High Pigment Mutants of Tomato Exhibit High Sensitivity for Phytochrome Action

Peters, Janny L.; Tuinen, A. van; Adamse, P.; Kendrick, Richard E.; Koornneef, M.

doi:10.1016/s0176-1617(89)80024-0

Cited by 65 publications

(48 citation statements)

References 11 publications

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“…Alba et al (2000) showed that phytochrome-mediated light signal transduction was required for normal ripe fruit pigmentation but did not affect other ripening attributes. Tomato high-pigment (hp1 and hp2) mutants, characterized by increased green fruit and leaf chlorophyll in addition to increased total ripe fruit carotenoids, have been shown to be hypersensitive to light (Peters et al, 1989). Ectopic expression of an oat phytochrome in tomato resulted in phenotypes similar to those exhibited by hp1 and hp2, further emphasizing the role of light in fruit carotenoid accumulation (Boylan and Quail, 1989).…”

Section: The Role Of Light In Fruit Ripeningmentioning

confidence: 99%

Genetic Regulation of Fruit Development and Ripening

Giovannoni

2004

THE PLANT CELL ONLINE

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850

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Section: The Role Of Light In Fruit Ripeningmentioning

confidence: 99%

Genetic Regulation of Fruit Development and Ripening

Giovannoni

2004

THE PLANT CELL ONLINE

1,189

850

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“…Anthocyanin was extracted from 20 seedlings, as described by Peters et al (1989), and the amount was estimated from the A 535 value. Total chlorophyll was estimated as described previously (Kurata and Yamamoto, 1997).…”

Section: Chemical Assaysmentioning

confidence: 99%

petit1, a Conditional Growth Mutant of Arabidopsis Defective in Sucrose-Dependent Elongation Growth

Kurata

Yamamoto

1998

Plant Physiology

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The hypocotyl of Arabidopsis is well suited for the analysis of cell elongation because it elongates without cell division. We have isolated a new class of recessive mutants, petit1 (pet1), which are defective in aspects of hypocotyl elongation. The short-hypocotyl phenotype of pet1 is caused by shortened cells. The cells of the elongation zone of the hypocotyl are often deformed. pet1 also shows defects in elongation of the roots, flower stalk, leaves, petals, pedicels, and siliques, and these defects cannot be repaired by the application of auxin, gibberellin, brassinolide, or an inhibitor of ethylene biosynthesis. The short-hypocotyl phenotype of pet1 is pronounced only in growth medium supplemented with sucrose, which has promotive effects on hypocotyl elongation. In pet1 this effect is much reduced, causing the sucrose-dependent shorthypocotyl phenotype of pet1. pet1 accumulates more soluble sugars than the wild type and also shows more intensive iodo-starch staining in the cotyledon and hypocotyl. These results indicate that PETIT1 is involved in a sugar-dependent elongation process that may include correct assembly of expanding cell wall architecture.

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“…For the anthocyanin assay, samples of siliques, dry seeds, 10 day-old seedlings, rosette leaves (1-month-old plants) or floral buds were taken and extracted with acidified (1% HCl, w/v) methanol for 48 h in the dark with shaking. After Folch partitioning, the absorbance of the top phase was determined with a Beckman DU 640 spectrophotometer at 535 nm, essentially as described by Peters et al (1989). For thin-layer chromatography, approximately 0.1 g of the immature seeds (10 days after flowering (DAF) for the mutant and 17 DAF for the Columbia ecotype), were crushed and suspended in 50 µl of methanol containing 1% HCl, mixed with the same volume of 4 M HCl, and then placed in boiling water for 40 min as described by Strack and Wray (1989).…”

Section: Mutant Isolationmentioning

confidence: 99%

A new Arabidopsis mutant induced by ion beams affects flavonoid synthesis with spotted pigmentation in testa.

et al. 1997

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A new stable mutant of Arabidopsis thaliana with a spotted pigment in the seed coat, named anthocyanin spotted testa (ast), was induced by carbon ion irradiation. The spotted pigmentation of ast mutant was observed in immature seeds from 1-2 days after flowering (DAF), at the integument of the ovule, and spread as the seed coat formed. Anthocyanin accumulation was about 6 times higher in ast mutant than in the wild-type at 6 DAF of the immature seeds, but was almost the same in mature dry seeds. A higher anthocyanin accumulation was not observed in the seedlings, leaves or floral buds of ast mutant compared with the wild-type, which suggests that a high accumulation of anthocyanins is specific to the seed coat of the immature ast seeds. Reciprocal crosses between ast mutant and the wild-type indicated that ast is a single recessive gene mutation and segregates as a delayed inheritance. The results of crossing with tt7 and ttg mutants also confirmed that the AST gene is probably a regulatory locus that controls flavonoid biosynthesis. A mapping analysis revealed that the gene is located on chromosome I and is closely linked to the SSLP DNA marker nga280 with a distance of 3.2 cM. AST has been registered as a new mutant of Arabidopsis.

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High Pigment Mutants of Tomato Exhibit High Sensitivity for Phytochrome Action

Cited by 65 publications

References 11 publications

Genetic Regulation of Fruit Development and Ripening

Genetic Regulation of Fruit Development and Ripening

petit1, a Conditional Growth Mutant of Arabidopsis Defective in Sucrose-Dependent Elongation Growth

A new Arabidopsis mutant induced by ion beams affects flavonoid synthesis with spotted pigmentation in testa.

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