High-Performance Liquid Chromatographic (HPLC) Separation and Quantitation of Endogenous Glucocorticoids after Solid-Phase Extraction from Plasma

Dawson, Ralph; Kontur, Paul J.; Monjan, Andrew

doi:10.1159/000179979

Cited by 58 publications

(16 citation statements)

References 9 publications

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“…One hundred l of the supernatant were stored at Ϫ20ЊC for later corticosterone (CORT) determination and 100 l at Ϫ80ЊC for catecholamine [noradrenaline (NA) and adrenaline (A)] measurements. For plasma CORT assessments, a reversedphase, high-performance liquid chromatography (HPLC) technique was used as described in Dawson, Kontur, & Monjan (1984). NA and A were extracted from the samples by using an extraction system as described in Smedes, Kraak, & Poppe (1982).…”

Section: Assessment Of Hormonal Levelsmentioning

confidence: 99%

Play is indispensable for an adequate development of coping with social challenges in the rat

et al. 1999

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Section: Assessment Of Hormonal Levelsmentioning

confidence: 99%

Play is indispensable for an adequate development of coping with social challenges in the rat

et al. 1999

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“…Plasma corticosterone was determined by reversed-phase high-performance liquid chromatography (Dawson, Kontur, & Monjan, 1984).…”

Section: Determination Of Hpa Axis Reactivitymentioning

confidence: 99%

Individual Behavioral Characteristics of Wild-Type Rats Predict Susceptibility to Experimental Autoimmune Encephalomyelitis

Kavelaars

Heijnen

Tennekes

et al. 1999

Brain, Behavior, and Immunity

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Individual behavioral characteristics of wild-type rats predict susceptibility to experimental autoimmune encephalomyelitis Kavelaars, A; Heijnen, CJ; Tennekes, R; Bruggink, Jan; Koolhaas, JM Neuroendocrine-immune interactions are thought to be important in determining susceptibility to autoimmune disease. Animal studies have revealed that differences in susceptibility to experimental autoimmune encephalomyelitis (EAE) are related to reactivity in the hypothalamo-pituitary-adrenal axis. It is known that there is a close relation between neuroendocrine parameters and behavioral characteristics, suggesting that behavior and disease susceptibility may be associated. In the present study we investigated whether behavioral characteristics of wild-type rats are related to susceptibility to disease. We show here that the latency of the animal to attack an intruder correlates significantly with the EAE disease score: animals that do not attack the intruder during the test period are more resistant to the disease than animals with short attack latency times. These data, obtained in an unselected strain of wild-type rats, demonstrate that behavioral response patterns of individual animals can in part predict susceptibility to autoimmune disease.

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“…Plasma levels of insulin, leptin and ACTH were measured by commercial radioimmunoassay kits (Linco Research; RI-13K, RL-83K and ACTH RIA kit, Nichols Institute Diagnostics, San Juan Capistrano, CA, USA). Plasma levels of corticosterone were measured using HPLC with UV detection according to Dawson et al, [22].…”

Section: Methodsmentioning

confidence: 99%

Brain Melanocortin Receptors are Involved In CRH-Mediated HPA Axis Activity And Thermogenesis

et al. 2011

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Recent studies indicate that corticotropin-releasing hormone (CRH) acts down-stream from brain melanocortin receptors (MC-Rs), however, much older evidence actually suggested the opposite. To reconcile this, rats were chronically infused centrally with the MC-R antagonist SHU9119 (0.5 nmol/day) over 14 days. Acute central CRH administration (0.5 μg) caused temporal anorexia, which was not impaired by SHU9119 treatment relative to controls. SHU9119 treatment, however, did attenuate CRH-induced plasma ACTH and thermogenic responses. Since SHU9119 pair-fed rats showed generally similar effects as SHU9119-treated ad libitum feeding rats, the modulation of ACTH and thermogenic responses are not secondary to increased body weight by SHU9119 treatment. Central pre-treatment with the glucocorticoid receptor agonist dexamethasone (10 μg) before CRH demonstrated that the inhibitory effect of SHU9119 treatment on the CRH-induced ACTH response was not attributed to increased glucocorticoid feedback. Peripheral dexamethasone pre-treatment (25 μg/kg rat), on the other hand, more potently suppressed CRH-induced corticosterone responses in SHU9119-treated rats. This effect is explained by sensitization of the adrenal cortex to glucocorticoid feedback as a result of SHU9119 treatment. We conclude that MC-Rs act down-stream from CRH to facilitate CRH-induced HPA axis activation and thermogenesis, but not down-stream from CRH to facilitate anorexia.

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High-Performance Liquid Chromatographic (HPLC) Separation and Quantitation of Endogenous Glucocorticoids after Solid-Phase Extraction from Plasma

Cited by 58 publications

References 9 publications

Play is indispensable for an adequate development of coping with social challenges in the rat

Play is indispensable for an adequate development of coping with social challenges in the rat

Individual Behavioral Characteristics of Wild-Type Rats Predict Susceptibility to Experimental Autoimmune Encephalomyelitis

Brain Melanocortin Receptors are Involved In CRH-Mediated HPA Axis Activity And Thermogenesis

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