High performance thin layer chromatography-densitometry method based on picosecond laser-induced fluorescence for the analysis of thioridazine and its photoproducts

Tozar, Tatiana; Boni, Mihai; Andrei, Ionuț Relu; Pascu, Mihail Lucian; Staicu, Angela

doi:10.1016/j.chroma.2021.462488

Cited by 4 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Por esto, una alternativa para la identificación de sustancia orgánicas es la Cromatografía En Capa Fina (CCF) y se caracteriza por ser un procedimiento sencillo que puede llevarse a cabo con éxito sin instrumentación sofisticada [5]. A esto se suma la aplicación de métodos de densitometría para el análisis de las manchas en las placas cromatográficas [6]. Sin embargo, esto también puede traer consigo costos adicionales, frente a esto, hoy en día se han desarrollado diversas alternativas para determinar diversos compuestos orgánicos por CCF mediante análisis o procesamiento de imágenes [7], [8].…”

Section: Introductionunclassified

Development of a rapid method for the quantification of methamidophos and chlorpyrifos by Thin Layer Chromatography in soils using image processing

Gonzales-Condori¹,

Reynoso-Espinoza²,

Yucra-Condori³

et al. 2022

Proceedings of the 20th LACCEI International Multi-Conference for Engineering, Education and Technology: “Education, Research A

View full text Add to dashboard Cite

The objective of this research was to develop a rapid method to identify and quantify chlorpyrifos and methamidophos in soils by Thin Layer Chromatography. A mobile phase of chloroform:acetone (80:20) was used for methamidophos and for chlorpyrifos a mixture of chloroform: cyclohexane (60:40) was used as mobile phase. The developer consisted of a 0.12 M ethanolic solution of silver nitrate. The stains were processed using "Image J" software. The results show retention factors "Rf" of 0.19 and 0.82 for methamidophos and chlorpyrifos respectively. Both methods for the determination of chlorpyrifos and methamidophos are linear with coefficients of determination R 2 greater than 0.99, precise with RSD less than 15 % and accurate with %R close to 100 %. The analysis of agricultural soil samples of Garlic and Onion showed the presence of chlorpyrifos with concentrations of 0.067 and 0.047 mg/kg respectively. Also, methamidophos was found in alfalfa agricultural soils with a concentration of 0.011 mg/kg. With the results found, it is demonstrated that image processing with "Image J" for the analysis of stains on silica gel plates developed with silver nitrate in the analysis of organophosphates can be taken into account for demonstrative studies for educational purposes and routine tests in toxicology and environment.

show abstract

Section: Introductionunclassified

Development of a rapid method for the quantification of methamidophos and chlorpyrifos by Thin Layer Chromatography in soils using image processing

Gonzales-Condori¹,

Reynoso-Espinoza²,

Yucra-Condori³

et al. 2022

Proceedings of the 20th LACCEI International Multi-Conference for Engineering, Education and Technology: “Education, Research A

View full text Add to dashboard Cite

show abstract

“…The development of low-cost and disposable paper-based microfluidic chips, combined with novel assay formats, can reduce the testing time and the complexity of diagnostic testing [15,16]. A fluorescence detection thin-layer chromatography separation method was also used to detect microorganisms [17]. The detection accuracy can be improved by using photomultiplier tubes and wavelength filters to collect fluorescence signals, and using integrators to process the output fluorescence intensity [18,19].…”

Section: Introductionmentioning

confidence: 99%

Design of UVA Ultraviolet Disinfection System for Nutrient Solution Residual Liquid and Development of Microbial Online Monitoring System

2022

View full text Add to dashboard Cite

If the nutrient solution used in the hydroponic system is recycled and reused without disinfection, the plant diseases are likely to spread. The current disinfection system still cannot conduct online monitoring of microorganisms at the same time as disinfection. In this paper, a UVA ultraviolet disinfection system and an online microbial monitoring system are proposed, which can conduct online monitoring at the same time as disinfection. This system includes the design of the disinfection system, the microbial online detection system and the microfluidic chip. The practical performance of the disinfection system and the microfluidic chip was verified by means of simulation and experiment. The relationship between the working power (P) of the UVA ultraviolet sterilizer used and its irradiance (Ee) is P = 29.98 Ee. The direct influencing factor of the ultraviolet disinfection rate of the nutrient solution residual liquid was the ultraviolet light irradiation dose. When the power of the ultraviolet lamp (radiation flux) is 30 W, the optimum wavelength is close to 300 nm, and the absorbance value is approximately 0.07. The error between bioluminescence detection and laboratory culture detection error is 0.002. The disinfection method and microfluidic chip proposed in this paper can be used in a greenhouse hydroponic system to reduce the impact of harmful microorganisms in the nutrient solution return on plants, and improve the effect of the return solution.

show abstract

Reversible polarity-switch of thin-layer chromatography by photo-induction with multi-regulation in spatial dimension

Shang,

Zhang,

et al. 2024

Talanta

View full text Add to dashboard Cite

High performance thin layer chromatography-densitometry method based on picosecond laser-induced fluorescence for the analysis of thioridazine and its photoproducts

Cited by 4 publications

References 27 publications

Development of a rapid method for the quantification of methamidophos and chlorpyrifos by Thin Layer Chromatography in soils using image processing

Development of a rapid method for the quantification of methamidophos and chlorpyrifos by Thin Layer Chromatography in soils using image processing

Design of UVA Ultraviolet Disinfection System for Nutrient Solution Residual Liquid and Development of Microbial Online Monitoring System

Reversible polarity-switch of thin-layer chromatography by photo-induction with multi-regulation in spatial dimension

Contact Info

Product

Resources

About