High performance liquid chromatographic quantification of salvinorin a from tissues ofsalvia divinorum epling & játiva-m

Determination of salvinorin A and salvinorin B in Salvia divinorum-related products circulated in Japan

“…The SalA/SalB contents (mg/mg) in the three dried leaf products were 3.2-5.0/0.10-0.17 mg/mg. The reported contents in dried leaf samples were 7.6-7.8 mg/mg [4] and 0.89-3.70 mg/mg [16] for SalA, as well as 4.2-10.4 mg/mg for SalB [4]. The SalA contents in our samples were roughly in accordance with these reported values.…”

“…The same authors analyzed methanol extract of the dried leaf by GC-MS without derivatization [15]. Gruber et al [16] and Wolowich et al [17] analyzed the respective chloroform extracts of the lyophilized leaf and the ''concentrated extract'' product by high-performance liquid chromatography (HPLC) with UV detection. Medana et al extracted the leaf by acetonitrilewater (1:1, v/v), and the extract was analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) [4].…”

“…The extraction procedures shown in the aforementioned References ( [14][15][16][17]4]) were not fully examined. For example, selection of extraction solvent, extraction efficiency, and how to remove pigments were not examined.…”

Neuropharmacology of the Naturally Occurring κ-Opioid Hallucinogen Salvinorin A

“…The presence of salvinorin A in botanical samples has been evaluated quantitatively by several methods. An early study described the use of high-performance liquid chromatography (HPLC) for determining the composition of salvinorin A in plant materials using UV detection (Gruber et al, 1999). This group demonstrated that the concentration of salvinorin A is highly variable between samples, showing composition ranging from 0.089 to 0.37%, with an average of 0.245%.…”

“…One study (Chavkin et al, 2004) reported the identification of [Mϩ23] precursor-related ions using electrospray ionization-high-resolution MS, whereas a later report identified two major product ions (m/z 295 and 373, [MϪ60] and [MϪ138], respectively) produced by collision-induced dissociation (Barnes et al, 2006). Schmidt et al (2005b) presented a method that demonstrated sensitivity 3 orders of magnitude greater than previously reported HPLC methods for the identification of salvinorin A in plants (Gruber et al, 1999).…”