High performance thin-layer chromatography (HPTLC) analysis of cannabinoids in cannabis extracts

Liu, Yifan; Brettell, Thomas A.; Victoria, Justin; Wood, Matthew R.; Staretz, Marianne E.

doi:10.1016/j.forc.2020.100249

Cited by 16 publications

(27 citation statements)

References 36 publications

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“…As silylation agents are harmful for GC injection port and column, additional evaporation to dryness is frequently performed, followed by dissolution in solvent, e.g., n- hexane [ 84 ] or MeCN [ 95 ]. If no derivatization is performed, EtAc [ 120 , 126 , 127 ] and EtOH [ 66 , 123 ] are the most commonly used solvents for reconstitution prior to GC analysis.…”

Section: Analytical Methods For Phytocannabinoid Profilingmentioning

confidence: 99%

Analytical Techniques for Phytocannabinoid Profiling of Cannabis and Cannabis-Based Products—A Comprehensive Review

et al. 2022

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Cannabis is gaining increasing attention due to the high pharmacological potential and updated legislation authorizing multiple uses. The development of time- and cost-efficient analytical methods is of crucial importance for phytocannabinoid profiling. This review aims to capture the versatility of analytical methods for phytocannabinoid profiling of cannabis and cannabis-based products in the past four decades (1980–2021). The thorough overview of more than 220 scientific papers reporting different analytical techniques for phytocannabinoid profiling points out their respective advantages and drawbacks in terms of their complexity, duration, selectivity, sensitivity and robustness for their specific application, along with the most widely used sample preparation strategies. In particular, chromatographic and spectroscopic methods, are presented and discussed. Acquired knowledge of phytocannabinoid profile became extremely relevant and further enhanced chemotaxonomic classification, cultivation set-ups examination, association of medical and adverse health effects with potency and/or interplay of certain phytocannabinoids and other active constituents, quality control (QC), and stability studies, as well as development and harmonization of global quality standards. Further improvement in phytocannabinoid profiling should be focused on untargeted analysis using orthogonal analytical methods, which, joined with cheminformatics approaches for compound identification and MSLs, would lead to the identification of a multitude of new phytocannabinoids.

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Section: Analytical Methods For Phytocannabinoid Profilingmentioning

confidence: 99%

Analytical Techniques for Phytocannabinoid Profiling of Cannabis and Cannabis-Based Products—A Comprehensive Review

et al. 2022

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“…A total of 89 samples of suspected cannabis products (77 vegetation, 4 edible gummies, 4 e-cigarettes) were obtained from two crime laboratories and prepared as described in a previous publication [2] . These samples were prepared by personnel at the crime laboratories for analysis by GC–MS.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The extracts were analyzed as received and applied to the HPTLC plates. Case sample application format can be found in (S10) in a previous publication [2] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…A total of 89 samples of suspected cannabis products (77 vegetation, 4 edible gummies, 4 e-cigarettes) were obtained from two crime laboratories and prepared as described previously [2] . The first three case sample plates from both xylene:hexane:diethylamine (25:10:1) (XHDa) and 6% diethylamine:toluene (6DaT) mobile phase systems, as well as Pennsylvania State Police case samples were shown in the previous article [2] . Here we report the rest of the case sample plate images on both mobile phase systems ( Fig.…”

Section: Data Descriptionmentioning

confidence: 99%

“…The data described in this article is related to a previous published research article “High Performance Thin-Layer Chromatography (HPTLC) analysis of cannabinoids in cannabis extracts” by Liu, et al. (2020) [2] .…”

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High Performance Thin-Layer Chromatography (HPTLC) data of Cannabinoids in ten mobile phase systems

et al. 2020

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This article contains data from triplicate analyses of ten different TLC mobile phase systems from the analysis of 11 cannabinoid standards using HPTLC. The data includes 1) example plate images for 8 mobile phases during evaluation; 2) example table of calculated average, standard deviation, CV% of R F for hexane:acetone (87:13) system during evaluation; 3) images of all case sample plates. The validation of the two chosen mobile phases were done according to the SWGDRUG validation requirements [1] . This data can be used for choosing an alternative mobile phase TLC system for the analysis of cannabinoids. The data described in this article is related to a previous published research article “High Performance Thin-Layer Chromatography (HPTLC) analysis of cannabinoids in cannabis extracts” by Liu, et al. (2020) [2] .

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Extraction solvent selection for Cannabis sativa L. by efficient exploration of cannabinoid selectivity and phytochemical diversity

Tzimas,

Petrakis,

Halabalaki

et al. 2023

Phytochemical Analysis

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IntroductionCannabis sativa L. is attracting worldwide attention due to various health‐promoting effects. Extraction solvent type is critical for the recovery of bioactive compounds from the plant, especially cannabinoids. However, the choice of solvent is varied and not adequately warranted elsewhere, causing confusion in involved fields.ObjectiveThe present work aimed to investigate the effect of extraction solvent on C. sativa (hemp) with regard to cannabinoid recovery and phytochemical profile of the extracts, considering most of the related solvents.MethodologyThe majority of solvents reported for C. sativa (n = 14) were compared using a representative hemp pool. Quantitative results for major and minor cannabinoids were rapidly and reliably obtained using ultrahigh‐performance liquid chromatography coupled with photodiode array detection (UPLC‐PDA). In parallel, high‐performance thin‐layer chromatographic (HPTLC) fingerprinting was employed, involving less toxic mobile phase than in relevant reports. Various derivatisation schemes were applied for more comprehensive comparison of extracts.ResultsDifferential selectivity towards cannabinoids was observed among solvents. MeOH was found particularly efficient for most cannabinoids, in addition to solvent systems such as n‐Hex/EtOH 70:30 and ACN/EtOH 80:20, while EtOH was generally inferior. For tetrahydrocannabinol (THC)‐type compounds, EtOAc and n‐Hex/EtOAc 60:40 outperformed n‐Hex, despite its use in the official EU method. Solvents that tend to extract more lipids or more polar compounds were revealed based on HPTLC results.ConclusionCombining the observations from UPLC quantitation and HPTLC fingerprinting, this work allowed comprehensive evaluation of extraction solvents, in view of robust quality assessment and maximised utilisation of C. sativa.

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High performance thin-layer chromatography (HPTLC) analysis of cannabinoids in cannabis extracts

Cited by 16 publications

References 36 publications

Analytical Techniques for Phytocannabinoid Profiling of Cannabis and Cannabis-Based Products—A Comprehensive Review

Analytical Techniques for Phytocannabinoid Profiling of Cannabis and Cannabis-Based Products—A Comprehensive Review

High Performance Thin-Layer Chromatography (HPTLC) data of Cannabinoids in ten mobile phase systems

Extraction solvent selection for Cannabis sativa L. by efficient exploration of cannabinoid selectivity and phytochemical diversity

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