High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula

Stodůlková, Eva; Man, Petr; Kolařík, Miroslav; Flieger, Miroslav

doi:10.1016/j.chroma.2010.08.009

Cited by 21 publications

(17 citation statements)

References 28 publications

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“…Secondary metabolites are known to have a large impact on measured genome size (PI binding to DNA; e.g., 37). Geosmithia produce a spectrum of secondary metabolites , so fixation accompanied by washing of secondary metabolites could play an important role in the quality of fluorescent measurements. The MA+ fixative was then evaluated for four incubation times: overnight, 60, 30, and 15 min (Table ).…”

Section: Resultsmentioning

confidence: 99%

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Application of flow cytometry for genome size determination in Geosmithia fungi: A comparison of methods

et al. 2014

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Genome size has played an important role in the evolution of plants and animals because changes in genome size seem to accompany if not facilitate evolutionary adaptation to environmental conditions. Flow cytometry (FCM) is a widespread method for determining genome size thanks to its high accuracy and speed of measurements. Nevertheless, only a few comparative studies of FCM methods exist in the field of mycology, and reviews are absent. In this study, we compared the suitability of several concentrations and RNAse A incubation times, fixatives and buffers for estimating genome size in fungi. We chose the genus Geosmithia as a model filamentous fungus. We also introduced a new standard, Aspergillus fumigatus CEA10, to determine absolute genome size. We found FCM to be an appropriate method for measuring genome size in fungi, but optimization steps showed that incorrect propidium iodide staining of nuclei can overestimate genome size due to cytoplasmic staining. We identified fixation with methanol:glacial acetic acid (3:1 v/v), 10% DMSO, 0.1% Triton-X 100, and 5 mM EDTA in combination with Tris-MgCl 2 buffer as the best treatment. V C 2014 International Society for Advancement of Cytometry

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Section: Resultsmentioning

confidence: 99%

“…Several species (e.g., G. eupagioceri ) live in nutritional symbiosis with ambrosia beetles . Geosmithia fungi produce hydroxylated anthraquinones as pigmented secondary metabolites and other undetermined pigments. Secondary metabolites are known to have a large impact on measurements of genome size (propidium iodide (PI) binding to DNA; e.g., 37).…”

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Application of flow cytometry for genome size determination in Geosmithia fungi: A comparison of methods

et al. 2014

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“…Future progress might be expected in the analysis of large biomolecules during the next decade [130]. Although faster chromatography of small molecules could remain possible with a generation of very fine (e.g.…”

Section: Future Perspectives For Column Technologymentioning

confidence: 99%

“…Shell particles are a relatively recent trend in chromatographic separation, but several pharmaceutical-bioanalytical [105][106][107][108][109][110][111][112][113][114][115][116][117][118], food analytical [119][120][121][122][123][124][125][126][127][128][129], environmental [130][131][132][133][134] and multidimensional [135][136][137] separations can be found in the literature. Very recently, new commercially available wide-pore stationary phases demonstrate exceptional efficiency for protein separations [138][139][140].…”

Section: Superficially Porous Particlesmentioning

confidence: 99%

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

Fekete¹,

Perrenoud²,

Guillarme

2014

CCHG

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Abstract:The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. Indeed, better performance in LC has become increasingly important in recent years mainly driven by the challenges of either analyzing more complex samples or increasing the numbers of samples per time unit. In the recent development of particle technology, the use of fully porous sub-2 m particles and sub-3 m shell particles have received considerable attention. Beside packed columns, the new generation of silica-based monolithic columns also offers very high separation power. However, to take full advantage of these innovative phases, the chromatographic system has also to be drastically optimized in terms of upper pressure limit and system volume.This revolution in column technology now spreads and covers several modes of liquid chromatography such as reversedphase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or even supercritical fluid chromatography (SFC). The HILIC and SFC, which can be considered as alternative modes of chromatography, could also be useful to extend the applicability of chromatography towards the analysis of very hydrophilic and lipophilic compounds, respectively.The present review gives an insight about the theory behind the success of current column technology and presents a summary of latest applications, using various modes of one-dimensional chromatography (RPLC, HILIC, SFC). This paper also shows that theoretically expected column efficiency could sometimes be compromised in practical work especially in the case of narrow bore columns.

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“…Three anthraquinones: 1,3,6,8-tetrahydroxy-9,10-anthraquinone; 1-acetyl-2,4,5,7-tetra-hydroxy-9,10-anthraquinone; and 1-acetyl-2,4,5,7,8-pentahydroxy-9,10-anthraquinone were previously isolated from this fungus [3,4]. Also, an analytical HPLC method was reported for the possible separation of another ten new anthraquinones and their proposed structures were determined using only FTMS and UV-VIS spectrometry [3]. In this study, when G. lavendula was cultured in Potato Dextrose Broth (PDB), it produced five anthraquinones ( 1, 2, 3, 5 and 6 ; Figure 1), of which 1-3 were new.…”

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New Anthraquinone Derivatives from Geosmithia lavendula

Malak

Bishay

Abdel-Baky

et al. 2013

Natural Product Communications

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A phytochemical study of Geosmithia lavendula Pitt led to the isolation of three new anthraquinones: 1-acetyl-2,4,6,8-tetrahydroxy-9,10-anthraquinone (1), 2-acetyl-1,4,5,7-tetrahydroxy-9,10-anthraquinone (2), and 1-acetyl-2,4,5,6,7-pentahydroxy-9,10-anthraquinone (3), as well as another new compound named didodecyl thiodipropionate (propionic acid, 3,3-sulfinyl di-1,1′-didodecyl ester) (4), along with ten known compounds: 1-acetyl-2,4,5,7-tetrahydroxy-9,10-anthraquinone (rhodolamprometrin) (5), 1-acetyl-2,4,5,7,8-pentahydroxy-9,10-anthraquinone (6), (22E)-ergosta-6,22-diene-3β,5α,8α-triol, p-hydroxybenzyl alcohol, oleic acid, D-mannitol, palmitic acid, stearic acid, cis-vaccenic acid and 2-decenal. The structures of the isolated metabolites were elucidated based on NMR spectroscopic and mass spectrometric data. Compound 1 exhibited moderate activity against methicillin resistant Staphylococcus aureus with an IC50 value of 16.1 μg/mL.

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High-performance liquid chromatography–off line mass spectrometry analysis of anthraquinones produced by Geosmithia lavendula

Cited by 21 publications

References 28 publications

Application of flow cytometry for genome size determination in Geosmithia fungi: A comparison of methods

Application of flow cytometry for genome size determination in Geosmithia fungi: A comparison of methods

Evolution and Current Trends in Liquid and Supercritical Fluid Chromatography

New Anthraquinone Derivatives from Geosmithia lavendula

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