High performance liquid chromatography of <i>zanhic</i> acid glycoside in alfalfa (<i>Medicago sativa</i>)

Nowacka, J.; Oleszek, Wiesław

doi:10.1002/pca.2800030506

Cited by 21 publications

(16 citation statements)

References 10 publications

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“…These data suggest that within the group of zanhic acid glycosides, the aglycone structure (16 OH derivative of medicagenic acid) is as important as the number of sugar substitutions as a determinant of activity against the mucosal cells. It is noteworthy than the zanhic acid tridesmoside is present in the aerial parts of alfalfa, and in some varieties its concentration may reach 095% of dry matter (Nowacka and Oleszek 1992).…”

Section: Discussionmentioning

confidence: 99%

Effects of some purified alfalfa (Medic ago sativa) saponins on transmural potential difference in mammalian small intestine

et al. 1994

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Abstract:The effect of a range of structurally divergent alfalfa saponins on the potential difference (PD) across the rat small intestine has been examined in uitro. Typically, there was an immediate reduction in PD, although there was considerable variation in response to particular compounds. Among the glycosides of medicagenic acid, bisdesmosides containing four sugar moieties had activities equal to the monodesmoside and to medicagenic acid itself. The exception was 3Glc,28Glc-medicagenic acid, which did not affect the membrane potential. Zanhic acid glycosides reduced the PD to a much greater extent than medicagenic acid derived compounds. The tridesmoside of zanhic acid was the most active compound tested. This compound is however not precipitated by cholesterol and hence would not have been present in the 'biologically active' fractions used in previous studies. The present data show that both the lipophilic aglycone portion of saponins, and the sugar composition can influence the ability of these compounds to depolarise the intestinal mucosa. The observations are discussed in the light of antinutritional properties of alfalfa saponins when fed to monogastric animals.

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Section: Discussionmentioning

confidence: 99%

Effects of some purified alfalfa (Medic ago sativa) saponins on transmural potential difference in mammalian small intestine

et al. 1994

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“…Solvents were filtered through a Millipore filter (0 45 pm) and degassed by sonication prior to use. Gradients used were those described by Nowacka and Oleszek, 1992. Derivatized saponins were eluted at 1 mL/min and detection was by monitoring at 260 nm.…”

Section: Extraction From Rootsmentioning

confidence: 99%

Alfalfa saponins and sapogenins: Isolation and quantification in two different cultivars

Tava

Oleszek

Jurzysta

et al. 1993

Phytochemical Analysis

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The chemical characterization of the saponins and sapogenins isolated from roots and aerial parts of two alfalfa cultivars with differing saponin content is reported. A procedure for the extraction and quantification of saponins is described, and the identification of the major components of the saponin mixture has been performed using thin layer chromatography and high performance liquid Chromatography. Characterization, using gas chromatography (GC) and GC/mass spectral analysis, of sapogenins released following acid hydrolysis allowed the identification of medicagenic acid, hederagenin, soyasapogenols B, C, D, E and F as the major compounds, together with oleanolic acid. Quantitative analysis of the sapogenins in aerial parts and roots of the two cultivars is reported and discussed.

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“…Saponins of roots and aerial parts of Medicago species were extracted as previously reported by Tava et al (1993) and purified according to Timbekova et al (1989). Pure medicagenic acid (Jurzysta, 1982a) and hederagenin (Jurzysta, 1982b) were obtained from M. sativa and bayogenin (Bialy et al, 2004) (Nowacka and Oleszek, 1992). Acid hydrolysis of saponins from M. sativa and M. arabica (tops and roots) and M. arborea (tops) was carried out following the procedure described by Tava et al (1993).…”

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confidence: 99%

Antimicrobial activity of saponins from Medicago sp.: structure‐activity relationship

Avato

Bucci

Tava

et al. 2006

Phytotherapy Research

195

143

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The antimicrobial activity of saponins from Medicago sativa, M. arborea and M. arabica against a selection of medically important yeasts, Gram-positive and -negative bacteria was investigated. Structure-activity growth inhibitory effects of related prosapogenins and sapogenins are also described. Increasing antibiotic activity was observed going from the saponin extracts to the sapogenin samples, suggesting that the sugar moiety is not important for the antimicrobial efficacy. Activity was especially high against Gram-positive bacteria (Bacillus cereus, B. subtilis, Staphylococcus aureus and Enterococcus faecalis) with M. arabica being the species showing a broader spectrum of action. Discrete antifungal activity was also observed, mainly against Saccharomyces cerevisiae. The observed antimicrobial properties of M. sativa and M. arborea were related to the content of medicagenic acid, while hederagenin seems to contribute to the bioactivity of M. arabica total sapogenins.

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High performance liquid chromatography of zanhic acid glycoside in alfalfa (Medicago sativa)

Cited by 21 publications

References 10 publications

Effects of some purified alfalfa (Medic ago sativa) saponins on transmural potential difference in mammalian small intestine

Effects of some purified alfalfa (Medic ago sativa) saponins on transmural potential difference in mammalian small intestine

Alfalfa saponins and sapogenins: Isolation and quantification in two different cultivars

Antimicrobial activity of saponins from Medicago sp.: structure‐activity relationship

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