HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD VALIDATION OF α-MANGOSTIN ASSAY IN MANGOSTEEN (Garcinia mangostana L.) FRUIT RIND EXTRACT FORMULATED IN ORAL SOLUTION

Abstract

<p>Mangosteen fruit rind extract contain a lot of antioxidants. α-Mangostin is a component in mangosteen fruit rind that has highest antioxidant effect. The oral solution containing mangosteen fruit rind extract is required an assay method for quality assessment. Determination of a very low concentration of analyte in sample with very complex matrix, such as α-mangostin in oral solution, needs a selective and sensitive method, such as high performance liquid chromatography (HPLC). In this study, α-mangostin assay was performed by reverse phase HPLC system using octadecylsilane (C18) as stationary phase, methanol-water (90:10) as mobile phase, the flow rate is 1.0 mL/min, and the UV detector at 316 nm. The retention time of α-mangostin was 9.622 minutes. Peak of α-mangostin was well separated with resolution of 1.725. Linearity was in the range of 1.67-5.01 ppm with correlation coefficient of 0.9986. The relative standard deviation (RSD) was 1.30 %, the recovery was in the range of 95.80-100.76 </p>

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD VALIDATION OF α-MANGOSTIN ASSAY IN MANGOSTEEN (Garcinia mangostana L.) FRUIT RIND EXTRACT FORMULATED IN ORAL SOLUTION

“…KCKT telah banyak digunakan untuk menetapkan kadar α-mangostin di dalam ekstrak dari kulit buah manggis (Aisha et al, 2013;Yodhnu et al, 2009;Pothitirat and Gritsanapan, 2009;Islam and Begum, 2011;Jujun et al, 2009), ekstrak yang dienkapsulasi dalam PLGA (Poly Lactic-co-Glycolic Acid) microspheres (Ali et al, 2012), dan α-mangostin dalam plasma darah tikus (Syamsudin et al, 2010). Kondisi optimum KCKT untuk menetapkan kadar α-mangostin dalam larutan oral ekstrak kulit buah manggis dicapai menggunakan oktadesilsilan (C18) sebagai fase diam, metanol-air (90:10) sebagai fase gerak dengan laju alir 1 mL/menit dan detektor UV 316 nm (Nurhidayati et al, 2014).…”