cinquefoil (Dasiphora fruticosa L. Rydb.) Leaves: Process Optimization and Extract Characterization

Abstract

Dasiphora fruticosa (basionym Potentilla fruticosa) is a shrub, known in traditional medicine for centuries. Due to the wide range of pharmacological effects, interest and applications of D. fruticosa extracts are continually increasing; however, reports on optimization of extraction conditions are scarce. Herein, a multi-step high-pressure extraction process with increasing polarity solvents was developed to isolate valuable fractions from D. fruticosa leaves. Supercritical CO2 extraction recovered 2.46 g/100 g of lipophilic fraction, rich in polyunsaturated fatty acids. Further, pressurized liquid extractions (PLE) with acetone, ethanol, and water were applied to obtain antioxidant-rich higher polarity extracts. Under optimized PLE conditions, the cumulative polar fraction yield was 29.98 g/100 g. Ethanol fraction showed the highest yield (15.3 g/100 g), TPC values (148.4 mg GAE/g), ABTS•+, and DPPH• scavenging capacity (161.1 and 151.8 mg TE/g, respectively). PLE was more efficient than conventional solid–liquid extraction in terms of extraction time, extract yields, and in vitro antioxidant capacity. Phytochemical characterization of PLE extracts by UPLC-Q-TOF-MS revealed the presence of hyperoside, ellagic acid, among other health beneficial phenolic substances. Τhis study highlights the potential of high-pressure extraction techniques to isolate antioxidant-rich fractions from D. fruticosa leaves with multipurpose applications, including the prevention and treatment of chronic diseases.

Application of Raw and Defatted by Supercritical CO2 Hemp Seed Press-Cake and Sweet Grass Antioxidant Extract in Pork Burger Patties

“…Dried sweet grass (Hierochloe odorata) was purchased from the company Mėta (Vaidotai, Vilnius, Lithuania). Previously optimised for herbal materials, high-pressure extraction procedures were applied to sweet grass with slight modifications [15]. First, the herb was ground and extracted in a pilot-scale extractor with supercritical CO 2 at 40 MPa and 60 • C for removing lipophilic substances and volatile aroma constituents (sweet grass possesses a strong specific aroma of coumarin).…”

Optimized Supercritical CO2 Extraction Enhances the Recovery of Valuable Lipophilic Antioxidants and Other Constituents from Dual-Purpose Hop (Humulus lupulus L.) Variety Ella

“…CCD-RSM was employed to identify optimal SFE-CO 2 conditions by determining the effect of P (25-45 MPa), T (40-60 • C) and τ (30-90 min) on SFE-CO 2 extract yield and ORAC, selected as the response factors (RF) in the optimization experiments. The face-centered CCD design with 8 factorial, 6 axial and 6 center points (in total, 20 experimental runs), randomized order of experiments, models and the second-order polynomial equations for both RFs were established using the Design-Expert 12 software (Stat-Ease Inc., Minneapolis, MN) as previously described elsewhere by our research group [15][16][17][18][19]. Student test (pvalue) at 5% probability level (p < 0.05), "lack of fit" coefficient and the Fisher test value (F-value) were used to define the statistical significance and adequacy of the model and each variable for both RFs.…”

“…The so-called dual-purpose hop varieties, which are rich in essential oil and bitter acids [6], such as Ella, Citrus, Columbus, Galaxy and others, can serve as a promising feedstock for these purposes. Given our group's interest in the utilization of emerging techniques to recover high added-value constituents from various feedstocks and agroindustrial residues [15][16][17][18][19], the present work is aimed at developing an effective SFE-CO 2 process for production of natural dual-purpose hop extracts from Ella (previously named Stella) variety with high yield and strong oxygen radical scavenging capacity (ORAC). Towards this end, critical SFE-CO 2 conditions, such as temperature, pressure and time were optimized via the response surface methodology (RSM) using central composite design (CCD) [20].…”

Antioxidant Capacity of Potentilla paradoxa Nutt. and Its Beneficial Effects Related to Anti-Aging in HaCaT and B16F10 Cells

“…As generally known [ 70 ], the radical scavenging activity of these plants is strongly related to their content in phenolic compounds such as catechin, cinnamic acid, ferulic acid, p-coumaric acid and sinapic acid. As Table 1 shows, Pp-EE was found to exhibit 273 mg of gallic acid equivalent/g extract, superior to the value found for extracts of P. atrosanguinea , P. rugulosa , and P. fruticosa that revealed to have 21.75, 77.58, and 148.4 mg, respectively [ 65 , 66 , 71 ]. Considering these points, we suggest that Potentilla spp., in particular P. paradoxa , can be used as a strong anti-aging remedy with high phenolic contents linked to strong antioxidative capacity.…”