High-performance liquid chromatographic determination of morphine and its 3- and 6-glucuronide metabolites by two-step solid-phase extraction

Meng, Qing Cheng; Cepeda, M. Soleded; Krämer, T.; Zou, Helen; Matoka, Derek J.; Farrar, John T.

doi:10.1016/s0378-4347(00)00146-8

Cited by 54 publications

(22 citation statements)

References 40 publications

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“…HPLC with UV detection is a common analytical method for these drugs in biological samples. However, many reports describe the need for multiple extractions and complex chromatographic systems to ensure reproducibility and resolution [19][20][21][22][23][24][25]. Recently, a method employing HPLC combined with electro spray ionization-tandem mass spectrometry (HPLC-ESI-MS-MS) for low level quantitation of MOR, M3G and M6G has been described [26].…”

Section: Discussionmentioning

confidence: 99%

Relationship between plasma concentrations of morphine and its metabolites and pain in cancer patients

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Relationship between plasma concentrations of morphine and its metabolites and pain in cancer patients

et al. 2010

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“…Morphine concentrations in plasma and brain were determined by HPLC with electrochemical detector as previously described. 15) Plasma sample (200 ml) was mixed with 50 ml of 2 mM naloxone (internal standard) and 800 ml of 0.5 M ammonium sulfate (pH 9.3). Brain sample was homogenized in 4 volumes of saline.…”

Section: Methodsmentioning

confidence: 99%

Antinociceptive Effects of St. John's Wort, Harpagophytum Procumbens Extract and Grape Seed Proanthocyanidins Extract in Mice

Uchida

Hirai

Hatanaka³

et al. 2008

Biological & Pharmaceutical Bulletin

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Currently, the consumption of dietary supplement containing botanical products and foods is growing at a remarkable speed, in terms of the promotion of health or prevention and treatment of diseases. The extract from Hypericum perforatum (St. John's wort, SJW) possess clinical efficacy in the therapy of mild to moderate depression. 1,2) The most important constituents of SJW are phloroglucinols such as hyperforin and pseudohyperforin, naphthodianthrones such as hypericin and pseudohypericin, in addition to flavonoids such as rutin, quercetin, quercitrin. Several in vitro studies have indicated that SJW and hyperforin may act via a blockade of reuptake of serotonin, noradrenaline and dopamine in similar manner as most of the current antidepressants such as tricyclic antidepressants, [3][4][5] which have been known to exhibit antinociceptive properties by monoamine reuptake blockade.Harpagophytum procumbens extract (HPE) and Grape seed proanthocyanidins extract (GSPE) have been reported to exert anti-inflammatory activity in rodents.6-8) Harpagophytum procumbens commonly known as Devil's claw is an herbaceous plant, growing specifically in Southern Africa. Preparations of its secondary roots contain iridoid glycosides, mainly harpagoside, harpagogide and procumbide. HPE have been shown to possess clinical efficacy in the treatment of degenerative rheumatoid arthritis, osteoarthritis and tendonitis. [6][7][8] In addition, experimental study has revealed antiinflammatory activity of HPE in Freund's adjuvant-induced arthritis model. 9) Proanthocyanidins are naturally occurring polyphenolic compounds widely available in fruits, vegetables, nuts, seeds, flowers and bark. Grape seed proanthocyanidins, a combination of biologically active polyphenolic flavonoids including oligomeric proanthocyanidins, have been shown to exert a novel spectrum of biological, pharmacological, therapeutic and chemoprotective properties against oxygen free radicals and oxidative stress. GSPE protects against free radicals models and has exhibited superior antioxidant performance as compared to vitamin C, E and b-carotene. 10)Previous studies with antidepressant activity of SJW and anti-inflammatory effects of HPE and GSPE have led to the idea that these herbal products exert antinociceptive action. Thus, the aim of this study was to clarify the antinociceptive properties of SJW, HPE and GSPE after oral administration to mice with mechanisms that might potentially underlie these activities. The effects of these herbal extracts on the antinociception and plasma and brain concentrations of morphine were also examined. MATERIALS AND METHODSDrugs SJW and HPE were kindly donated by Indena (Milan, Italy). SJW was standardized to the content of hypericin (0.3%) and hyperforin (3.2%) and HPE was also standardized to the content of harpagoside (1.9%). GSPE was kindly supplied by Kikkoman Co. (Chiba, Japan), and standardized to the content of proanthocyanidin (83.9%). Morphine hydrochloride was purchased from Takeda Pharmaceutical Co. (Osaka, Japan). A...

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“…So far, several methods of analytical techniques have been reported to determine MO concentrations such as highperformance liquid chromatography (HPLC) [7,8], liquid chromatography with UV detection [9,10], GC-mass spectroscopy [11][12][13][14], LC-mass spectrometry detection [15], and electrochemical methods [16][17][18][19][20]. However, some of these techniques are time consuming, contain interferences of matrix, use large biological fluid volumes, and need expensive instruments and toxic solvents, and in some cases, special pretreatment is required before analysis.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of electrochemical sensor based on molecularly imprinted polymer and nanoparticles for determination trace amounts of morphine

Rezaei

Foroughi-Dehnavi

Ensafi

2015

Ionics

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A simple and rapid electrochemical method developed for the detection of trace amounts of morphine (MO) at the surface of modified pencil graphite electrode (PGE) with multiwall carbon nanotubes (MWCNTs), molecularly imprinted polymer (MIP), and gold nanoparticles (AuNPs).Various types of electrochemical methods containing cyclic voltammetry (CV), square wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS) were employed to probe the characteristics of the constructed electrode toward morphine. After optimization of several effective parameters, the calibration curve by SWV was linear in two linear domains, over the range of 0.008 to 5 μmol L −1 vs. Ag/AgCl, and the detection limit was 2.9 nM. The relative standard deviation (RSD) for six replicate determinations of 0.05 μmol L −1 MO was found to be 2.57 % (S/N = 3). Finally, the ability of the electrochemical sensor was successfully applied for MO determination in real samples such as human urine and plasma.

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High-performance liquid chromatographic determination of morphine and its 3- and 6-glucuronide metabolites by two-step solid-phase extraction

Cited by 54 publications

References 40 publications

Relationship between plasma concentrations of morphine and its metabolites and pain in cancer patients

Relationship between plasma concentrations of morphine and its metabolites and pain in cancer patients

Antinociceptive Effects of St. John's Wort, Harpagophytum Procumbens Extract and Grape Seed Proanthocyanidins Extract in Mice

Fabrication of electrochemical sensor based on molecularly imprinted polymer and nanoparticles for determination trace amounts of morphine

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