High-performance thin-layer chromatographic analysis of antioxidants present in different parts of Saraca asoca (Roxb.) de Wilde

Saha, Jayita; Mukherjee, Sourav; Gupta, Bhaskar

doi:10.1016/j.jopr.2013.10.004

Cited by 21 publications

(24 citation statements)

References 10 publications

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“…Numerous literature reports establish the antibacterial properties of methanolic, ethnolic, acetone and aqueous extracts of bark, dried flower buds and leaves of S. asoca [26][27][28][29][30][31][32][33][34][35][36][37][38][39]61 . 31,61,65 .…”

Section: Antibacterialmentioning

confidence: 99%

“…Lignin glycosides such as lyoniside, nudiposide, 5-methoxy-9--xylopyranosyl, isolariciresinol and schizandriside; flavonoids such as catechin, epicatechin, epiafzelechin-(48)-epicatechin, procyanidin B2, deoxyprocyanidin B, leukocyanidins and leucopelargonidin 61,81,82 ; and leucopelargonidin glucoside 83 have been reported from S. asoca bark 14,21,48,50,61,81,82 . Antioxidants such as polyphenolics, gallic acid and ellagic acid have also been described from S. asoca bark 14,21,38,43,[47][48][49]83 .…”

Section: Barkmentioning

confidence: 99%

Section: Leavesmentioning

confidence: 99%

“…They have been reported to contain alkaloids, steroids, flavonoids, tannins, saponins, terpenoids, polyphenolics, glycosides and many carbohydrates 38,43,46,[84][85][86] . The antioxidant activity of the leaf extracts has been described to be due to the presence of polyphenolics such as gallic acid and ellagic acid 38 . Flavonoids such as quercetin, -sitoterol, ceryl alcohol, and glucosides such as quercetin-3-O--rhamnoside and kaemferol-3-O--Lrhamnoside have been reported from S. soaca leaves 14,21,33,38,49,72,81,83,84 .…”

Section: Leavesmentioning

confidence: 99%

“…They are especially known for the presence of many fatty acids such as oleic, palmitic, stearic, linolenic and linoleic acids; glucosides such as quercetin-3-O-P-D-glucoside, apigenin-7-O-p-Dglucoside, pelargonidin-3,5-diglucoside and cyanidine-3,5-diglucoside; steroids such as p-and y-sitosterols; flavonoids such as quercetin, leucocyanidin, and polyphenolics such as gallic acid and ellagic acid 14,21,38,84 .…”

Section: Flowersmentioning

confidence: 99%

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Phytomedicinal Importance of <i>Saraca asoca</i> (Ashoka): An Exciting Past, an Emerging Present and a Promising Future

Singh¹,

Krishna²,

Kamalraj³

et al. 2015

Current Science

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Since the dawn of civilization, natural resources have remained the mainstay of various remedial approaches of humans vis-à-vis a large number of illnesses. Saraca asoca (Roxb.) de Wilde (Saraca indica L.) belonging to the family Caesalpiniaceae has been regarded as a universal panacea in old Indian Ayurvedic texts and has especially been used to manage gynaecological complications and infections besides treating haemmorhagic dysentery, uterine pain, bacterial infections, skin problems, tumours, worm infestations, cardiac and circulatory problems. Almost all parts of the plant are considered pharmacologically valuable. Extensive folkloric practices and ethnobotanical applications of this plant have even lead to the availability of several commercial S. asoca formulations recommended for different indications though adulteration of these remains a pressing concern. Though a wealth of knowledge on this plant is available in both the classical and modern literature, extensive research on its phytomedicinal worth using state-of-the-art tools and methodologies is lacking. Recent reports on bioprospecting of S. asoca endophytic fungi for industrial bioproducts and useful pharmacologically relevant metabolites provide a silver lining to uncover single molecular bio-effectors from its endophytes. Here, we describe socio-ethnobotanical usage, present the current pharmacological status and discuss potential bottlenecks in harnessing the proclaimed phytomedicinal worth of this prescribed Ayurvedic medicinal plant. Finally, we also look into the possible future of the drug discovery and pharmaceutical R&D efforts directed at exploring its pharma legacy.

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Section: Antibacterialmentioning

confidence: 99%

Section: Barkmentioning

confidence: 99%

Section: Leavesmentioning

confidence: 99%

Section: Leavesmentioning

confidence: 99%

Section: Flowersmentioning

confidence: 99%

See 3 more Smart Citations

Phytomedicinal Importance of <i>Saraca asoca</i> (Ashoka): An Exciting Past, an Emerging Present and a Promising Future

Singh¹,

Krishna²,

Kamalraj³

et al. 2015

Current Science

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Combination of DNA isolation and RP-HPLC analysis method for bark samples of Saraca asoca and its adulterant

2017

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DNA fingerprinting singly or in combination with phytochemical analysis is ideal for quality control of crude plant-based drugs. However, when the source material is tannin rich stem bark, extraction of DNA by conventional methods becomes challenging. In such cases, phytochemical profiling serves as very useful tool for its identification. The work herein described a method for simultaneous DNA isolation and phytochemical extraction for downstream analysis and applications from dried bark powder of Saraca asoca and commercial samples of this crude drug as well as from those of Polyalthia longifolia, its most common adulterant. It is a modified CTAB-based method which involves a pre-extraction step by soaking samples overnight in de-ionized water followed by filtration. The residues in the filter paper were used for DNA isolation and dried filtrate was used for Reverse Phase-High-Performance Liquid Chromatography analysis. Results revealed that genomic DNA isolated was PCR amplifiable with Inter Simple Sequence Repeat and Start Codon Targeted markers. Phenolic compounds of catechin, epicatechin, and gallic acid were detected from the above dried filtrate. The method is simple, reliable and it requires small amount of sample with an option of integrating both phytochemical and DNA-based profiling, from the same starting material. Therefore, the present method could be useful for further potential applications such as quality control assessment of S. asoca products.

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Monitoring of catechin content by high performance liquid chromatography during the development of seed and pods of Saraca asoca (Roxb.) De Willd

Dahiya

Kumar

Bolleddu

et al. 2023

Vegetos

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High-performance thin-layer chromatographic analysis of antioxidants present in different parts of Saraca asoca (Roxb.) de Wilde

Cited by 21 publications

References 10 publications

Phytomedicinal Importance of <i>Saraca asoca</i> (Ashoka): An Exciting Past, an Emerging Present and a Promising Future

Phytomedicinal Importance of <i>Saraca asoca</i> (Ashoka): An Exciting Past, an Emerging Present and a Promising Future

Combination of DNA isolation and RP-HPLC analysis method for bark samples of Saraca asoca and its adulterant

Monitoring of catechin content by high performance liquid chromatography during the development of seed and pods of Saraca asoca (Roxb.) De Willd

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