High-performance liquid chromatographic method for rapid and highly sensitive determination of histidine using postcolumn fluorescence detection with o-phthaldialdehyde

Tateda, Naohiro; Matsuhisa, Kiichi; Hasebe, Kiyoshi; Kitajima, Nagomi; Miura, Toshiaki

doi:10.1016/s0378-4347(98)00373-9

Cited by 47 publications

(30 citation statements)

References 38 publications

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“…19 As a result, separation of UCA isomers and histidine was achieved at 40˚C on a C18 reversed phase column with a 7:93 (v/v) mixture of acetonitrile and 20 mM phosphate buffer (pH 3.7) containing 5 mM sodium heptanesulfonate at a flow rate of 0.5 ml min -1 . After the separation, UV (264 nm) detector and the postcolumn derivatization with OPA were used for the sequential detections of UCA isomers and histidine.…”

Section: Hplc Methods For the Simultaneous Determinations Of Uca Isomementioning

confidence: 99%

“…After the separation, UV (264 nm) detector and the postcolumn derivatization with OPA were used for the sequential detections of UCA isomers and histidine. The postcolumn derivatization was carried out under the same conditions as our original ones 19 except that acetonitrile was used instead of methanol as a solvent for OPA. Figure 1 shows chromatograms of a standard mixture of 20 amino acids, cis-and trans-UCA, glutathione and pyroglutamic acid, the last one is known to present in the skin.…”

Section: Hplc Methods For the Simultaneous Determinations Of Uca Isomementioning

confidence: 99%

“…These improvements are probably due to precise temperature control (40˚C) of the eluent by insertion of a preheater coil in the line, as described in Experimental, such a coil is inherently required for the reproducible postcolumn detection of histidine with OPA. 19 …”

Section: Hplc Methods For the Simultaneous Determinations Of Uca Isomementioning

confidence: 99%

“…On the other hand, we have developed a specific HPLC method for the assay of serum histidine, 19 which is based on the separation by a reversed-phase ion pair chromatography followed by the selective detection of histidine with postcolumn fluorescence derivatization using o-phthalaldehyde (OPA).…”

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Simultaneous Determination of Urocanic Acid Isomers and Histidine in Human Stratum Corneum by High-Performance Liquid Chromatography

et al. 2001

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Section: Hplc Methods For the Simultaneous Determinations Of Uca Isomementioning

confidence: 99%

Section: Hplc Methods For the Simultaneous Determinations Of Uca Isomementioning

confidence: 99%

Section: Hplc Methods For the Simultaneous Determinations Of Uca Isomementioning

confidence: 99%

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Simultaneous Determination of Urocanic Acid Isomers and Histidine in Human Stratum Corneum by High-Performance Liquid Chromatography

et al. 2001

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“…Up to date, there have emerged lots of L-histidine detection methods, including capillary electrophoresis (Zhang and Sun, 2004;Zhou et al, 2010), liquid chromatography (Tateda et al, 1998), electrochemistry Huang et al, 2012;Liang et al, 2011), colorimetry Zhang et al, 2012b), spectrofluorimetry (Elbaz et al, 2008;Folmer-Andersen et al, 2005;Fu et al, 2013;He et al, 2013He et al, , 2012Huang et al, 2012;Kong et al, 2011;Li et al, 2013;Oliveira et al, 2013;Wu and Yan, 2010;Zhou et al, 2014). These methods, while most successful, some of them still suffer drawbacks, including cumbersome optical equipment, expensive robotic facilities and complicated operation procedures, tedious pre-treatment of the biological sample, or lower limit detection etc.…”

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Cu2+ modulated silver nanoclusters as an on–off–on fluorescence probe for the selective detection of l-histidine

Zheng

Yao

Zhu

et al. 2015

Biosensors and Bioelectronics

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A Highly Selective Luminescent Switch‐On Probe for Histidine/Histidine‐Rich Proteins and Its Application in Protein Staining

Wong

Chung

et al. 2008

Angewandte Chemie

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The luminescence sensing of histidine and histidine-rich proteins plays a pivotal role in biochemistry and molecular biology, in particular when both temporal and spatial resolution are required. An abnormal level of histidine-rich proteins is an indicator for many diseases, such as advanced liver cirrhosis, [1a,b] AIDS, [1c] renal disease, [1c] asthma, [1c] pulmonary disorders, [1d] thrombotic disorders, [1e, f] and malaria.[1g]Some analyses of histidine and histidine-rich proteins have been developed in conjunction with immunoassay [2a-d] and colorimetric detection methods.[2e] The most commonly used method for the detection of histidine and histidine-rich proteins in biological samples is chromatography, which is usually performed through the combination of an effective separation technique, such as thin-layer chromatography, gas chromatography, or HPLC, followed by UV/Vis or fluorescence spectroscopy.[2f-h] The use of high-performance capillary electrophoresis with a derivation reagent has also been reported.[2i] However, the aforementioned methods are generally tedious, laborious, and, most importantly, expensive for routine detection in a biochemistry laboratory.Although numerous studies have dealt with the detection of histidine or histidine-rich proteins, studies on the use of luminescent probes for this purpose remain sparse.[3] Notable examples include research by Fabbrizzi and co-workers, who developed competitive noncovalent fluorescence turn-on probes for histidine in the form of dizinc(II) or dicopper(II) macrocyclic complexes, which recognize histidine through the formation of an imidazolate bridge between the two dizinc(II) or dicopper(II) centers; [3a] however, the resulting noncovalent ensemble may be less stable than a covalently linking sensory system, and the complexity of the synthetic process makes it difficult to implement in a convenient manner.Photoluminescent iridium(III) complexes have emerged as a topical area of interest in inorganic photochemistry [4] and phosphorescent materials for optoelectronic [5] and luminescence signaling applications.[6] Significant changes in the photophysical behavior and emission properties of iridium-(III) complexes may be induced by the presence of biomolecules. Luminescent transition-metal complexes for protein staining, such as the luminescent ruthenium complex known as SYPRO Ruby dye, have been reported previously. [7] However, despite its high sensitivity and its broad dynamic range, the use of SYPRO Ruby dye is limited, as it is sold only as a formulated solution; therefore, it is not possible to optimize the dye for a particular electrophoresis protocol and protein. + , and the iridium complex is readily soluble and stable in aqueous staining solutions. In this study, [Ir(ppy) 3 ] (2) was also prepared for comparative studies, as its binding with proteins was expected to be largely hydrophobic in nature. Herein, we describe the luminescent switch-on probe [Ir(ppy) 2 (solv) 2 ] + (1) for histidine/histidinerich proteins and demonstrate...

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High-performance liquid chromatographic method for rapid and highly sensitive determination of histidine using postcolumn fluorescence detection with o-phthaldialdehyde

Cited by 47 publications

References 38 publications

Simultaneous Determination of Urocanic Acid Isomers and Histidine in Human Stratum Corneum by High-Performance Liquid Chromatography

Simultaneous Determination of Urocanic Acid Isomers and Histidine in Human Stratum Corneum by High-Performance Liquid Chromatography

Cu2+ modulated silver nanoclusters as an on–off–on fluorescence probe for the selective detection of l-histidine

A Highly Selective Luminescent Switch‐On Probe for Histidine/Histidine‐Rich Proteins and Its Application in Protein Staining

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