High-Performance Liquid Chromatography of Lignin-Derived Phenols in Environmental Samples with Diode Array Detection

Lobbes, J. M.; Fitznar, H. P.; Kattner, Gerhard

doi:10.1021/ac981175i

Cited by 66 publications

(66 citation statements)

References 26 publications

(57 reference statements)

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“…All samples were analyzed in duplicate, including CuO oxidation, extraction, and quantification. The coefficient of variation (CV) was 15% on average that agrees well with data reported elsewhere (Lobbes et al 1999). The CV of the HPLC quantification was <3%.…”

Section: Sampling and Analysissupporting

confidence: 90%

“…All international standards were obtained from the National Bureau of Standards in Gaithersburg (MD, USA). Lignin was analyzed by disrupting its macromolecular structure by alkaline CuO oxidation (Hedges and Ertel 1982;Lobbes et al 1999). A suite of phenolic monomers derived by this method was analyzed by a high performance liquid chromatography.…”

Section: Sampling and Analysismentioning

confidence: 99%

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Sources and dynamics of sedimentary organic matter in Sundarban mangrove estuary from Indo-Gangetic delta

et al. 2017

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Introduction: Mangrove forests are highly productive ecosystems distributed along the tropical coast line. Nutrient biogeochemistry of mangroves are primarly driven by both allochthonous and autochthonous sources. Characterization of organic matter in coastal ecosystems enables to understand the biogeochemical transformation of organic matter and its influence on ecosystem productivity in response to various changing environmental conditions. Therefore, the elemental and stable carbon and nitrogen levels were employed to understand the organic matter (OM) dynamics in the Sundarban mangrove ecosystem (India-Bangladesh). Results: Differences in stable isotope values indicate that variable sources influence the OM dynamics in Sundarban sediments. The progressive enrichment in δ 13C levels along the land-coastal continuum indicates that the terrestrial and marine inputs are dominant at the landward and seaward ends, respectively. The CuO oxidationderived lignin phenol monomers describe significant levels of total lignin are preserved in Sundarban mangrove sediments during diagenesis. The phenol monomer ratios are lower than the plant litter explaining that aromatic ring cleavage is the dominant mechanism for the lignin degradation. Furthermore, the Ad/Al ratios were higher than the plant litter explaining the oxidation of propyl side chain of vascular OM influencing the carbon cycling in Sundarban sediments. Largely, the Ad/Al ratios describe the vascular OM degradation is through the oxidation of propyl side chain.

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Section: Sampling and Analysissupporting

confidence: 90%

Section: Sampling and Analysismentioning

confidence: 99%

Sources and dynamics of sedimentary organic matter in Sundarban mangrove estuary from Indo-Gangetic delta

et al. 2017

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“…Various separation (mainly high performance liquid chromatography) and optical techniques have been exploited to eliminate the interferences caused by other components (Lobbes et al 1999).…”

Section: Introductionmentioning

confidence: 99%

UV/Vis Spectrometry as a Quantification Tool for Lignin Solubilized in Deep Eutectic Solvents

et al. 2017

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In this short communication, UV/Vis spectrophotometry is described as an analytical tool for the quantification of lignin content in deep eutectic solutions. The lignin was solubilized with different deep eutectic solvent (DES). DESs were prepared as binary mixtures of choline chloride with lactic acid (1:9); (1:10); ethylene glycol (1:2); glycerol (1:2) and alanine:lactic acid (1:9), and betaine:lactic acid (1:2). The UV-Vis spectrometric quantification of the solubilized lignins was independent of the type of solubilized lignin. The approach consists of measuring the absorbance of a solution of lignins dissolved in the deep eutectic solvents at an absorbance of 440 nm.

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“…17 Without the need of derivatization, high-performance liquid chromatography (HPLC) was used to the analysis of these compounds. 18,19 CE is also a non-derivatization method and has been rapidly developing as an alternative separation method to traditional HPLC. This is predominantly due to the fact that CE is based on an electro-driven separation mechanism and therefore possesses the superior resolving power, resulting in a reduction in analysis time.…”

Section: Introductionmentioning

confidence: 99%

Capillary Electrophoretic Determination of Selected Phenolic Compounds in Humic Substances of Well Waters and Fertilizers

Chen

Chang

et al. 2010

ANAL. SCI.

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In this study, nine oxidized phenolics, which were often found in HS and provide useful information on the taxonomic source and diagenetic state of HS, gallic acid and protocatechuic acid, were employed standard solution to set up the CZE system 2010 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. Humic substances (HS) from well waters, fertilizers, and synthetic phenolic polymers were characterized by elemental and UV-VIS spectroscopic analyses. Capillary zone electrophoresis (CZE) with UV absorption detection was used to analyze the lignin-derived phenolic distribution in the degradation residues after alkaline CuO oxidation of HS samples. Eleven phenols with p-acetyl, vanillyl and syringyl substituents were selected to optimize the CZE parameters. For well waters and fertilizers, the content of phenolic fragments was in agreement with the findings of the elemental and spectroscopic measurements.Additionally, parameters derived from the vanillic acid/vanilline, syringyl acid/syringaldehyde, p-hydroxyl/vanillyl and syringyl/vanillyl ratios matched analogous studies on dissolved organic matter from natural waters and on humic acids from terrestrial substances. The amount of phenolic monomer bonded within two synthetic HS polymers was found to be 25.9% protocatechuic acid and 71.3% gallic acid.

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High-Performance Liquid Chromatography of Lignin-Derived Phenols in Environmental Samples with Diode Array Detection

Cited by 66 publications

References 26 publications

Sources and dynamics of sedimentary organic matter in Sundarban mangrove estuary from Indo-Gangetic delta

Sources and dynamics of sedimentary organic matter in Sundarban mangrove estuary from Indo-Gangetic delta

UV/Vis Spectrometry as a Quantification Tool for Lignin Solubilized in Deep Eutectic Solvents

Capillary Electrophoretic Determination of Selected Phenolic Compounds in Humic Substances of Well Waters and Fertilizers

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