High performance reversed‐phase chromatography of the triglycerides from human plasma lipoproteins

Perkins, E. G.; Hendren, David J.; Pelick, Nicholas; Bauer, John E.

doi:10.1007/bf02535227

Cited by 14 publications

(6 citation statements)

References 9 publications

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“…In the same publication five TAG pairs were also not separated (LnLO/ALO, LnOO/AOO, AOP/ALS, LOP/LLS and OOP/LOS). Insufficient resolution of these pairs was also reported in other studies of mammalian tissues 81, 91, 96–99, 101. This problem can be overcome by MS, as fragmentation of acylglycerols depends on the composition of their FA residues.…”

Section: Lc‐msmentioning

confidence: 66%

“…After LC, Plattner et al collected the eluent volume corresponding to peaks of each ECN and separated the FA of each ECN group after transesterification by GC. This peak collection was also done by other groups 81–83 to ensure the correct identification of the peaks in the LC chromatogram. Furthermore, Plattner et al used a C18 column which is still commonly used.…”

Section: Lcmentioning

confidence: 99%

“…TAG mixtures of natural fats are less complex than those of biological tissues like muscle and liver cells. Perkins et al 81 adapted TAG measurement to human lipoprotein classes. They adequately separated critical pairs containing linolenic (Ln), L, O and P isocratically with a mixture of acetone/acetonitrile (63.6:36.4) and two C18 columns in series within 45 min.…”

Section: Lcmentioning

confidence: 99%

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Determination of acylglycerols from biological samples with chromatography‐based methods

Hellmuth¹,

Uhl²,

Segura³

et al. 2011

J of Separation Science

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Lipids are the most diverse class of metabolites in mammalian physiology and dysregulation of lipid metabolism is linked to various diseases. Alterations in acylglycerols, a major class of lipids in plasma and adipose tissue, are involved in the pathogenesis of obesity and type 2 diabetes. Therefore, determination of acylglycerols is important to depict and unravel cellular mechanisms related to pathological outcomes, and specific molecular species of acylglycerols might be promising biomarker candidates. The variety of acylglycerols can be characterized in different ways. Enzymatic assays enable the determination of total tri- or diacylglycerols showing a possible relation to diseases, but they do not allow clarification of molecular mechanism. While gas chromatography can provide an overview of the fatty acid composition of total or separated lipids, a very detailed description of the individual molecular acylglycerol species is possible via liquid chromatography, particularly when combined with mass spectrometry. This review describes the determination of acylglycerols considering recent developments, with a focus on mammalian serum/plasma and tissue.

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Section: Lc‐msmentioning

confidence: 66%

Section: Lcmentioning

confidence: 99%

Section: Lcmentioning

confidence: 99%

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Determination of acylglycerols from biological samples with chromatography‐based methods

Hellmuth¹,

Uhl²,

Segura³

et al. 2011

J of Separation Science

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“…A number of techniques have been used for TAG analysis including gas chromatography (Skorepa et al 1979), both normal (Hamilton and Comai 1984) and reversed-phase high performance liquid chromatography (RP-HPLC) (Perkins et al 1982;Podlaha and Toregard 1982) and supercritical fluid chromatography (Tso et al 1999). As foodstuffs and oils contain a complex mixture of TAGs, the use of reversed-phase high performance liquid chromatography (RP-HPLC) coupled to mass spectrometry (LC/MS) or tandem mass spectrometry (LC-MS/MS) has proved to be the most widely used analytical technique, usually in conjunction with either atmospheric pressure chemical ionization (APCI) or electrospray ionization (ESI).…”

Section: Introductionmentioning

confidence: 99%

Analysis of changes in triacylglycerol ratios in mouse liver and plasma in response to a liver X receptor agonist

Scullion¹,

Edwards²,

McKinnon³

et al. 2011

Metabolomics

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An LC-MS method was developed for the analysis of triacylglycerols (TAG) in mouse liver extracts and plasma samples. C57 Mice were treated with two LXR agonists that have been shown to upregulate TAGs, T0901317 (T1317) or Org 264693 and compared to vehicle dosed animals. The dose used was 30 mg kg(-1), once daily, with three different dose regimes; 24 H, 48 H and 5 day. The TAG ratios measured were C52: 2/C54: 3 and C52: 3/C54: 4, which corresponded to a decrease in the palmitate and an increase in oleate composition of the TAGs. A significant change in the C52: 2/C54: 3 ratio was observed with all dose regimes and a good correlation was obtained between liver and plasma samples. In a separate study, the same compounds were dosed to LXR alpha and LXR beta knock-out (KO) mice at 30 mg kg(-1), once daily, for 5 days. The LXR beta KO mice showed similar TAG ratio changes to the C57 mice, whereas the LXR alpha KO mice showed no change in TAG ratios versus vehicle dosed animals. Measurements of lipid liability in response to an LXR agonist are typically made by measuring total liver TAG levels, which here, only showed a significant effect after the 48 H and 5 day dose regimes. By using a ratio measurement analysis could be performed on plasma samples, greatly simplifying the sample preparation procedure, without the requirement for either calibration curves or an internal standard

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“…Separation of triglycerides by revers~phase high-performance liquid chromatography (HPLC) with various highefficiency packed columns and mebile phases was well documented in the late 1970s and early 1980s (1)(2)(3)(4)(5)(6)(7)(8)(9). These columns allowed separation of triglyoefides not only by their acyl chainlength but also by the degree of unsaturation within the same chainlengtb_ Rules for the chromatographic behavior of triglyceride molecular species have been developed based on equivalent carbon number (ECN) (9), theoretical carbon number (TCN) (10) and matrix model (11).…”

mentioning

confidence: 99%

Behavior of diglycerides and conjugated fatty acid triglycerides in reverse‐phase chromatography

Chang¹,

Conkerton²,

Chapital³

et al. 1994

J Americ Oil Chem Soc

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The behavior of conjugated fatty acid triglycerides and diglycerides on revers~phase chromatography was studied. Trieleostearin is a geometric isomer of trilinoleni~ The conjugated double bond arrangement in trieleostearin enhances its hydrophobic interaction with the statienary phase and causes it to be eluted later than trilinolenin. In separation of "critical pairs" ef tri-and diglycerides, diglycerides elute later than triglycerides due to the longer fatty acid constituent. Position isemers of 1,2-and 1,3diglycerides can be separated by reverse-phase high-performance liquid chromatography.

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High performance reversed‐phase chromatography of the triglycerides from human plasma lipoproteins

Cited by 14 publications

References 9 publications

Determination of acylglycerols from biological samples with chromatography‐based methods

Determination of acylglycerols from biological samples with chromatography‐based methods

Analysis of changes in triacylglycerol ratios in mouse liver and plasma in response to a liver X receptor agonist

Behavior of diglycerides and conjugated fatty acid triglycerides in reverse‐phase chromatography

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