High-Precision Detection of Cellular Drug Response Based on SERS Spectrum and Multivariate Statistical Analysis

Abstract

The rapid development of personalized medicine places high demands on the control of drug dose and cellular drug response to provide patients with better curative effects and low side effects. To solve the problem of low detection accuracies of the cell-counting kit-8 (CCK8) method, a detection method based on surface-enhanced Raman spectroscopy (SERS) of cell-secreted proteins was adopted to evaluate the concentration of the anticancer drug cisplatin and the cellular drug response of nasopharyngeal carcinoma. CNE1 and NP69 cell lines were used to evaluate cisplatin response. The results showed that the combination of the SERS spectrum with principal component analysis–linear discriminant analysis could detect the difference in the response of cisplatin with a concentration difference of 1 μg/mL, which considerably exceeded that of CCK8. In addition, the SERS spectral peak intensity of the cell-secreted proteins strongly correlated with the cisplatin concentration. Furthermore, the mass spectrum of the secreted proteins of the nasopharyngeal carcinoma cells was analyzed to verify the results obtained using the SERS spectrum. The results demonstrated that SERS of secreted proteins has great potential for high-precision detection of chemotherapeutic drug response.

Effective strategies to enhance the diagnosis and treatment of RCC: The application of biocompatible materials

“…This approach offers a rapid, sensitive, and multi-analysis capable biosensor based on the SERS technique for the detection of tumor exosomes, presenting a potential tool for clinical cancer screening [ 47 ]. In their study, Lucaciu et al illustrated that the utilization of serum label-free surface-enhanced Raman spectroscopy (SERS) coupled with multivariate analysis holds potential for achieving precise discrimination of renal cell carcinoma (RCC), thereby offering promise for the non-invasive and early detection of this disease [ 48 ].…”

Deciphering biomolecular complexities: the indispensable role of surface-enhanced Raman spectroscopy in modern bioanalytical research

“…Illustrating this, Wu and colleagues showcased the real-time monitoring of binding dynamics of an emergent anti-cancer agent with its target protein, furnishing critical data that is poised to shape future drug optimization endeavors. 25 Cumulatively, these research endeavors not only substantiate the immense utility of SERS within proteomic analytics but also chart an inspirational trajectory for future investigative pursuits within this domain.…”