High-performance thin layer chromatography-based phytochemical and bioactivity characterisation of anticancer endophytic fungal extracts derived from marine plants

Assessment of Biological Activities of Fungal Endophytes Derived Bioactive Compounds Isolated from Amoora rohituka

“…Other factors include the synergistic action of other class of compounds that showed potential antioxidant activity [ 38 , 39 , 40 , 41 ]. Some previous studies have also shown inconsistent correlation between phenolic content and antioxidant activity [ 42 , 43 ]. However, A. flavus have been reported with significant antioxidant activity and with a positive correlation with the phenolic content.…”

High‐performance thin‐layer chromatography based approach for bioassay and ATR–FTIR spectroscopy for the evaluation of antioxidant compounds from Asparagus racemosus Willd. aerial parts

“…The intensity of bands was increased by heating the derivatized plates at 100-105 C for 10-15 min followed by evaluation of plates under both white light (Figure 2f) and at UV 366 nm (Figure 2g). Greyish and pale blue color bands were visualized under white light and at UV 366 nm (Figure 2f, g), respectively, after anisaldehyde/sulfuric acid derivatization, which indicated the presence of polyphenolic groups (Lim et al, 2021). Purple-violet and reddish or blue colors were observed under white light and at UV 366 nm (Figure 2f & 2g), respectively, which is common for triterpenes.…”

“…Anisaldehyde/sulfuric acid is the most commonly used reagent for the detection of a wide range of phytochemicals, which gives specific colors after derivatization. The appearance of violet, blue, red, grey or green bands was observed for phenols, terpenes, sugars and steroids, respectively, with anisaldehyde/sulfuric acid derivatization where bands are visible in both white light and at 366 nm (Lim et al, 2021). The intensity of bands was increased by heating the derivatized plates at 100-105 C for 10-15 min followed by evaluation of plates under both white light (Figure 2f) and at UV 366 nm (Figure 2g).…”

Endophytic fungi: Benefits for plants and biotechnological potential

“…Similar bioactive compounds produced from endophytes, were related in many research papers: quinones (Hu et al, 2001); lignans (Puri et al, 2006); alkaloids (Yadav et al, 2014); lactones (Elfita et al, 2014;Liu et al, 2018); isocumarins (Wu et al, 2019), steroids (Parthasarathy et al, 2020); and phenols (Yadav et al, 2014;Khiralla et al, 2015, Bhabhardwaj et al, 2015Parthasarathy et al, 2020). These bioactive compounds showed several pharmacological activities, such as, antidiabetic (Zhang et al, 1999), antiviral (Guo et al, 2000), anti-inflammatory (Weber et al, 2004), antitumour (Li et al, 2005), immunosuppressive (Xu et al, 2020), and anticancer (Lim et al, 2021). These benefits indicate a wide applicability of bioactive compounds from endophytic fungi.…”