High‐power, red‐light‐emitting diode irradiation enhances proliferation, osteogenic differentiation, and mineralization of human periodontal ligament stem cells via ERK signaling pathway

Yamauchi, Nobuhiro; Taguchi, Yoichiro; Kato, Hirohito; Umeda, Makoto

doi:10.1002/jper.17-0365

Cited by 38 publications

(28 citation statements)

References 48 publications

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“…RUNX2 is an early transcription factor regulating the osteogenic differentiation progress (Kato et al, 2016; Li, Chen, & Zhang, 2015). OSX is also an early osteoblast transcription factor and has a vital role in the differentiation of preosteoblasts into immature osteoblasts (Yamauchi, Kato, & Umeda, 2018). Our results indicated that the expression of OPN, RUNX2, OSX, and COL‐1 were upregulated in CTP‐CM‐induced hPDLSCs as compared with those in the control group.…”

Section: Discussionmentioning

confidence: 99%

CTP‐CM enhances osteogenic differentiation of hPDLSCs via NF‐κB pathway

Wang

et al. 2020

Oral Diseases

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Objective The conditioned medium of calcined tooth powder (CTP‐CM) is a type of biomimetic mineralized material and well contributing to bone healing and bone formation in vivo. However, little is known about the effect of CTP‐CM on human periodontal ligament stem cells (hPDLSCs) as well as the underlying mechanisms. Methods ALP activity assay was conducted to select the concentration with the highest ALP level, which was used for the following experiments. Cell proliferation was measured by cell counting kit‐8 assay and flow cytometry analysis. Expression levels of osteogenic markers in CTP‐CM‐induced hPDLSCs were evaluated with real‐time quantitative reverse transcription polymerase chain reaction (qRT‐PCR), immunofluorescence staining, and Western blot. Mineralization of CTP‐CM‐induced hPDLSCs was evaluated by alizarin red staining. Furthermore, the involvement of NF‐κB pathway was examined by immunofluorescence staining and Western blot. Results 20 μg/ml was selected for the further experiments. Functional studies demonstrated that CTP‐CM exerted almost no influence on the proliferation of hPDLSCs and CTP‐CM increased the osteogenic differentiation of hPDLSCs. Mechanistically, CTP‐CM leads to activation of NF‐κB signaling pathway. When treated with BMS345541, the osteogenic differentiation of CTP‐CM‐treated hPDLSCs was significantly attenuated. Conclusion CTP‐CM can promote the osteogenic differentiation of hPDLSCs via activating NF‐κB pathway.

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Section: Discussionmentioning

confidence: 99%

CTP‐CM enhances osteogenic differentiation of hPDLSCs via NF‐κB pathway

Wang

et al. 2020

Oral Diseases

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“…LLLT induced positive effects on cell proliferation were also observed in cultured normal human keratinocytes, fibroblasts, osteoblasts, mesenchymal and cardiac stem cells, endothelial and HeLa cells. ROS production following LLLT has been demonstrated to be able to induce preodontoblasts as well as preosteoblasts proliferation [4,13,15,21,22,26,47,48,[67][68][69][70], in which Runx2 and Osterix mRNA expression result enhanced by ERK1/2 pathway [71].…”

Section: Lllt Effects On Cellular Respiration and Redox Statementioning

confidence: 99%

Near infrared low‐level laser therapy and cell proliferation: The emerging role of redox sensitive signal transduction pathways

Migliario

Sabbatini

Mortellaro

et al. 2018

Journal of Biophotonics

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Lasers devices are widely used in various medical fields (eg, surgery, dermatology, dentistry, rehabilitative medicine, etc.) for different applications, ranging from surgical ablation of tissues to biostimulation and pain relief. Laser is an electromagnetic radiation, which effects on biological tissues strongly depends on a number of physical parameters. Laser wavelength, energy output, irradiation time and modality, temperature and tissue penetration properties have to be set up according to the clinical target tissue and the desired effect. A less than optimal operational settings, in fact, could result in a null or even lethal effect. According to the first law of photobiology, light absorption requires the presence of a specific photoacceptor that after excitation could induce the activation of downstream signaling pathways. Low-level lasers operating in the red/near infrared portion of the light spectra are generally used for biostimulation purposes, a particular therapeutic application based on the radiant energy ability to induce nonthermal responses in living cells. Biostimulation process generally promotes cell survival and proliferation. Emerging evidences support a low-level laser stimulation mediated increase in "good" reactive oxygen species, able to activate redox sensitive signal transduction pathways such as Nrf-2, NF-kB, ERK which act as key redox checkpoints.

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“…These cells possess biological characteristics, which resemble the mesenchymal stem cells (MSCs) and are of great value to the study of periodontal tissue regeneration . Therefore, for periodontal tissue regeneration, PDLSCs are considered as the most promising seed cells and have own unique advantages in oral and maxillofacial tissue regeneration . To optimize seed cells, gene regulation must be accomplished, which requires understanding the mechanism of PDLSC differentiation and regulation.…”

Section: Introductionmentioning

confidence: 99%

TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3

Tian

et al. 2019

J of Cellular Biochemistry

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Objective This study aims to offer insights about the biological influence of TAZ, which is a transcriptional coactivator containing a PDZ‐binding motif, upon the apoptosis, proliferation, and osteogenic differentiation of human periodontal ligament stem cells (h‐PDLSCs). Methods We used the green fluorescence protein lentivirus infection system to knockdown or overexpress TAZ in h‐PDLSCs. 5‐ethynyl‐2’‐deoxyuridine (EdU) staining detected the proliferative activity, and h‐PDLSC apoptosis was analyzed by Annexin V‐APC staining. TAZ knockdown or overexpression was performed to determine the osteogenic differentiation function of TAZ during the osteogenic induction of h‐PDLSCs. The molecular mechanism of TAZ in the promotion of h‐PDLSC osteogenesis was also explored. The chemical inhibitor of SMAD2/3 SIS3 HCL was used to identify the effects in vitro osteogenic differentiation and bone formation in h‐PDLSCs overexpressing TAZ. Results TAZ overexpression resulted in enhanced cell rapid multiplication, which increased the expression of messenger RNA in stemness‐related genes. By comparison, TAZ knockdown reduced proliferative activity and increased the apoptosis of h‐PDLSCs. After the 7‐day osteogenic induction period, alkaline phosphatase activity in the TAZ‐overexpression group was significantly increased, and mineralized nodules increased significantly after osteogenic induction for 21 days. Similarly, osteoblast differentiation of h‐PDLSCs was impaired after TAZ knockdown. However, the osteogenic potential of the group exposed to the p‐SMAD3 inhibitor was restored to its original level. Conclusion Hippo/TAZ plays a positive role inside the proliferation, stemness maintenance, and osteogenic specialization of h‐PDLSCs, and the specific downstream factor of osteogenic differentiation is SMAD3.

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High‐power, red‐light‐emitting diode irradiation enhances proliferation, osteogenic differentiation, and mineralization of human periodontal ligament stem cells via ERK signaling pathway

Abstract: The results of this study show that 650-nm high-power, red, LED irradiation increases PDLSCs proliferation, and osteogenic differentiation and mineralization, mediated by ERK1/2 activation. These findings suggest that LED may be a useful tool for periodontal tissue regeneration.

Cited by 38 publications

References 48 publications

CTP‐CM enhances osteogenic differentiation of hPDLSCs via NF‐κB pathway

CTP‐CM enhances osteogenic differentiation of hPDLSCs via NF‐κB pathway

Near infrared low‐level laser therapy and cell proliferation: The emerging role of redox sensitive signal transduction pathways

TAZ promotes the proliferation and osteogenic differentiation of human periodontal ligament stem cells via the p‐SMAD3

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