High-Power 365 nm UV LED Mercury Arc Lamp Replacement for Photochemistry and Chemical Photolithography

Hölz, Kathrin; Lietard, Jory; Somoza, Mark M.

doi:10.1021/acssuschemeng.6b02175

Cited by 71 publications

(47 citation statements)

References 43 publications

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“…A high-power 365 nm UV-LED (Nichia NVSU333A) serves as a light source (40). The layout of the array is determined by the pattern of UV irradiation, which triggers the selective removal of the photolabile 2-(2-nitrophenyl)-propyloxycarbonyl (NPPOC) 5΄-OH protecting group.…”

Section: Methodsmentioning

confidence: 99%

Mapping the affinity landscape of Thrombin-binding aptamers on 2′F-ANA/DNA chimeric G-Quadruplex microarrays

et al. 2017

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In situ fabricated nucleic acids microarrays are versatile and very high-throughput platforms for aptamer optimization and discovery, but the chemical space that can be probed against a given target has largely been confined to DNA, while RNA and non-natural nucleic acid microarrays are still an essentially uncharted territory. 2΄-Fluoroarabinonucleic acid (2΄F-ANA) is a prime candidate for such use in microarrays. Indeed, 2΄F-ANA chemistry is readily amenable to photolithographic microarray synthesis and its potential in high affinity aptamers has been recently discovered. We thus synthesized the first microarrays containing 2΄F-ANA and 2΄F-ANA/DNA chimeric sequences to fully map the binding affinity landscape of the TBA1 thrombin-binding G-quadruplex aptamer containing all 32 768 possible DNA-to-2΄F-ANA mutations. The resulting microarray was screened against thrombin to identify a series of promising 2΄F-ANA-modified aptamer candidates with Kds significantly lower than that of the unmodified control and which were found to adopt highly stable, antiparallel-folded G-quadruplex structures. The solution structure of the TBA1 aptamer modified with 2΄F-ANA at position T3 shows that fluorine substitution preorganizes the dinucleotide loop into the proper conformation for interaction with thrombin. Overall, our work strengthens the potential of 2΄F-ANA in aptamer research and further expands non-genomic applications of nucleic acids microarrays.

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Section: Methodsmentioning

confidence: 99%

Mapping the affinity landscape of Thrombin-binding aptamers on 2′F-ANA/DNA chimeric G-Quadruplex microarrays

et al. 2017

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“…Second, the cost of UV-LEDs was far below that of the high-pressure mercury arc lamp, including initial investment, power consumption, and maintenance (Supplementary Information Table S2). 43 Third, the color change of the capillary microreactor took place when it was exposed to the irradiation of the high-pressure mercury arc lamp for long time (Figure 5b irradiation of the high-pressure mercury arc lamp, many unidentified byproducts were formed according to GC analysis, differing from the UV-LEDs counterpart. However, as shown in Figure 5c, the reaction rate of NBD irradiated by the high-pressure mercury arc lamp was much greater than that irradiated by UV-LEDs, which may be due to the higher photon flux emitted by the high-pressure mercury arc lamp.…”

Section: Photosensitizers and Solventsmentioning

confidence: 99%

A UV‐LEDs based photomicroreactor for mechanistic insights and kinetic studies in the norbornadiene photoisomerization

et al. 2019

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In this work, we report a simple and inexpensive UV-LEDs based photomicroreactor assembly constructed by commercially available components. The photoisomerization of norbornadiene to quadricyclane was selected to validate this novel photomicroreactor design. Mass transport limitation was eliminated and indicated by dimensionless numbers Fo and Da II , and photons loss was evaluated considering the absorption and reflection of the microreactor walls. The solvents, photosensitizers, and light sources selections were optimized for achieving better photochemical performance and mechanistic insights. The detailed comparison between the high-pressure mercury arc lamp and the UV-LEDs strip revealed great potential of UV-LEDs as appealing light source for photochemical transformations. Moreover, the reaction mechanism was thoroughly discussed and illustrated by the Jablonski diagram indicating electronic states transitions. According to possible intermediate steps, a kinetic model was proposed with the reaction rate constant being correlated with the photon flux, which is valuable for process optimization and further understanding reaction mechanisms.

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“…With the recent advancement of UV-LEDs and the availability of various wavelengths, researchers are moving towards an easier and more convenient UV-LED light source exposure system [10,11]. It has also been demonstrated how a specific wavelength of UV-LED can be a good replacement for traditional UV lamps that contains multiple-wavelength peaks [12] and can achieve success in maskless photolithography as well [13]. However, these UV-LED light sources were controlled manually and exposure is allowed only in the vertical direction.…”

Section: Introductionmentioning

confidence: 99%

3D Microlithography Using an Integrated System of 5-mm UV-LEDs with a Tilt-Rotational Sample Holder

et al. 2020

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This paper demonstrates a 3D microlithography system where an array of 5 mm Ultra Violet-Light Emitting Diode (UV-LED) acts as a light source. The unit of the light source is a UV-LED, which comes with a length of about 8.9 mm and a diameter of 5 mm. The whole light source comprises 20 × 20 matrix of such 5 mm UV-LEDs giving a total number of 400 LEDs which makes it a very favorable source with a large area for having a batch production of the desired microstructures. This light source is able to give a level of precision in microfabrication which cannot be obtained using commercial 3D printers. The whole light source performs continuous rotational movement once it is turned on. This can also move up and down in a vertical direction. This multidirectional light source also comprises a multidirectional sample holder. The light source teaming up with the multidirectional sample holder highly facilitates the process of fabrication of a huge range of 3D structures. This article also describes the different levels of characterization of the system and demonstrates several fabricated 3D microstructures including high aspect ratio vertical micro towers, twisted turbine structures, triangles, inclined pillar ‘V’ structures, and hollow horn structures as well.

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High-Power 365 nm UV LED Mercury Arc Lamp Replacement for Photochemistry and Chemical Photolithography

Cited by 71 publications

References 43 publications

Mapping the affinity landscape of Thrombin-binding aptamers on 2′F-ANA/DNA chimeric G-Quadruplex microarrays

Mapping the affinity landscape of Thrombin-binding aptamers on 2′F-ANA/DNA chimeric G-Quadruplex microarrays

A UV‐LEDs based photomicroreactor for mechanistic insights and kinetic studies in the norbornadiene photoisomerization

3D Microlithography Using an Integrated System of 5-mm UV-LEDs with a Tilt-Rotational Sample Holder

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