High potential of adhesion to biotic and abiotic surfaces by opportunistic Staphylococcus aureus strains isolated from orthodontic appliances

Merghni, Abderrahmen; Nejma, Mouna Ben; Dallel, Ines; Tobji, Samir; Amor, A. Ben; Janel, Sébastien; Lafont, Frank; Aouni, Mahjoub; Mastouri, Maha

doi:10.1016/j.micpath.2015.11.009

Cited by 21 publications

(18 citation statements)

References 36 publications

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“…The microbiological finding of Staphylococcus spp. on PTFE surfaces in our study agrees with what has been shown in a series of investigations by Merghni and coworkers (Merghni et al, 2016; Merghni et al, 2017; Merghni, Ben Nejma, Hentati, Mahjoub, & Mastouri, 2014). The authors evaluated the adhesive ability of oral staphylococcal strains isolated from the oral cavity and orthodontic appliances or in vitro from different abiotic surfaces commonly encountered in dental practices.…”

Section: Discussionsupporting

confidence: 93%

Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers

Turri

Čirgić

Shah

et al. 2020

Clinical & Exp Dental Res

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Objectives This clinical randomized study aimed to evaluate the early plaque formation on nonresorbable polytetrafluoroethylene (PTFE) membranes having either a dense (d‐PTFE) or an expanded (e‐PTFE) microstructure and exposed to the oral cavity. Material and Methods Twelve individuals were enrolled in this study. In a split‐mouth design, five test membranes (e‐PTFE) with a dual‐layer configuration and five control membranes (d‐PTFE) were bonded on the buccal surfaces of posterior teeth of each subject. All study subjects refrained from toothbrushing during the study period. Specimens were detached from the teeth at 4 and 24 hr and subjected to viability counting, confocal microscopy, and scanning electron microscopy. Plaque samples were harvested from neighboring teeth at baseline, 4, and 24 hr, as control. Wilcoxon signed rank test was applied. Results No bond failure of the membranes was reported. Between the early and late time points, viable bacterial counts increased on all membranes, with no difference between the test and control. The number of Staphylococcus spp. decreased on the tooth surfaces and increased on both membranes overtime, with a significant difference compared to teeth. The total biomass and average biofilm thickness of live and dead cells were significantly greater at the d‐PTFE barriers after 4 hr. Conclusion This study demonstrated that the e‐PTFE membrane was associated with a lesser degree of biofilm accumulation during the initial exposure compared to the d‐PTFE membrane. The present experimental setup provides a valuable toolbox to study the in vivo behavior of different membranes used in guided bone regeneration (GBR).

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Section: Discussionsupporting

confidence: 93%

Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers

Turri

Čirgić

Shah

et al. 2020

Clinical & Exp Dental Res

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“…It frequently colonizes human skin and mucosa and conditionally causes a wide range of infections, leading to various clinical symptoms, including boils, impetigo, arthritis and sepsis. Staphylococcus aureus , an important opportunistic pathogen, causes a variety of infectious diseases [28, 29]. It has become prevalent because of the use of drugs, such as antibiotics, and the development of MRSA.…”

Section: Discussionmentioning

confidence: 99%

The effect of immunoregulation of Streptococcus lactis L16 strain upon Staphylococcus aureus infection

Wang

Gong

et al. 2017

BMC Microbiol

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Background Staphylococcus aureus is an important pathogen that causes various infections in medical facilities. However, resistance to multiple drugs has made this infection difficult to manage. Thus, new therapeutic strategies are urgently needed to solve this worldwide public health problem. The Streptococcus lactis L16 strain was isolated from the fermented hot chili sauce. To explore whether it can be used as a protective agent against S. aureus infection, we designed a mouse model of S. aureus infection to evaluate the therapeutic potency of S. lactis. Mice were grouped into pre-(P) and post-(T) S. aureus infection groups following oral administration of S. lactis L16. The protection and treatment effects were assessed by examining body weight, internal organ weight, serum cytokines and intestinal secretory IgA alternations.ResultOral administration of the S. lactis L16 strain reduced the loss of body weight in mice post-infection and alleviated infection-induced hepatomegaly. In particular, the PL16 group (protection with L16) showed more effective resistance to S. aureus than the TL16 group (treatment with L16). The level of serum cytokine interferon gamma following oral administration of the L16 strain was remarkably increased during infection, as were interleukin-4 levels during convalescence. The probiotic L16 strain induced more sIgA production than S. aureus. ConclusionOur data suggest that S. lactis L16 is an effective strain with anti-Staphylococcus activity. By regulating the Th1/Th2 response, S. lactis can effectively reduce lesions from infection, indicating its therapeutic potential in overcoming antibiotic resistance in this mouse infection model that mimics infections observed in humans.

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“…S. pneumoniae and S. aureus are common commensals of the upper respiratory tract in children and adolescents [14,100,108]. This fact is relevant since orthodontic patients are mainly children and adolescents and the high genotypic expression of peculiar genes (icaA/icaD) is important for S. aureus in the colonization of orthodontic appliances [109]. Recently, RNA-Seq data permit the analysis of active transcripts, assigned to antibiotics and toxic compounds, of the supragingival dental plaque biofilm in healthy subjects [110].…”

Section: Staphylococcus Aureus and Mrsamentioning

confidence: 99%

Infection Control in Dentistry and Drug-Resistant Infectious Agents: A Burning Issue. Part 1

Barenghi¹,

Barenghi²,

Blasio³

2020

Surgical Infections - Some Facts

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Using molecular biological methods and retrospective investigations, some outbreaks in dental settings have been proven to be caused by mainly blood-borne viruses and water-borne bacteria. Nowadays, drug-resistant bacteria seem further hazards taking into account the worldwide overuse of antibiotics in dentistry, the limited awareness on infection prevention guidelines, and the lapses and errors during infection prevention (reported in more detail in Part 2). We chose MRSA and VRE as markers since they are considered prioritized bacteria according antibiotic resistance threats. Antibiotic-resistant bacterial infections inside of dental setting are relevant, and we argue about some hazards in dentistry, including dedicated surgeries. MRSA has a key role for its colonization in patients and dental workers, presence on gloves, resistance (days-months on dry inanimate surfaces), the contamination of different clinical contact surfaces in dental settings, the ability of some strains to produce biofilm, and finally its estimated low infective dose. For better dental patient and healthcare personnel safety, we need evidence-based guidelines to improve education and training initiatives in surgery.

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High potential of adhesion to biotic and abiotic surfaces by opportunistic Staphylococcus aureus strains isolated from orthodontic appliances

Cited by 21 publications

References 36 publications

Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers

Early plaque formation on PTFE membranes with expanded or dense surface structures applied in the oral cavity of human volunteers

The effect of immunoregulation of Streptococcus lactis L16 strain upon Staphylococcus aureus infection

Infection Control in Dentistry and Drug-Resistant Infectious Agents: A Burning Issue. Part 1

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