High performance reversed phase chromatography of cholesterol and cholesteryl esters of human plasma lipoproteins

Perkins, E. G.; Hendren, David J.; Bauer, John E.; El-Hamdy, Ali H.

doi:10.1007/bf02534908

Cited by 27 publications

(4 citation statements)

References 16 publications

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“…The steryl ester fraction is a complex mixture consisting of a variety of sterols esterified to various fatty acids, and analysis of such mixtures is hindered by the presence of high proportions of triacylglycerols and other components of oils (Gordon and Griffith, 1992). Methods used so far for the analysis of steryl esters generally involve their isolation by preparative thin-layer chromatography or column chromatography followed by gas chromatography (GC) (Gordon and Griffith, 1992;Kuksis et al, 1986), high-performance liquid chromatography (HPLC) (Gordon and Griffith, 1992;Perkins et al, 1981), or coupled HPLC/GC (Grob et al, 1990). Recently, preparative HPLC has been used for the isolation of steryl esters, which is followed by their analysis using GC (Miller and Gordon, 1995).…”

Section: Introductionmentioning

confidence: 99%

Alteration of Sterols and Steryl Esters in Vegetable Oils during Industrial Refining

Ferrari

Esteves

Mukherjee

et al. 1997

J. Agric. Food Chem.

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Steryl esters of fatty acids have been isolated from vegetable oils by rapid chromatography on a short column of silica gel and quantitatively determined by HPLC on RP-18 columns using an evaporative light scattering detector that was calibrated with β-sitosteryl oleate as external standard. The steryl esters isolated according to the above technique from corn, soybean, and rapeseed oils at different stages of industrial refining have been analyzed for their constituent sterols and fatty acids by GC. Moreover, the total sterols were analyzed by GC. In all three oils a substantial part of the sterols occurred as steryl esters. Some alterations in the composition of the constituent sterols and fatty acids of the steryl esters were observed during industrial processing. Keywords: Industrial fat refining; HPLC; sterols; steryl esters; vegetable oils

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Section: Introductionmentioning

confidence: 99%

Alteration of Sterols and Steryl Esters in Vegetable Oils during Industrial Refining

Ferrari

Esteves

Mukherjee

et al. 1997

J. Agric. Food Chem.

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“…Over the years, gas chromatography (GC) has been used to characterize cholesterol and its derivatives. , Usually, the GC analysis of a complex mixture of cholesterol derivatives provides poor resolution due to their structural similarity. Therefore, capillary columns with high efficiency are necessary.…”

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confidence: 99%

Capillary Electrochromatography of Cholesterol and Its Ester Derivatives

et al. 2000

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Separation of cholesterol and its ester derivatives using micellar electrokinetic chromatography is a challenge due to the extreme hydrophobicity of these compounds. In this work, an isocratic capillary electrochromatography (CEC) method has been developed to separate a complex mixture of cholesterol and its 12-ester derivatives. The proportions of mobile phase (tetrahydrofuran, acetonitrile, water), as well as the effects of acid modifiers, buffer concentrations, voltage, and temperature on the separation of cholesterol derivatives were investigated. Addition of a polymeric surfactant, poly(sodium N-undecanoyl-L-glycinate), to the mobile phase reduced migration time and improved resolution of the analytes. The CEC method developed allows baseline separation of a complex mixture of cholesterol and 12 ester derivatives in less than 40 min. Finally, the method is applied to the characterization of cholesterol, cholesterol linoleate, and cholesterol oleate extracted from atherosclerotic plaque deposits in the arterial walls of a human aorta.

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“…Reversed-phase high-performance liquid chromatography (RP-HPLC) with an octadecylsilanyl packing material has been widely used for the structural analysis of triacylglycerols (1), retinoyl fatty acyl esters (2)(3)(4), and steryl fatty acyl esters (5,6). Miwa et al (7,8) have also separated the molecular species ofjojoba wax esters with RP-HPLC in combination with gas chromatography/mass spectrometry (GC/MS).…”

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Characterization of wax esters in the roe oil of amber fish,Seriola aureovittata

Joh

Brechany

Christie³

1995

J Americ Oil Chem Soc

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The wax esters of the roe oil of the amber fish, Seriola aureovittata, have been resolved by high-performance liquid chromatography (HPLC) in the silver-ion mode. Each of the fractions collected was transmethylated, and the fatty acids and alcohols were identified by gas chromatography/mass spectrometry (GC/MS) as the picolinyl esters and nicotinates, respectively. Their compositions were determined by GC. The fatty acid composition is complex, and the main components are C18:1n-9 (35.5 mo[%), C22:6n-3 (20.3 mo[%), and C16:1n-7 (10.7 mol%), while fatty alcohols are limited to saturated (C16:0, 60.3 mol%; C18:0, 15.3 mol%; C14:0, 5.1 mol%) and monoenoic alcohols (C18:1n-9, 6.5 mol%; C16:1n-7, 4.5 tool%) with traces (<0.1 mol%) of polyunsaturated fatty alcohols such as C20:3n-3, C20:4n-6, C20:5n-3, and C22:5n-3. Silver-ion HPLC exhibited excellent resolution in which fractions were resolved on the basis of the number and configuration of double bonds as well as the distribution pattern between the acid and alcohol moieties of the molecules with a given number of double bonds. The main wax ester fraction are those of monoenoic acid-saturated alcohol species, hexaenoic acid/saturated alcohol species, and pentaenoic acid/saturated alcohol species. Appreciable specificity was observed in the esterification of fatty acids with alcohols, and surprisingly, no saturated acid-monoenoic alcohol species were detected. JAOCS 72, 707-713 (1995).

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High performance reversed phase chromatography of cholesterol and cholesteryl esters of human plasma lipoproteins

Cited by 27 publications

References 16 publications

Alteration of Sterols and Steryl Esters in Vegetable Oils during Industrial Refining

Alteration of Sterols and Steryl Esters in Vegetable Oils during Industrial Refining

Capillary Electrochromatography of Cholesterol and Its Ester Derivatives

Characterization of wax esters in the roe oil of amber fish,Seriola aureovittata

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