High‐performance liquid chromatography of proteins on short capillary columns

Mal’tsev, V. G.; Nasledov, D. G.; Trushin, Sergei A.; Tennikova, Tatiana; Виноградова, Л. В.; Volokitina, I. N.; Zgonnik, V. N.; Belen'kii, B.G.

doi:10.1002/jhrc.1240130310

Cited by 15 publications

(7 citation statements)

References 10 publications

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“…In the mid 1980's, Tennikova and Belenkii [23,24] published the idea that a rather short column length is often sufficient for the separation of proteins in the gradient elution mode. The concept of thin separation beds, further developed in cooperation with FrantiÐek vec of the Prague Institute of Macromolecular Chemistry, who used the experience gained with continuous polymeric matrix as chromatographic separation media [16], has led to the successful development of chromatographic media in a novel format -flat discs.…”

Section: Introductionmentioning

confidence: 99%

Polymethacrylate monolithic columns for capillary liquid chromatography

Urban

Jandera²

2008

J of Separation Science

121

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In recent years, continuous separation media have attracted considerable attention because of the advantages they offer over packed columns. This research resulted in two useful monolithic material types, the first based on modified silica gel and the second on organic polymers. This work attempts to review advances in the development, characterization, and applications of monolithic columns based on synthetic polymers in capillary chromatography, with the main focus on monolithic beds prepared from methacrylate-ester based monomers. The polymerization conditions used in the production of polymethacrylate monolithic capillary columns are surveyed, with attention being paid to the concentrations of monomers, porogen solvents, and polymerization initiators as the system variables used to control the porous and hydrodynamic properties of the monolithic media. The simplicity of their preparation as well as the possibilities of controlling of their porous properties and surface chemistries are the main benefits of the polymer monolithic capillary columns in comparison to capillary columns packed with particulate materials. The application areas considered in this review concern mainly separations in reversed-phase chromatography, ion-exchange chromatography, hydrophobic and hydrophilic interaction modes; enzyme immobilization and sample preparation in the capillary chromatography format are also addressed.

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Section: Introductionmentioning

confidence: 99%

Polymethacrylate monolithic columns for capillary liquid chromatography

Urban

Jandera²

2008

J of Separation Science

121

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“…Porous monolithic polymeric materials have first been reported in the early 1990s. − They were originally designed for high-speed chromatographic separations, nevertheless, their most favorable properties also paved the way for use in other applications, e.g., as catalytic supports in bioreactors . Monolithic polymeric materials (“monoliths”) are characterized by a unitary porous structure with interconnected large pores and are usually synthesized within the confines of the compartment in which they are to be used at a later stage.…”

Section: Introductionmentioning

confidence: 99%

Self-Diffusion of Polystyrene Solutions in Porous Acrylate-Based Monoliths Studied by ¹H PFG NMR

et al. 2010

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The pulsed field gradient (PFG) nuclear magnetic resonance (NMR) method has been used to study the molecular self-diffusion of polystyrene (PS) solutions in porous acrylate-based monoliths. It was found that the characteristic concentration and molar mass dependencies of the PS self-diffusion in the solution (describable by the Zimm and Doi−Edwards models) remain unchanged in case that the self-diffusion occurred in this porous material. However, the self-diffusion coefficients of the PS were reduced in the monoliths by a constant factor, which is independent of the molar mass and the concentration. This constant factor represents the tortuosity of the porous monoliths and agrees with the reduction of the initial turnover frequency found in the ring-closing metathesis reaction of diethyl diallyl malonate catalyzed in these monoliths.

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“…However, the preparation of efficient thin bed layers from particles was too difficult. Although his theory was published a few years later [15], personal discussions we were having already in the mid 1980s stimulated my interest. The boost to the development of the early rigid monoliths came with Tatiana Tennikova who joined my group in Prague.…”

Section: Adagio: Historymentioning

confidence: 98%

My favorite materials: Porous polymer monoliths

Švec¹

2008

J of Separation Science

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High‐performance liquid chromatography of proteins on short capillary columns

Cited by 15 publications

References 10 publications

Polymethacrylate monolithic columns for capillary liquid chromatography

Polymethacrylate monolithic columns for capillary liquid chromatography

Self-Diffusion of Polystyrene Solutions in Porous Acrylate-Based Monoliths Studied by ¹H PFG NMR

My favorite materials: Porous polymer monoliths

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High‐performance liquid chromatography of proteins on short capillary columns

Cited by 15 publications

References 10 publications

Polymethacrylate monolithic columns for capillary liquid chromatography

Polymethacrylate monolithic columns for capillary liquid chromatography

Self-Diffusion of Polystyrene Solutions in Porous Acrylate-Based Monoliths Studied by 1H PFG NMR

My favorite materials: Porous polymer monoliths

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Product

Resources

About

Self-Diffusion of Polystyrene Solutions in Porous Acrylate-Based Monoliths Studied by ¹H PFG NMR