High-performance liquid chromatography (HPLC) Identification of five new phenolic compounds involved in the olive tree (Olea europea var. Sigoise) resistance to Verticillium dahliae

Abstract

Verticillium wilt is a vascular disease caused by Verticillium dahliae which represents a serious threat for olive growing in Algeria. Many studies have shown the potential involvement of phenolic compounds in the reaction of plants to pathogens. Our study shows that the presence of Verticillium wilt induces a high production of polyphenols in infected olive trees compared to uninfected ones. The presence of high concentrations of flavonoids (3.45%) and alkaloids (0.44%) in the infected trees suggests that flavonoids and alkaloids may play a role in the olive tree resistance to verticillium wilt. The high performance liquid chromatography (HPLC) analysis showed the presence of five phenolic compounds: oleuropeine, luteonine, catechin, and for the first time verbascoside, apigenine-7-glycoside and some derivatives hydroxycinnamic compounds. These substances are good resistance markers and should help to make efficient strategies for the biocontrol of verticillium wilt.

The role of phenolic compounds in the defense of sooty mold of olive leaves (Olea europea L.)

“…These results are similar to that found by Bensalah et al (2014) who found olive infected by V. dahliae.…”

“…Bensalah et al (2014) found verbascoside for the first time in olive leaves infected by V. dahliae. This result confirms that verbascoside compounds have a role in the resistance or defense of olive against fungi attacks.…”