High-performance liquid chromatographic—mass spectrometric assay of high-value compounds for pharmaceutical use from plant cell tissue culture: Cinchona alkaloids

Sources and possible mechanisms of action of important phytoconstituents with cardiovascular properties

“…ledgeriana is also known as quinine tree and belongs to family Rubiaceae. The alkaloids found in the plant material, different transformed roots and tissue culture cells are quinine and quinidine (Abdulrahman et al, 1991). For centuries, the plant has been used as an antimalarial agent remained under observation for its antiarrythmic properties as converting atrial flutter and fibrillation to sinus rhythm, combating supraventricular and ventricular arrythmias, increasing the excitability threshold, prolonging effective refractory period.…”

Liquid chromatography coupled with mass spectrometry in the analysis of alkaloids

“…Under thermospray 'discharge-on' mode conditions, all alkaloids showed intense protonated molecules and hardly any fragmentation, except for the cinchophyllines. Abdulrahman et al (1991) used acetonitrile : acetic acid : 10 mM ammonium acetate (pH 3.0) (25 : 4 : 71) as eluent for the separation of the quinoline alkaloids from Cinchona plant materials and cell cultures. Strong protonated molecules were observed without fragmentation.…”

An Optimised Method for Routine Separation and Quantification of Major Alkaloids in Cortex Cinchona by HPLC Coupled with UV and Fluorescence Detection

“…Early TLC and GC methods were reviewed by Baerheim Svendsen and Verpoorte (, ). However, the most commonly used method has been HPLC using reversed phase chromatography (Abdulrahman et al , ; Gatti et al , ; Hobson‐Frohock and Edwards, ; Hoffmann et al , ; McCalley , , ; Smith, ; Theodoridis et al , ). Furthermore, a few capillary electrophoresis (CE) methods have also been published (Buchberger et al , ; Tsimachidis et al , ; Zhao et al , ).…”