High-performance liquid chromatographic determination of tranilast in plasma

Utility of a novel turn-off fluorescence probe for the determination of tranilast, an adjunctive drug for patients with severe COVID-19

“…The literature survey represented limited methods for the determination of TR, including spectrophotometry 22 and highperformance liquid chromatography (HPLC). [23][24][25] These methods suffer from many limitations, such as low sensitivity, [22][23][24][25] long chromatographic run times, [23][24][25] complicated sample preparation 25 and using non-green solvents. [23][24][25] For example: the reported methods 23,25 use high methanol percentage up to 60% and the reported method 24 use high percentage of acetonitrile which is considered a toxic and non-green solvent.…”

“…[23][24][25] These methods suffer from many limitations, such as low sensitivity, [22][23][24][25] long chromatographic run times, [23][24][25] complicated sample preparation 25 and using non-green solvents. [23][24][25] For example: the reported methods 23,25 use high methanol percentage up to 60% and the reported method 24 use high percentage of acetonitrile which is considered a toxic and non-green solvent. Our study aimed to examine AC quenching for the quick spectrouorimetric determination of TR depending on the carboxylic group in TR that could interact with AC at an appropriate pH.…”

Liquid chromatographic/tandem mass spectrometric method for the quantitation of tranilast in human plasma

“…Separation by reversed-phase highperformance liquid chromatography using methanol/10 mM ammonium acetate (70: 30, v/v) A number of assays have been developed for the determination of tranilast in biological samples using highperformance liquid chromatography (HPLC) with ultraviolet (UV) detection. [4][5][6] These methods suffer from a number of limitations including low sensitivity, long chromatographic run times, 4-6 and complicated sample preparation. …”