High-performance liquid chromatographic determination of purine and pyrimidine bases, ribonucleosides, deoxyribonucleosides and cyclic ribonucleotides in biological fluids

Abreu, Ronney A. De; Baal, John M. van; Bruyn, C. H. M. M. de; Bakkeren, An A.J.M.; Schretlen, E. D. A. M.

doi:10.1016/s0378-4347(00)86037-5

Cited by 49 publications

(12 citation statements)

References 21 publications

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“…The blocker solution contained the adenosine deaminase inhibitor erythro-9(2-hydroxy-3-nonyl)-adenosine (10 uM), the adenosine transport inhibitor dipyridamole (20 OM) and the thrombocyte aggregation inhibitor indomethacine (2 mg/liter). Immediately after blood collection the blood/blocker mixture was centrifuged (model 3200; Eppendorf North America, Inc., Madison, WI) at 3,000 rpm for 2 min and the plasma was deproteinated with perchloric acid as described before (26). The extract was kept frozen at -20'C until the adenosine concentration was determined in duplicate by reversed-phase, high performance liquid chromatography using a nonlinear gradient.…”

Section: Methodsmentioning

confidence: 99%

Hemodynamic and neurohumoral effects of various grades of selective adenosine transport inhibition in humans. Implications for its future role in cardioprotection.

Rongen¹,

Smits²,

Donck³

et al. 1995

J. Clin. Invest.

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In 12 healthy male volunteers (27-53 yr), a placebo-controlled randomized double blind cross-over trial was performed to study the effect of the intravenous injection of 0.25, 0.5, 1, 2, 4, and 6 mg draflazine (a selective nucleoside transport inhibitor) on hemodynamic and neurohumoral parameters and ex vivo nucleoside transport inhibition. We hypothesized that an intravenous draflazine dosage without effect on hemodynamic and neurohumoral parameters would still be able to augment the forearm vasodilator response to intraarterially infused adenosine. Heart rate (electrocardiography), systolic blood pressure (Dinamap 1846 SX; Critikon, Portanje Electronica BV, Utrecht, The Netherlands) plasma norepinephrine and epinephrine increased dose-dependently and could almost totally be abolished by caffeine pretreatment indicating the involvement of adenosine receptors. Draflazine did not affect forearm blood flow (venous occlusion plethysmography). Intravenous injection of 0.5 mg draflazine did not affect any of the measured hemodynamic parameters but still induced a significant ex vivo nucleoside-transport inhibition of 31.5±4.1% (P < 0.05 vs placebo). In a subgroup of 10 subjects the brachial artery was cannulated to infuse adenosine (0.15, 0.5, 1.5, 5, 15, and 50 ,ug/100 ml forearm per min) before and after intravenous injection of 0.5 mg draflazine. Forearm blood flow amounted 1.9+0.3 ml/100 ml forearm per min for placebo and 1.8±0.2, 2.0±0.3, 3.8±0.9, 6.3±1.2, 11.3±2.2, and 19.3±3.9 ml/100 ml forearm per min for the six incremental adenosine dosages, respectively. After the intravenous draflazine infusion, these values were 1.6±0.2 ml/100 ml forearm per min for placebo and 2.1±0.3, 3.3±0.6, 5.8±1.1, 6.9±1.4, 14.4±2.9, and 23.5±4.0 ml/100 ml forearm per min, respectively (Friedman ANOVA: P < 0.05 before vs after draflazine infusion). In conclusion, a 30-50% inhibition of adenosine transport significantly augments the fore- arm vasodilator response to adenosine without significant systemic effects. These results suggest that draflazine is a feasible tool to potentiate adenosine-mediated cardioprotection in man. (J. Clin. Invest. 1995. 95:658-668.)

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Section: Methodsmentioning

confidence: 99%

Hemodynamic and neurohumoral effects of various grades of selective adenosine transport inhibition in humans. Implications for its future role in cardioprotection.

Rongen¹,

Smits²,

Donck³

et al. 1995

J. Clin. Invest.

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“…Purines and pyrimidines were determined by high-performance liquid chromatography according to a previously published method [7] and compared with normal values of purines and pyrimidines in CSF described before [8] and with control children for plasma. Heparinized plasma was centrifuged immediately after venipunc ture.…”

Section: Methodsmentioning

confidence: 99%

Disturbances of Cerebral Purine and Pyrimidine Metabolism in Young Children with Chronic Renal Failure

Gerrits

Monnens²,

Abreu³

et al. 1991

Nephron

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Chronic renal failure during childhood may be associated with delayed cognitive development. From 10 children with chronic renal failure, aged 2–59 months, plasma and cerebrospinal fluid (CSF) purines and pyrimidines have been determined. A marked increase of pseudouridine and cytidine was demonstrated in CSF of 10 and 8 children, respectively. The plasma concentration of pseudouridine was increased in a varying degree to a maximal value of more than 10 times the upper limit of normal. The plasma concentration of cytidine showed only moderately elevated values. In 3 children the study of CSF and plasma was repeated 6 weeks after the start of continuous ambulatory peritoneal dialysis. The abnormal concentrations of pseudouridine and cytidine were still present in CSF and plasma. Further studies are necessary to elucidate the cause of this unknown biochemical aberration of the central nervous system.

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“…The re coveries of (methyl)thiopurine nucleosides and bases from plasma with PCA are good. The recovery of 6MP from plasma with trichloro acetic acid is even better (94%) [9].…”

Section: Resultsmentioning

confidence: 99%

“…Previously, v/e published HPLC methods for the detection of purine and pyrimidine nu cleotides [8], nucleosides and bases [9] and thiopurine nucleosides and bases [10,11], In the present article we describe two HPLC methods, one for measurement of methylthiopurine and thiopurine nucleosides and bases, and another for measurement of (methyl)thiopurine nu cleotides. Both methods are used in the clinical randomized ALL-8 trial of the Dutch Childhood Leukaemia Study Group.…”

Section: W Ken' En K Amp Jansen Ct At I J Ch Rom a Togr B 672mentioning

confidence: 99%

Determination of extracellular and intracellular thiopurines and methylthiopurines by high-performance liquid chromatography

Keuzenkamp-Jansen¹,

Abreu²,

Bökkerink³

et al. 1995

Journal of Chromatography B: Biomedical Sciences and Applicatio

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The thiopurine antimetabolites 6-thioguanine and 6-mercaptopurine are important chemotherapeutic drugs in the treatment of childhood acute lymphoblastic leukaemia. Measurement of metabolites of these thiopurines is important because correlations exist between levels of these metabolites and the prognosis in childhood acute lymphoblastic leukaemia. The reversed-phase method for the determination of extracellular thiopurine nucleosides and bases was previously developed and has been modified such that methylthiopurine nucleosides, bases, thioxanthine and thiouric acid can be measured also. The anion-exchange method enables the determination of intracellular mono-, di-and triphosphate (methyl)thiopurine nucleotides in one run. Extraction on ice with perchloric acid and dipotassium hydrogenphosphate results in good recoveries for (methyl)thiopurine nucleotides in lymphoblasts and peripheral mononuclear cells and for methylthioinosine nucleotides in red blood cells. Measure ment of the low concentrations of mono-, di-and triphosphate thioguanine nucleotides in red blood cells (detection limit 20 pmol/109 cells) is possible after extraction with methanol and methylene chloride, followed by oxidation of thioguanine nucleotides with permanganate and fluorimetric detection.

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High-performance liquid chromatographic determination of purine and pyrimidine bases, ribonucleosides, deoxyribonucleosides and cyclic ribonucleotides in biological fluids

Cited by 49 publications

References 21 publications

Hemodynamic and neurohumoral effects of various grades of selective adenosine transport inhibition in humans. Implications for its future role in cardioprotection.

Hemodynamic and neurohumoral effects of various grades of selective adenosine transport inhibition in humans. Implications for its future role in cardioprotection.

Disturbances of Cerebral Purine and Pyrimidine Metabolism in Young Children with Chronic Renal Failure

Determination of extracellular and intracellular thiopurines and methylthiopurines by high-performance liquid chromatography

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