High-performance liquid chromatographic method for the determination of gabapentin in human plasma

Zhu, Zhigang; Neirinck, Len

doi:10.1016/s1570-0232(02)00399-9

Cited by 57 publications

(44 citation statements)

References 13 publications

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“…Derivatization by chromophoric reagents increases the sensitivity of gabapentin detection. Several HPLC methods for determination of gabapentin in human plasma have been published using different derivatizing reagents such as 2,4,6-trinitrobenzenesulphonic acid, 3,4) phenylisothiocyanate, 5) o-phthaldialdehyde (OPA), [6][7][8][9][10][11][12] 4-chloro-7-nitrobenzofurazan, 13) 9-fluorenylmethyl chloroformate 14) and 1,2-naphthoquinone-4-sulphonic acid sodium salt. 15) Most of the proposed methods could be optimized for determination of gabapentin in dosage forms but they suffer from some limitations such as a lengthy run time or using special reaction conditions which may not be suitable for routine analysis.…”

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confidence: 99%

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Optimization of an HPLC Method for Determination of Gabapentin in Dosage Forms through Derivatization with 1-Fluoro-2,4-dinitrobenzene

Souri

Jalalizadeh

Shafiee

2007

CHEMICAL & PHARMACEUTICAL BULLETIN

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Section: )mentioning

confidence: 99%

“…Derivatization with phenylisothiocyanate (PITC) is simple, but this reagent degrades in contact with water and the reaction medium should be completely free from water before the addition of reagent. 5) Using 4-chloro-7-nitrobenzofurazan as a labeling reagent, the adduct should be extracted and detected by spectrofluorimetry.…”

Section: )mentioning

confidence: 99%

Optimization of an HPLC Method for Determination of Gabapentin in Dosage Forms through Derivatization with 1-Fluoro-2,4-dinitrobenzene

Souri

Jalalizadeh

Shafiee

2007

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Previous methods reported for the detection of GPT in biological fluids involved the use of liquid chromatography (LC) with ultraviolet (29)(30)(31) or fluorescence (32-37) detection or gas chromatography (GC) (38,39) and capillary electrophoresis (40). However, each of these published methods lacks a defensible method to further confirm the presence or absence of GPT in a test sample.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Gabapentin in Equine Plasma with Measurement Uncertainty Estimation by Liquid Chromatography-Tandem Mass Spectrometry

Liu

Uboh

Soma

et al. 2011

Journal of Analytical Toxicology

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Gabapentin (GPT) is an antiepileptic drug that was approved in 1993 for use in the management of neurotrophic pain and as an adjunctive therapy for refractory partial seizure in humans.It is also being tested in veterinary medicine as an adjunctive medication in the treatment of pain due to laminitis, neuropathic, or chronic pain. Gabapentin is readily available by prescription and even on the internet; therefore, it has the potential of being used in racehorses to mask pain. It is for this reason that a sensitive liquid chromatography-tandem mass spectrometry method has now been developed for the analysis of GPT in equine plasma and for studying the pharmacokinetic and pharmacodynamic profiles of GPT in the horse. Sample preparation was by rapid protein precipitation with acetonitrile. Analyte separation was achieved on a reversed-phase ACE C 18 column and analyzed by a hybrid triple-quadrupole linear ion trap mass spectrometer in positive electrospray ionization mode. Limits of detection, quantification, and confirmation of GPT were 1, 10, and 20 ng/mL, respectively. Calibration curve showed excellent linearity within the 10-2500 ng/mL range (r 2 > 0.999). Intra-and interday precision defined by coefficient of variation was <10%. Intra-and interday accuracy (bias %) was within 90-110%. Measurement uncertainty estimation was 8.6%. The method has been successfully used in the analysis of GPT in equine plasma following its administration to research horses for pharmacokinetic studies and in routine forensic analysis for doping control in racehorses in the State of Pennsylvania.

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“…Until now, several methods have been reported for quantification of GB in biological fluids. These methods were based on highperformance liquid chromatography (HPLC) coupled ultraviolet (UV) (Lensmeyer et al, 1995;Zhu and Neirinck, 2002) or fluorescence detection (Jiang and Li, 1999;Gauthier and Gupta, 2002); gas chromatography (GC) with flame ionization (Hooper et al, 1990;Wolf et al, 1996); or mass spectrometry (MS) (Borrey et al, 2005). However, most of these methods need to complicated sample preparation such as derivatization, protein precipitation or solid-phase extraction, and are thus laborious and time-comsuming.…”

Section: Introductionmentioning

confidence: 99%

Validated LC‐MS/MS method for quantification of gabapentin in human plasma: application to pharmacokinetic and bioequivalence studies in Korean volunteers

Park

Jhee

Park

et al. 2007

Biomedical Chromatography

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A sensitive validated liquid chromatography-tandem mass spectrometric method (LC-MS/MS) for gabapentin (GB) in human plasma has been developed and applied to pharmacokinetic (PK) and bioequivalence (BE) studies in human. In a randomized crossover design with a 1-week period, each subject received a 300 mg GB capsule. The procedure involves a simple protein precipitation with acetonitrile and separated by LC with a Gemini C(18) column using acetonitrile-10 mm ammonium acetate (20:80, v/v, pH 3.2) as mobile phase. The GB and internal standard [(S)-(+)-alpha-aminocyclohexanepropionic acid hydrate] were analyzed using an LC-API 2000 MS/MS in multiple reaction monitoring mode. The ionization was optimized using ESI(+) and selectivity was achieved using MS/MS analysis, m/z 172.0 --> 154.0 and m/z 172.0 --> 126.0 for GB and IS, respectively. The assay exhibited good linearity over a working range of 20-5000 ng/mL for GB in human plasma with a lower limit of quantitation of 20 ng/mL. No endogenous compounds were found to interfere with the analysis. The accuracy and precision were shown for concentrations over the standard ranges. This method was successfully applied for the PK and BE studies by analysis of blood samples taken up to 36 h after an oral dose of 300 mg of GB in 24 healthy volunteers.

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High-performance liquid chromatographic method for the determination of gabapentin in human plasma

Cited by 57 publications

References 13 publications

Optimization of an HPLC Method for Determination of Gabapentin in Dosage Forms through Derivatization with 1-Fluoro-2,4-dinitrobenzene

Optimization of an HPLC Method for Determination of Gabapentin in Dosage Forms through Derivatization with 1-Fluoro-2,4-dinitrobenzene

Analysis of Gabapentin in Equine Plasma with Measurement Uncertainty Estimation by Liquid Chromatography-Tandem Mass Spectrometry

Validated LC‐MS/MS method for quantification of gabapentin in human plasma: application to pharmacokinetic and bioequivalence studies in Korean volunteers

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