High performance size exclusion chromatography of starch with dimethyl sulfoxide as the mobile phase

Abstract

synopsisTwo types of high performance size exclusion chromatographic (SEC) columns were tested for the characterization of starch samples: pbndagel and Aquapore. The mobile phase was dimethyl sulfoxide (DMSO) containing 0.03 M sodium nitrate maintained at 80°C. The results indicated that both pBondagel and Aquapore can be used to determine the relative molecular weight of starch. The exclusion limits for these columns exceed ten million. Preliminary results using pstyragel are also reported. Examples of SEC analysis of starch, including starch with anionic groups, are given.

Starch Molecular Mass and Size by Size‐Exclusion Chromatography in DMSO‐LiBr Coupled with Multiple Angle Laser Light Scattering

“…The curve is linear between 40,000 and 8,000,000 when an ionic component (LiBr) is included. This result is consistent with previous studies of SEC of carbohydrate polymers on silica-based column packing material (Chuang and Snyder 1987). A review of semirigid polymer gel packing materials suggested that ionic modifiers are usually necessary to minimize the effects of packing material surface chemistry and ionic components of the polymer (Meehan 1995).…”

“…Dimethylsulfoxide (DMSO) or aqueous DMSO solutions have been used to solubilize cereal starches in preparation for analysis of total starch (Libby 1970) (AACC 1995), nuclear magnetic resonance (Gidley 1985, Kasemsuwan andJane 1996) and SEC (Kobayashi et al 1985, Chuang andSnyder 1987). DMSO has been used previously as the mobile phase in SEC of starch utilizing silica-based column packing material (Stone and Krasonski 1981).…”

Size Exclusion Chromatography of Dextrans in Dmso as Eluent

“…First of all, the DMSO offers numerous advantages: it is a strong hydrogen bond acceptor and, secondly, the interaction parameter between DMSO/dextran is 20 times as high as that of H 2 O/dextran. An additional retention is observed in the silica columns with blends of DMSO/H 2 O as eluent, 24,18,20,23 we can also notice additional peak. 16 DMSO can also be used at high temperature to promote the solubilization of crystalline polymers; indeed, some polysaccharides such as starch and starch derivatives, which do not well dissolve in H 2 O, can be more easily dissolved in DMSO.…”

“…14,15 However, in many cases, polysaccharides are not totally water-soluble, this is why we also tested pure DMSO and blends of DMSO/H 2 O. Different column packages have been used: silicas grafted diol, 17,18,19,20,21 dextran bisacrylamide, 22 styrene divinylbenzene 23 normally used for organic SEC. The dextran dissolution enthalpy is also higher in DMSO than in H 2 O.…”

“…16 DMSO can also be used at high temperature to promote the solubilization of crystalline polymers; indeed, some polysaccharides such as starch and starch derivatives, which do not well dissolve in H 2 O, can be more easily dissolved in DMSO. 17,18,20 So, the purpose of this study was to find the best elution conditions for analysing polysaccharides such as starch. 14 The SEC columns available for DMSO-based SEC have to be relatively strong to withstand the high pressures due to the high viscosity of DMSO.…”