High-performance liquid chromatography assay for moxifloxacin: Pharmacokinetics in human plasma

Ulu, Sevgi Tatar

doi:10.1016/j.jpba.2006.06.026

Cited by 37 publications

(12 citation statements)

References 12 publications

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“…After these chemical reactions the sample was injected into the chromatographic system and SPA was determined through its derivative. Another fluorimetric HPLC method based on pre-column derivatization was developed for the quantification of MOX in plasma by Tatar Ulu [95]. In this method, MOX is derivatized with 4-chloro-7-nitrobenzodioxazole (NBD-CI) reagent and then determined by HPLC.…”

Section: Detectionmentioning

confidence: 99%

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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Fluoroquinolones are one of the most promising and intensively studied drugs of contemporary anti-infective chemotherapy. New fluoroquinolone antibacterials with improved pharmacokinetic properties and a broad spectrum of activity have been developed, opening new windows of opportunity for clinical use. To our knowledge, no comprehensive and critical review of the analytical methods for the determination of these agents, which correspond to the third- and fourth-generation quinolones, has yet been published. This work summarizes for the first time most of the liquid chromatographic methods reported in the literature for the separation and quantification of the new fluoroquinolones in biological matrices and pharmaceutical formulations. A systematic and detailed survey of physicochemical properties, sample preparation procedures, and chromatographic and detection conditions is presented herein. In the course of this review several liquid chromatographic methods are discussed: reversed-phase high-performance liquid chromatography (RP-HPLC), ion-exchange high-performance liquid chromatography (IEX-HPLC), hydrophilic interaction liquid chromatography (HILIC), high-performance thin-layer chromatography (HPTLC) and other chiral chromatographic methods. Their advantages, applicability and limitations are also examined.

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Section: Detectionmentioning

confidence: 99%

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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“…Spectrophotometric,4,5 spectrofluorimetric,6 liquid chromatographic,7–10 TLC,11 HPLC,12–16 Capillary electrophoresis,17,18 polarographic,19 and voltammetric20,21 procedures were applied for its determination.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Spectrophotometric, Atomic Absorption and Kinetic Methods for Determination of Moxifloxacin Hydrochloride

Abdellaziz

Hosny

2011

Anal Chem�Insights

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Three simple spectrophotometric and atomic absorption spectrometric methods are developed and validated for the determination of moxifloxacin HCl in pure form and in pharmaceutical formulations. Method (A) is a kinetic method based on the oxidation of moxifloxacin HCl by Fe3+ ion in the presence of 1,10 o-phenanthroline (o-phen). Method (B) describes spectrophotometric procedures for determination of moxifloxacin HCl based on its ability to reduce Fe (III) to Fe (II), which was rapidly converted to the corresponding stable coloured complex after reacting with 2,2′ bipyridyl (bipy). The formation of the tris-complex formed in both methods (A) and (B) were carefully studied and their absorbance were measured at 510 and 520 nm respectively. Method (C) is based on the formation of ion- pair associated between the drug and bismuth (III) tetraiodide in acidic medium to form orange—red ion-pair associates. This associate can be quantitatively determined by three different procedures. The formed precipitate is either filtered off, dissolved in acetone and quantified spectrophotometrically at 462 nm (Procedure 1), or decomposed by hydrochloric acid, and the bismuth content is determined by direct atomic absorption spectrometric (Procedure 2). Also the residual unreacted metal complex in the filtrate is determined through its metal content using indirect atomic absorption spectrometric technique (procedure 3). All the proposed methods were validated according to the International Conference on Harmonization (ICH) guidelines, the three proposed methods permit the determination of moxifloxacin HCl in the range of (0.8–6, 0.8–4) for methods A and B, (16–96, 16–96 and 16–72) for procedures 1–3 in method C. The limits of detection and quantitation were calculated, the precision of the methods were satisfactory; the values of relative standard deviations did not exceed 2%. The proposed methods were successfully applied to determine the drug in its pharmaceutical formulations without interference from the common excipients. The results obtained by the proposed methods were comparable with those obtained by the reference method.

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“…[23] Linearity Aliquots of 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, and 2.0 mL of the stock solution of moxifloxacin hydrochloride and ketorolac tromethamine were transferred into a series of 10 mL volumetric flasks and diluted to the mark with water to yield 2, 4, 6,8,10,12,14,16,18, and 20 μg/mL each of moxifloxacin hydrochloride and ketorolac tromethamine. The calibration curve was constructed by plotting peak areas versus concentrations, and the regression equation was calculated.…”

Section: Methods Validationmentioning

confidence: 99%

“…Prostaglandins have been shown to be mediators of certain kinds of intraocular inflammation and also produce disruption of the blood-aqueous humour barrier, vasodilation, increased vascular permeability, leukocytosis, and increased intraocular pressure. [5,6] A detailed survey of analytical literature for moxifloxacin hydrochloride revealed several methods based on varied techniques, viz, HPLC, [7][8][9][10] Spectrophotometry, [11,12] High-Performance Thin-Layer Chromatography (HPTLC). [13,14] Similarly, a survey of the analytical literature for ketorolac tromethamine revealed several methods like HPLC, [15,16] HPTLC, [17,18] LC/MS/MS (Liquid Chromatography-Mass Spectrometry-Tandem Mass Spectrometry), [19] capillary electro chromatography.…”

Section: Introductionmentioning

confidence: 99%

A validated stability-indicating high performance liquid chromatographic method for moxifloxacin hydrochloride and ketorolac tromethamine eye drops and its application in pH dependent degradation kinetics

Dave¹,

Vyas²,

Patel³

2013

Chron Young Sci

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Background and Aim:A fixed dose combination of moxifloxacin hydrochloride and ketorolac tromethamine is used in ratio of 1:1 as eye drops for the treatment of the reduction of post operative inflammatory conditions of the eye. A simple, precise, and accurate High Performance Liquid Chromatographic (HPLC) method was developed and validated for determination of moxifloxacin hydrochloride and ketorolac tromethamine in eye drops. Materials and Methods: Isocratic HPLC separation was achieved on a ACE C 18 column (C 18 (5 μm, 150 mm × 4.6 mm, i.d.)) using the mobile phase 10 mM potassium di-hydrogen phosphate buffer pH 4.6-Acetonitrile (75:25 v/v) at a flow rate of 1.0 mL/min. The detection was performed at 307 nm. Drugs were subjected to acid, alkali and neutral hydrolysis, oxidation and photo degradation. Moreover, the proposed HPLC method was utilized to investigate the pH dependent degradation kinetics of moxifloxacin hydrochloride and ketorolac tromethamine in buffer solutions at different pH values like 2.0, 6.8 and 9.0. Results and Conclusion: The retention time (t R ) of moxifloxacin hydrochloride and ketorolac tromethamine were 3.81±0.01 and 8.82±0.02 min, respectively. The method was linear in the concentration range of 2-20 μg/mL each for moxifloxacin hydrochloride and ketorolac tromethamine with a correlation coefficient of 0.9996 and 0.9999, respectively. The method was validated for linearity, precision, accuracy, robustness, specificity, limit of detection and limit of quantitation. The drugs could be effectively separated from different degradation products and hence the method can be used for stability analysis. Different kinetics parameters like apparent first-order rate constant, half-life and t 90 (time for 90% potency left) were calculated.

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High-performance liquid chromatography assay for moxifloxacin: Pharmacokinetics in human plasma

Cited by 37 publications

References 12 publications

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

Development and Validation of Spectrophotometric, Atomic Absorption and Kinetic Methods for Determination of Moxifloxacin Hydrochloride

A validated stability-indicating high performance liquid chromatographic method for moxifloxacin hydrochloride and ketorolac tromethamine eye drops and its application in pH dependent degradation kinetics

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