High Performance Liquid Chromatography and Ultra‐High Performance Liquid Chromatography Including Liquid Chromatography–Mass Spectrometry

Turfus, Sophie C.; Rodda, Luke N

doi:10.1002/9781119373421.ch14

Cited by 1 publication

(1 citation statement)

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“…LC-MS Analysis: LC-MS was performed on 15 HCC tissues and 15 adjacent nontumor tissues from 15 HCC patients, as previously described. [29] In an EP tube containing ≈100 mg sample, 1 mL tissue extract, and 3 steel beads were added. The samples were then placed in a tissue grinder and ground at 55 Hz for 2 min, followed by 30 min of ultrasound.…”

Section: Methodsmentioning

confidence: 99%

Effects of 3‐HAA on HCC by Regulating the Heterogeneous Macrophages—A scRNA‐Seq Analysis

Xue

Zheng

et al. 2023

Advanced Science

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Kynurenine derivative 3-hydroxyanthranilic acid (3-HAA) is known to regulate the immune system and exhibit anti-inflammatory activity by inhibiting T-cell cytokine secretion and influencing macrophage activity. However, the definite role of 3-HAA in the immunomodulation of hepatocellular carcinoma (HCC) is largely unexplored. An orthotopic HCC model and treated with 3-HAA by intraperitoneal injection is developed. Furthermore, cytometry by time-of-flight (CyTOF) and single-cell RNA sequencing (scRNA-seq) analyses are carried out to define the immune landscape of HCC. It is found that 3-HAA treatment can significantly suppress tumor growth in the HCC model and alter the level of various cytokines in plasma. CyTOF data shows that 3-HAA significantly increases the percentage of F4/80 hi CX3CR1 lo Ki67 lo MHCII hi macrophages and decreases the percentage of F4/80 lo CD64 + PD-L1 lo macrophages. scRNA-seq analyses demonstrate that 3-HAA treatment is proved to regulate the function of M1 macrophages, M2 macrophages, and proliferating macrophages. Notably, 3-HAA inhibits the proinflammatory factors TNF and IL-6 in multiple cell subsets, including resident macrophages, proliferating macrophages, and pDCs. This study reveals the landscape of immune cell subsets in HCC in response to 3-HAA, indicating that 3-HAA may be a promising therapeutic target for HCC.

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Section: Methodsmentioning

confidence: 99%