High performance liquid chromatography-electrospray ionization mass spectrometric determination of tolterodine tartrate in human plasma

Zhang, Beibei; Zhang, Zunjian; Tian, Yuan; Xu, Fengguo

doi:10.1016/j.jchromb.2005.07.010

Cited by 23 publications

(16 citation statements)

References 8 publications

(8 reference statements)

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“…Liquid chromatographyelectrospray mass spectrometry (LC-ESI-MS) has emerged as a sensitive analytical technique for the determination of various compounds (Grace et al, 2003;Bogialli et al, 2003;Vernez et al, 2003;Zhang et al, 2005;Fuh et al, 2004). The aim of this investigation is to develop a LC-ESI-MS method incorporated with microdialysis technique to determine sinomenine and study the pharmacokinetics of free-form sinomenine in rat skin.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic study of free‐form sinomenine in rat skin by microdialysis coupled with liquid chromatography–electrospray mass spectrometry

Zheng

Shi

Jin-hong

2006

Biomedical Chromatography

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Sinomenine (7,8-didehydro-4-hydroxy-3,7-dimethoxy-17-methylmorphinan-6-one) is a pure alkaloid extracted from the Chinese medical plant. In this report a liquid chromatography-electrospray mass spectrometry (LC-ESI-MS) method with in vivo microdialysis for the pharmacokinetic study of free-form sinomenine in rat skin has been developed. A microdialysis probe was surgically implanted into the subcutaneous tissue of the rats and an isotonic phosphate buffer (PBS) was used as the perfusion medium. Samples were collected and then analyzed off-line by LC-ESI-MS. The chromatographic separation was achieved within 4.2 min by using a narrow-bore Xterra C(18) column (2.1 x 150 mm, 5 microm) with acetonitrile-(10 mmol/L ammonium acetate buffer, 0.1% acetic acid) (15:85, v/v). Ion signal m/z 330.1 for sinomenine was measured in the positive mode. Linearity was established for the range of concentrations of 2.0-10000.0 ng/mL with a coefficient of determination (r) of 0.9989. The intra- and inter-day reproducibility of the present method was better than 6%. The lower limit of quantification (LLOQ) was 1.0 ng/mL. The proposed method described provides more authentic information on pharmacokinetics and metabolism at the site of action by using the coupling of microdialysis to LC-ESI-MS technique than the traditional sampling methods.

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Section: Introductionmentioning

confidence: 99%

Pharmacokinetic study of free‐form sinomenine in rat skin by microdialysis coupled with liquid chromatography–electrospray mass spectrometry

Zheng

Shi

Jin-hong

2006

Biomedical Chromatography

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“…n-Hexane-isopropanol (95:5, v/v) used for the extraction of tolterodine [4] had lower recovery for the metabolite (only about 30% compared to the detector response obtained for the true concentration of the pure authentic standard).…”

Section: Sample Preparationmentioning

confidence: 99%

“…A GC/MS method [3] was not sufficiently sensitive to follow the pharmacokinetics of tolterodine after therapeutic doses. The LC/MS assay reported by Zhang et al [4] had lower limit of quantitation (0.1 ng/ml) but was developed only for determination of the parent compound and thus is not suitable for measuring the sum of concentrations of tolterodine and 5-hydroxymethyl metabolite which is important for the therapeutic response.…”

Section: Introductionmentioning

confidence: 99%

Determination of tolterodine and its 5-hydroxymethyl metabolite in human plasma by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Máček¹,

Ptáček²,

Klima³

2009

Journal of Chromatography B

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“…These methods are developed by spectrophotometry, high performance liquid chromatography, gas chromatography-mass spectrometry, and capillary electrophoresis techniques (9)(10)(11)(12). The techniques require sophisticated instruments or rigorous sample preparation procedures.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Method for the Determination of Tolterodine In Human Plasma and Pharmaceutical Preparations by Derivatization with Dansyl Chloride

Tekkeli¹

2017

Bezmialem Science

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Objective: Tolterodine (TOL) is an anti-muscarinic drug used for the symptomatic treatment of urinary incontinence. Analytical methods in the literature are not sensitive enough for the assay of drug substance biological fluids. To make up the deficit, we developed a sensitive, costreduced spectrofluorimetric method for the determination of TOL in plasma and pharmaceutical preparations.Methods: TOL has a phenolic hydroxyl group that reacts with dansyl chloride. The spectrofluorimetric method is based on this reaction in the presence of a bicarbonate solution of pH 9.5 to give a highly fluorescent derivative, which can be measured at 590 nm with an excitation wavelength at 360 nm in dichloromethane. Different experimental parameters affecting fluorescence intensity were optimized. The relationship between fluorescence intensity and TOL concentration was investigated. Results:The developed method is linear in the concentration range of 5-60 ng mL −1 with a minimum detectability of 0.136 ng mL −1 . The recovery was 100.48%. Conclusion:A simple, cost-effective, and sensitive spectrofluorimetric method was developed and validated for the determination of TOL in human plasma and pharmaceutical preparations using dansyl chloride. According to this validation study, it is possible to use this proposed method for the routine analysis of the drug and for conducting bioavailability and bioequivalence studies with good accuracy and precision.

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High performance liquid chromatography-electrospray ionization mass spectrometric determination of tolterodine tartrate in human plasma

Cited by 23 publications

References 8 publications

Pharmacokinetic study of free‐form sinomenine in rat skin by microdialysis coupled with liquid chromatography–electrospray mass spectrometry

Pharmacokinetic study of free‐form sinomenine in rat skin by microdialysis coupled with liquid chromatography–electrospray mass spectrometry

Determination of tolterodine and its 5-hydroxymethyl metabolite in human plasma by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Spectrofluorimetric Method for the Determination of Tolterodine In Human Plasma and Pharmaceutical Preparations by Derivatization with Dansyl Chloride

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