High-performance liquid chromatography method for simultaneous determination of aliphatic acid, aromatic acid and neutral degradation products in biomass pretreatment hydrolysates

Chen, Shou‐Feng; Mowery, Richard A.; Castleberry, Vanessa A.; Walsum, G. Peter van; Chambliss, C. Kevin

doi:10.1016/j.chroma.2005.11.136

Cited by 105 publications

(74 citation statements)

References 41 publications

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“…Methylmalonic acid (MMA) 3 in serum/plasma or urine was introduced as a marker of cobalamin (vitamin B 12 ) deficiency more than 20 years ago (1)(2)(3)(4)(5)(6). The different methods that have been developed for measuring MMA in serum, plasma, urine, and cerebrospinal fluid include GC-MS, liquid chromatographytandem mass spectrometry (LC-MS/MS), HPLC, and capillary electrophoresis (7)(8)(9)(10).…”

confidence: 99%

Quantification of Methylmalonic Acid in Human Plasma with Hydrophilic Interaction Liquid Chromatography Separation and Mass Spectrometric Detection

Lakso

Appelblad²,

Schneede

2008

Clinical Chemistry

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confidence: 99%

Quantification of Methylmalonic Acid in Human Plasma with Hydrophilic Interaction Liquid Chromatography Separation and Mass Spectrometric Detection

Lakso

Appelblad²,

Schneede

2008

Clinical Chemistry

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“…Note that data in Figure 1 were collected over 2 min. Compared with traditional chromatographic separations of biomass hydrolysates [6,10,13,45], a 2-min analysis time represents a 10-to 20-fold increase in throughput. In addition, these data exhibit an increase in peak capacity from~79,000 with HRMS-only analysis to~350,000 with IM-HRMS.…”

Section: Characterization Of Im-ms Spectra Of Biomass Pretreatment Hymentioning

confidence: 99%

“…Accordingly, detailed characterization of potential biofuel process streams is a critical step in optimizing biomass-to-fuel conversions, which are required for viable energy yields [1]. Most current biomass analyses target bulk components [2][3][4][5] or screen only a small fraction [6][7][8][9][10][11][12][13] of the total molecular composition. Moreover, these techniques can involve timeconsuming preparation steps, extended analysis times, and multiple analyses for different compound classes [6][7][8][10][11][12][13][14].…”

mentioning

confidence: 99%

“…IM-MS correlations for structural carbohydrate oligomers in a bio-matrix have also been established to aid rapid identification of potential carbohydrates in a sample. While other techniques involve labor-intensive sample clean-up steps (e.g., multiple liquid and/or solid phase extractions [6]) or time-consuming chromatographic separation prior to MS analysis [6,7,13], DIESI-IM-HRMS is demonstrated to offer a high-throughput approach for rapid screening of whole-sample hydrolysates.…”

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confidence: 99%

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Direct Infusion Electrospray Ionization – Ion Mobility – High Resolution Mass Spectrometry (DIESI-IM-HRMS) for Rapid Characterization of Potential Bioprocess Streams

Munisamy

Chambliss

Becker

2012

J. Am. Soc. Mass Spectrom.

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Direct infusion electrospray ionization Yion mobilityYhigh resolution mass spectrometry (DIESI-IM-HRMS) has been utilized as a rapid technique for the characterization of total molecular composition in "whole-sample" biomass hydrolysates and extracts. IM-HRMS data reveal a broad molecular weight distribution of sample components (up to 1100 m/z) and provide trendline isolation of feedstock components from those introduced "in process." Chemical formulas were obtained from HRMS exact mass measurements (with typical mass error less than 5 ppm) and were consistent with structural carbohydrates and other lignocellulosic degradation products. Analyte assignments are supported via IM-MS collision-cross-section measurements and trendline analysis (e.g., all carbohydrate oligomers identified in a corn stover hydrolysate were found to fall within 6 % of an average trendline). These data represent the first report of collision cross sections for several negatively charged carbohydrates and other acidic species occurring natively in biomass hydrolysates.

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“…[5][6][7][8][9][10] For the anion analysis, ion chromatography (IC) has its particular predominance and has gradually developed into the preferred method for the determination of anions since it was introduced in 1975. For the determination of weak organic acid, ion exclusion chromatography rather than ion-exchange chromatography was often selected as an analytical method.…”

Section: Introductionmentioning

confidence: 99%

Study on the Retention Behavior of Aromatic Carboxylic and Sulfonic acid on a New Anion Exchange Column

2008

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Ion chromatography (IC) has gradually developed into a preferred method for the determination of inorganic anions. And in recent years some low molecular aliphatic acid can be also separated in the ion exchange column with the development of stationary phase. But for the determination of aromatic ionic compounds there are some problems. The aromatic anions show enhanced retention due to interaction with the π electrons of the aromatic backbone. Although the addition of an organic modifier can alleviate the difficulty, it is not the ultimate solution. IonPac AS20 column was developed using a unique polymer bonding technology and its substrate coating is aliphatic backbone. The polymer is completely free of any π electron-containing substituents in the AS20 column. In this paper, the retention behavior of aromatic carboxylic and sulfonic acid on two hydroxide-selective columns, IonPac AS11-HC, AS16, and the new column AS20 was also studied. The result showed that the retentions of ten compounds on three columns were different with each other because of their different column characteristics. Among them 4-chlorobenzene sulfonic acid, 3,5-dihydric benzoic acid and salicylic acid obviously exhibited the weakest retention on the IonPac AS20. It was showed that π-π bond function between anion and stationary phases was weakened in AS20 column because its polymer was completely free of any π electron-containing substituents. So in this paper the AS20 was selected as an analytical column to separate ten aromatic ionic compounds, fumaric acid with conjugate bond included. The retention behavior, separation of the ten compounds and effect of temperature on their retention in the anion-exchange column AS20 (2 mm) were studied. The result showed that those compounds could be separated with each other when running in gradient program and the organic modifier was unnecessary during the separation. So it is showed that AS20 column can be used as a separating column because its polymer is completely free of any π electron-containing substituents. Finally, the effect of temperature on the retention behavior in AS20 column was studied and it was showed that the retention of nine compounds exhibited endothermic behavior.

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High-performance liquid chromatography method for simultaneous determination of aliphatic acid, aromatic acid and neutral degradation products in biomass pretreatment hydrolysates

Cited by 105 publications

References 41 publications

Quantification of Methylmalonic Acid in Human Plasma with Hydrophilic Interaction Liquid Chromatography Separation and Mass Spectrometric Detection

Quantification of Methylmalonic Acid in Human Plasma with Hydrophilic Interaction Liquid Chromatography Separation and Mass Spectrometric Detection

Direct Infusion Electrospray Ionization – Ion Mobility – High Resolution Mass Spectrometry (DIESI-IM-HRMS) for Rapid Characterization of Potential Bioprocess Streams

Study on the Retention Behavior of Aromatic Carboxylic and Sulfonic acid on a New Anion Exchange Column

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