High-performance liquid chromatography method for the simultaneous determination of thiamine hydrochloride, pyridoxine hydrochloride and cyanocobalamin in pharmaceutical formulations using coulometric electrochemical and ultraviolet detection

Marszałł, Michał Piotr; Lebiedzińska, Anna; Czarnowski, Wojciech; Szefer, Piotr

doi:10.1016/j.chroma.2005.07.091

Cited by 79 publications

(27 citation statements)

References 41 publications

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“…The chemical structure of all the particular vitamin B 6 forms enables their measurement by electrochemical detection at nanomolar concentrations in endogenous plasma and serum. It should be mentioned that use of electrochemical detection has been already reported in literature [44][45][46][47][48]. However, such application was limited to samples other than pharmaceuticals and foods.…”

Section: Introductionmentioning

confidence: 97%

Application of the high-performance liquid chromatography method with coulometric detection for determination of vitamin B6 in human plasma and serum

Marszałł

Lebiedzińska

Czarnowski

et al. 2009

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 97%

Application of the high-performance liquid chromatography method with coulometric detection for determination of vitamin B6 in human plasma and serum

Marszałł

Lebiedzińska

Czarnowski

et al. 2009

Journal of Chromatography B

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“…There is still great interest in developing new methods in HPLC to simultaneously determine a larger number of possible vitamins [31,32]. A very good alternative to HPLC is CE because of its better resolution and, generally, shorter analysis time [30,33,34].…”

Section: Introductionmentioning

confidence: 99%

“…Excellent separations have been achieved using CZE [35,36] and MEKC [37][38][39] in vitamin supplements. However, only two of the papers mentioned above [32,39] used ED to determine pyridoxine (B6), cyanocobalamin (B12), nicotinamide (VPP), and ascorbic acid (C). ED of water-soluble vitamins in pharmaceutical preparations and food has been performed with good results for B6, B12, C and biotin (H) [40][41][42][43][44].…”

Section: Introductionmentioning

confidence: 99%

A fast and reliable route integrating calibration and analysis protocols for water‐soluble vitamin determination on microchip‐electrochemistry platforms

et al. 2006

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A novel analytical route to determine water-soluble vitamins (B group and C) using single channel microchip-electrochemistry platforms is presented. The electrochemical detection protocol was carefully optimized, and it was shown that it was crucial to use 1 M nitric acid in the detector compartment to detect folic acid. A phosphate buffer (pH 6, 10 mM) and a separation voltage of 2 kV gave the complete separation of vitamins in less than 130 s, with good reproducibility (RSDs less than 10%) and accuracy (error less than 9%). In addition, a methodological innovation integrating calibration and analysis of water-soluble vitamins on the chip is also proposed. The strategy consisted in sequentially using both reservoirs (named calibration and analysis reservoirs) as well as a calibration factor (defined as signal/concentration of analyte). The analytical route required 350 s in the overall protocol (employing 130 s in calibration plus 130 s in analysis), an improvement over the times used in both conventional and microchip protocols.

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“…The HPLC coupled with electrochemical detector (HPLC-ED) is believed to be sensitive and selective method for the determination of redox compounds. It is one of the analytical methods that has been recommended for the determination of B-group vitamins in pharmaceutical formulations and biological matrices (Ekinci, 2005;Marszall et al, 2005;Mimica et al, 2002). For analysis of seafood (fish, mussels, oysters) and food products of plant origin (apple juices and fortified fruit juices), Lebiedzinska et al (2007) have reported an HPLC method with ED and UV detection for the simultaneous determination of vitamins B1, B6, and B12.…”

Section: Simultaneous Determination Of Water-soluble Vitaminsmentioning

confidence: 99%

Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

Abano

Dadzie

2014

Croat. J. Food Sci. Technol.

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SummaryThe water-soluble vitamins (WSV): ascorbic acid (vitamin C), thiamine (B1), riboflavin (B2), niacin (B3), panthothenic acid (B5), pyridoxine, and pyridoxal (B6), folic acid (B9), biotin(B8) , and B12 are very essential in the diet of humankind. As a result of ever increasing pressures from both consumers and legal enforcers, to specify accurately nutritive compositions of WSV that are present in food materials, many researchers have attempted to fill this niche through the provision of highly sensitive and rapid high performance liquid chromatography (HPLC) procedures. In view of the health benefits of WSV, a replete of HPLC methods have been developed for simultaneous determination of their contents in nature and fortified food samples, nutritional supplements, as well as blood plasmas. The rate of losses of these vitamins during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive HPLC procedure for their simultaneous separations and assays. This review critically assesses the different HPLC procedures developed by researchers and available in the open literature for simultaneous determination of water-soluble vitamins (WSV) in dried tropical fruits materials. The study revealed that not a single chromatographic run developed by researchers can simultaneously elute all the WSV at a time. However, the HPLC procedures that are capable of determining all the WSV were coupled with electrospray ionization mass spectroscopy (ESI-MS), thus making the set-up expensive.

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High-performance liquid chromatography method for the simultaneous determination of thiamine hydrochloride, pyridoxine hydrochloride and cyanocobalamin in pharmaceutical formulations using coulometric electrochemical and ultraviolet detection

Cited by 79 publications

References 41 publications

Application of the high-performance liquid chromatography method with coulometric detection for determination of vitamin B6 in human plasma and serum

Application of the high-performance liquid chromatography method with coulometric detection for determination of vitamin B6 in human plasma and serum

A fast and reliable route integrating calibration and analysis protocols for water‐soluble vitamin determination on microchip‐electrochemistry platforms

Simultaneous detection of water-soluble vitamins using the High Performance Liquid Chromatography (HPLC) - a review

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