High-performance liquid chromatography of p-nitrophenacyl esters of selected prostaglandins on silver ion-loaded microparticulate cation-exchange resin

Kinetics of epimerization of 15(R)-methylprostaglandin E2 and of 15(S)-methylprostaglandin E2 as a function of pH and temperature in aqueous solution

“…ion loaded microparticulate cation exchange resin column. 13 The p-nitrophenacyl moiety yielded adequate sensitivity for UV detection for this study. The high resolving power of the silver ion loaded column allowed the separation not only of the ester derivatives of 15-methylprostaglandin E2's from one another but also from other possible reaction products (see below).…”

“…The preparation of this column from a prepacked 10-µ Partisil SCX (Reeve Angel) column has been previously described. 13 The column was thermostated at 26 ± 0.1 °C using a Haake Model FJ circulating, constant-temperature bath and a homemade stainless steel column jacket. A 10-µ Precision Sampling Pressure-Lok syringe was used for sample injection.…”

High Performance Liquid Chromatography in the Analysis of Prostaglandins, Thromboxanes and Leukotrienes

“…Another method for the separation of prostaglandins is argentation HPLC. Merritt and Bronson (1977) prepared a silver ion-loaded microparticulate cationexchange resin column for HPLC separation of the p-nitrophenacyl esters of several series of closely related prostaglandins, eluted with dioxane/acetonitrile (99.94/0.06 v/v). Powell (1981) extended the study to radioactive, underivatized prostaglandins on a cation exchange column treated with silver nitrate solution.…”

Ion exchange and liquid column chromatography

“…Silica gel, loaded with cadmium by a hydrothermal treatment, was also used to separate aromatic amines (486). Silver-loaded silica gels (7, 177, 268, 290) and ion-exchange resins (313,314,471) separated cis-trans isomers of various compounds as well as electron-donor aromatic compounds in general: see Table II. A variant on this procedure is to use thallium (I), which binds ligands less strongly than silver (I) but reacts much more rapidly, giving better column efficiencies (427).…”