High pH during Trisodium Phosphate Treatment Causes Membrane Damage and Destruction of<i>Salmonella enterica</i>Serovar Enteritidis

Balamurugan, S.; Khachatourians, George G.; Korber, Darren R.

doi:10.1128/aem.69.1.122-129.2003

Cited by 71 publications

(58 citation statements)

References 37 publications

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“…alkaline treatment, heating, chemical treatment and ultrasound) can damage microbial cell membranes (Goodford, 1971;Virto et al, 2005;Tachibana et al, 1999;Sampathkumar et al, 2003). For example, Sampathkumar et al (2003) found that high pH (>10.0) during trisodium phosphate treatment causes membrane damage of S. enterica. The damage of cell membranes in the EPS extraction should thus be detected.…”

Section: Damage Of Cell Membranes In Eps Extractionmentioning

confidence: 99%

“…Previous studies had reported the damage of microbial cell structures. Sampathkumar et al (2003) found that high pH levels during trisodium phosphate treatment caused membrane damage in Salmonella enterica serovar enteritidis and resulted in the loss of cell viability; Shen et al (2009) assessed freeze-thaw and high-pressure low-temperature-induced damage to Bacillus subtilis cells with flow cytometry and Pulgarin et al (2012) studied the damage of cell walls in Escherichia coli by TiO 2 suspensions. In some studies of EPS extraction from activated sludge, the damage to microbial cell structures was evaluated by the detection of cellular materials or measuring the composition of cellular structures.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge

Guo

Liu

Xiao

2014

Journal of Biotechnology

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a b s t r a c t Extracellular polymeric substances (EPS) are susceptible to contamination by intracellular substances released during the extraction of EPS owing to the damage caused to microbial cell structures. The damage to cell walls and cell membranes in nine EPS extraction processes of activated sludge was evaluated in this study. The extraction of EPS (including proteins, carbohydrates and DNA) was the highest using the NaOH extraction method and the lowest using formaldehyde extraction. All nine EPS extraction methods in this study resulted in cell wall and membrane damage. The damage to cell walls, evaluated by 2-keto-3-deoxyoctonate (KDO) and N-acetylglucosamine content changes in extracted EPS, was the most significant in the NaOH extraction process. Formaldehyde extraction showed a similar extent of damage to cell walls to those detected in the control method (centrifugation), while those in the formaldehydeNaOH and cation exchange resin extractions were slightly higher than those detected in the control. N-acetylglucosamine was more suitable than KDO for the evaluation of cell wall damage in the EPS extraction of activated sludge. The damage to cell membranes was characterized by two fluorochromes (propidium iodide and FITC Annexin V) with flow cytometry (FCM) measurement. The highest proportion of membrane-damaged cells was detected in NaOH extraction (26.54% of total cells) while membranedamaged cells comprised 8.19% of total cells in the control.

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Section: Damage Of Cell Membranes In Eps Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge

Guo

Liu

Xiao

2014

Journal of Biotechnology

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“…Further, our results indicated that nonanal could damage the plasma membrane of spores (Figure 2). Sampathkumar et al [43] observed that high pH may cause membrane damage and destruction of Salmonella enterica Serovar Enteritidis. Pinto et al [44] found that PI penetrated over 95% of Candida albicans cells following a short incubation period with 2.5 μl/ml clove essential oil.…”

Section: Discussionmentioning

confidence: 99%

Use of Nonanal-wax as Postharvest Fungicide of Tomato against <i>Botrytis cinerea</i>

Zhang¹,

Zeng²,

Sun³

et al. 2017

JFNR

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The antifungal activity of nonanal against Botrytis cinerea, one of the most important postharvest diseases of tomato gray mold, was tested by in vitro and in vivo experiments. Results of in vivo tests demonstrated that wax + nonanal treatment significantly decreased the incidence of gray mold during the entire storage period. After 8 d of storage, the disease incidences in Wax + nonanal (1×, 4× or 10× MFC)-treated fruits were 46.7%, 56.7%, 73.3%, respectively, in contrast to 100% of the control fruits. Loss of membrane integrity was examined and quantified under 10×MFC nonanal condition by the method of propidium iodide fluorescent staining. Wax + nonanal (10×MFC) treatment remarkably increased antioxidant enzyme activities, such as catalase (CAT), superoxidase dismutase (SOD), peroxidase (POD) and phenylalanine ammonia lyase (PAL). Meanwhile, this treatment (10×MFC) evidently exhibited a delayed decline in antioxidant enzyme activities. Furthermore, nonanal treatment retained the fruit quality of tomatoes because it reduced the coloration index and weight loss and retained fruit firmness. No significant differences were found between the pH, Firmness and total soluble solid (TSS) content for all treatment under the same storage time. Our results suggest that nonanal can be considered as a good alternative to conventional fungicides in controlling the decay of tomato fruits.

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“…Because bacterial flagella mediate attachment to both host cells and inanimate surfaces, carvacrol may therefore interfere with attachment of bacteria to host or environmental surfaces [38]. Polyphosphates are also thought to promote detachment of pre-biofilm salivary proteins to tooth enamel or bacteria to food surfaces [39,40]. Combinations of polyP and EOs may therefore have promise as inhibitors of bacterial attachment through a combination of overt physiological effects on cells and physical effects on the surfaces to which they are attached.…”

Section: Discussionmentioning

confidence: 99%

Sodium polyphosphate and polyethylenimine enhance the antimicrobial activities of plant essential oils

Wright¹,

Brehm-Stecher²

2016

ScienceOpen Research

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Plant extracts have been used for millennia for treatment of disease, with much recent interest focusing on the antimicrobial activities of plant essential oils (EOs). Although EOs are active against common microbial pathogens, their effective use as topical, environmental, or food antimicrobials will require EO-based formulations with enhanced antimicrobial activities. In this study, two polyionic compounds, sodium polyphosphate (polyP, a polyanion) and polyethylenimine (PEI, a polycation), were evaluated for their abilities to enhance the antimicrobial activities of six EOs against the human pathogens Escherichia coli O157:H7, Salmonella enterica subsp. enterica ser. Minnesota, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus, and Candida albicans. EOs tested were cinnamon, clove, regular and redistilled oregano, and two types of thyme oil. EOs were examined via disk diffusion and broth microdilution, either alone or in the presence of subinhibitory levels of polyP or PEI. Both polyP and PEI were found to be effective enhancers of EO activity against all strains examined, and calculation of fractional inhibitory indices for select EO/organism pairings demonstrated that true synergy was possible with this enhancement approach. Experiments with a deep-rough strain of S. Minnesota probed the role of the outer membrane in both intrinsic resistance to EOs and enhancement by polyions. The use of polyP and PEI for boosting the antimicrobial activities of EOs may eventually facilitate the development of more effective EO-based antimicrobial treatments for use in applications such as wound treatment, surface disinfection, or as generally recognized as safe antimicrobials for use in foods or on food contact surfaces.

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High pH during Trisodium Phosphate Treatment Causes Membrane Damage and Destruction ofSalmonella entericaSerovar Enteritidis

Cited by 71 publications

References 37 publications

Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge

Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge

Use of Nonanal-wax as Postharvest Fungicide of Tomato against <i>Botrytis cinerea</i>

Sodium polyphosphate and polyethylenimine enhance the antimicrobial activities of plant essential oils

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