High-Performance Thin Layer Chromatography (Hptlc) Fingerprinting Pattern of Mangrove Avicennia Marina

Objective: The present study investigates the antimicrobial activities of eight different mangrove plants and also the development of their intra-relationship through cluster analysis. Methods: The dried and powdered leaves of different mangrove species were extracted by cold maceration process with water, methanol, and chloroform. The antimicrobial activity was done using the agar well diffusion method. The cluster analysis of the mangrove plants was analyzed by MINITAB Release 13.1. Results: The order of extraction yield for each mangrove species was methanol > chloroform > water. The methanol extract of Suaeda maritima, Avicennia marina, Avicennia officinalis, and chloroform extract of Sonneratia apetala gave the highest inhibition zones of 19 mm, 19 mm, 19.33 mm, and 19.33 mm, respectively, against Bacillus subtilis. The methanol extract of Ceriops decandra, Xylocarpus granatum, and Bruguiera gymnorrhiza found the highest inhibition zones of 21.67 mm, 22 mm, and 20.3 mm, respectively, against Escherichia coli, Shigella flexneri, and Staphylococcus aureus, respectively. The endangered and endemic species Heritiera fomes gave the highest result (18 mm) against both for Micrococcus luteus and B. subtilis in methanolic extract. The maximum zone of inhibition of fungal strains was found against Botrytis cinerea (15 mm), Fusarium oxysporum (16.33 mm), and Rhizopus oryzae (13.33 mm) with the methanol extract of X. granatum, S. apetala, and C. decandra, respectively. Cluster analysis of 8 mangrove species based on different bacterial pathogens was also shown where it demonstrated their intra-relationship against some bacterial pathogens. Conclusion: From this study, it may be concluded that mangrove plants can be used to discover bioactive natural products.

Section: Phytochemical Screeningsupporting

confidence: 76%

Study of Antimicrobial Potentiality of Eight Different Mangrove Species and Development of Their Intra-Relationship Through Cluster Analysis

Kader

Ghosh

Sinha

2020

“…HPTLC is used to assess, compare, and quantify the reservoirs of secondary metabolites in different plants and also can be used in fingerprinting as conducted by Dawane and Fulekar [1] in which the results obtained by HPTLC fingerprinting method were found reliable, accurate, and economical for identification and authentication of Avicennia marina mangrove species from other similar mangroves, in a single TLC plate run.…”

Section: Introductionmentioning

confidence: 99%

HPTLC METHOD FOR ESTIMATION AND QUANTIFICATION OF Β-Sitosterol FROM IN-VIVO AND IN-VITRO SAMPLES OF MERREMIA AEGYPTIA AND MERREMIA DISSECTA

Joshi

Meena

Mishra

et al. 2020

Objective: A normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed and validated for estimation and quantitation of beta-sitosterol from the methanolic fraction of different plant parts of two medicinally important plants viz. Merremia aegyptia and Merremia dissecta. These plants have been reported to possess antimicrobial, antioxidant, and anti-inflammatory activities. Methods: Chromatographic separation of beta-sitosterol from the methanolic extracts of plant parts of M. aegyptia and M. dissecta was performed on TLC aluminum plates pre-coated with silica gel 60F254 using a suitable mobile phase. The densitometric scanning was done after derivatization at ????-580 nm for ????-sitosterol. Result: Only M. dissecta leaf sample was reported to contain ????-sitosterol (4.6 ng/μl), whereas other samples such as seed, stem, and callus extracts of M. aegyptia and M. dissecta did not showed its presence. Conclusion: The developed HPTLC method is simple, rapid, and precise and can be used for routine analysis and quantification of ????-sitosterol and other useful plant bioactives that are phytopharmaceutically important.

“…Several pharmacopeia containing monographs of the plant materials describe only the physicochemical characters. Hence, the modern methods describing the identification and quantification of active constituents in the plant material may be useful for proper standardization of herbs and its formulations [7,8].…”

Section: Introductionmentioning

confidence: 99%

High-Performance Thin-Layer Chromatography Fingerprint Profile of Bauhinia Tomentosa Linn. Leaves

Balabhaskar

Krishnamurthy

2018

Objective: Chromatographic fingerprint is an effective method for doing the fingerprinting of a plant species. In this study, high-performance thinlayer chromatography (HPTLC) analysis of Bauhinia tomentosa was done in n-hexane, chloroform, and ethanol extracts. Methods:The extract of leaves was developed using toluene:ethyl acetate:formic acid:glacial acetic acid (7:3:0.1:0.1) for n-hexane, toluene:ethyl acetate:formic acid (6:2:0.5) for chloroform, and chloroform:methanol:formic acid (8:1.5:0.2) for ethanol extract as mobile phase using standard procedures and scanned under ultraviolet at 254 nm, 366 nm, and 520 nm. Results:The HPTLC fingerprinting results showed several peaks with different R f values. The HPTLC fingerprinting of n-hexane extract at 266 nm showed 15 peaks. The HPTLC fingerprinting of chloroform extract at 520 nm showed 22 peaks. The HPTLC fingerprinting of the ethanol extract at 366 nm showed 13 peaks. Conclusion:These fingerprinting results will be helpful in the identification and authentication of the species and also to identify new bioactive components in this medicinal plant.