High performance liquid chromatography-based method to analyze activity of GABA transporters in central nervous system

Moraes, Edinaldo Rogério da Silva; Santos-Silva, Mateus; Grisólia, Alan Araújo; Braga, Danielle Valente; Leão, Luana Ketlen Reis; Bahia, Carlomagno Pachecco; Moraes, Suellen Alessandra Soares de; Passos, Adelaide Fonseca; Batista, Evander de Jesus Oliveira; Herculano, Anderson Manoel; Oliveira, Karen Renata Matos

doi:10.1016/j.neuint.2022.105359

Cited by 2 publications

(2 citation statements)

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“…The samples to be tested were prepared by pre-column derivatization using the reference amino acid analysis system (Dalian Elite) [23]. An Agilent ZORBAX SB-Aq (250 mm × 4.6 mm, 5 μm) column was used and maintained at 30 °C.…”

Section: Determination Of Gabamentioning

confidence: 99%

“…In the detection range, the peak area of each ingredient showed a linear relationship with the concentration (r > 0.9991); the repeatability RSD (n = 6) was < 2.67%; the precision RSD (n = 6) was < 2.22%; the stability was good within 24 h, and the stability RSD (n = 6) was < 2.56%; the recoveries of spiked samples were in the range of 95.3% ~ 103.2%, and the RSD (n = 6) was less than 7.27%. The results showed that the established quantitative method is accurate, reliable, and meets the relevant requirements so that it can be used for the determination of the ingredients of black ginseng [17][18][19][20][21][22][23][24].…”

Section: Determination Of Chemical Compositionmentioning

confidence: 99%

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Anti-prostate cancer mechanism of black ginseng during the "nine steaming and nine sun-drying" process based on HPLC analysis combined with vector space network pharmacology

Zhang,

Huang,

Dou

2024

Discov Onc

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HPLC analysis determined six small-molecule organic acids, maltol, 5-hydroxymethylfurfural (5-HMF), 17 ginsenosides, four oligosaccharides, and 20 amino acids in black ginseng samples with different processing times. Based on the content determination results, the differential ingredients in the processing of black ginseng were screened by multivariate statistical analysis. Network pharmacological methods obtained the core targets and pathways of the above ingredients against prostate cancer. Finally, the entropy weight method was used to assign values to the above ingredients, targets, and pathways, and the vector space network pharmacology method was established to study the anti-prostate cancer mechanism of black ginseng in the process of "nine steaming and nine sun-drying". Based on principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), fructose, glucose, dencichin, glutamate, ginsenoside 20 (S)-Rg3, 20 (R)-Rg3, 20 (S)-Rh2, Rg1, Re, and Rc were the main differential ingredients in various steaming and sun-drying cycle periods of black ginseng. The results of vector space network pharmacology showed that the main reason for the change in the anti-prostate cancer pathway of black ginseng with the number of steaming and sun-drying was the different regulatory ability of black ginseng on the PI3K-Akt signaling pathway and chemical carcinogenesis-receptor activation pathway. It gave researchers a fresh perspective for exploring the anti-prostate cancer active components of black ginseng and the change in the mechanism of the effect of traditional Chinese medicine in processing.

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Section: Determination Of Gabamentioning

confidence: 99%

Section: Determination Of Chemical Compositionmentioning

confidence: 99%

Anti-prostate cancer mechanism of black ginseng during the "nine steaming and nine sun-drying" process based on HPLC analysis combined with vector space network pharmacology

Zhang,

Huang,

Dou

2024

Discov Onc

View full text Add to dashboard Cite

show abstract

Anti-prostate cancer mechanism of black ginseng during the "Nine Steaming and Nine Sun-Drying" process based on HPLC analysis combined with vector space network pharmacology

Zhang,

Huang,

Dou

2023

Preprint

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Purpose The vector space network pharmacology method was established to investigate the anti-prostate cancer mechanism of black ginseng in the process of "nine steaming and nine sun-drying". Methods Six small-molecule organic acids, maltol, 5-HMF, 17 ginsenosides, 4 oligosaccharides, and 20 amino acids in black ginseng samples with different processing times were determined by HPLC analysis. Based on the content determination results, the differential ingredients in the processing of black ginseng were screened by multivariate statistical analysis. The core targets and pathways of the above ingredients against prostate cancer were obtained by network pharmacological methods. Finally, the entropy weight method was used to assign values to the above ingredients, targets, and pathways, and the vector space network pharmacology method was established to study the anti-prostate cancer mechanism of black ginseng in the process of "nine steaming and nine sun-drying". Results Based on principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), fructose, glucose, dencichin, glutamate, ginsenoside 20 (S)-Rg3, 20 (R)-Rg3, 20 (S)-Rh2, Rg1, Re, and Rc were the main differential ingredients in various steaming and sun-drying cycle periods of black ginseng. The results of vector space network pharmacology showed that the main reason for the change in the anti-prostate cancer pathway of black ginseng with the number of steaming and sun-drying was the different regulatory ability of black ginseng on the PI3K-Akt signaling pathway and chemical carcinogenesis-receptor activation pathway. Conclusion The study found that the content of each ingredient in black ginseng changed with the number of steaming and sun-drying cycle periods. It also discovered the anti-prostate cancer pathway through vector space network pharmacology, which gave researchers a fresh perspective for exploring the anti-prostate cancer active components of black ginseng and the mechanism of the effect of traditional Chinese medicine in the process of drug processing.

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High performance liquid chromatography-based method to analyze activity of GABA transporters in central nervous system

Cited by 2 publications

References 34 publications

Anti-prostate cancer mechanism of black ginseng during the "nine steaming and nine sun-drying" process based on HPLC analysis combined with vector space network pharmacology

Anti-prostate cancer mechanism of black ginseng during the "nine steaming and nine sun-drying" process based on HPLC analysis combined with vector space network pharmacology

Anti-prostate cancer mechanism of black ginseng during the "Nine Steaming and Nine Sun-Drying" process based on HPLC analysis combined with vector space network pharmacology

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