High-performance liquid chromatographic determination of glycoalkaloids in potatoes from conventional, integrated, and organic crop systems

Abreu, Pedro; Relva, Angela M.; Matthew, Susan; Gomes, Zulmira; Morais, Zilda

doi:10.1016/j.foodcont.2005.08.005

Cited by 25 publications

(12 citation statements)

References 19 publications

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“…Decreased levels of glycoalkaloids in organically grown potatoes agrees with the findings of Hellenas and Branzell (1995) and Abreu et al (2007), although Hajšlová et al (2005) reported slightly higher levels of glycoalkaloids in organically grown tubers but with year-to-year and genotypic variation swamping any effects of management practice.…”

Section: Lc-mssupporting

confidence: 86%

Effect of agricultural production systems on the potato metabolome

et al. 2013

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Section: Lc-mssupporting

confidence: 86%

Effect of agricultural production systems on the potato metabolome

et al. 2013

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“…There is no information available on carry-over of tropane alkaloids from feed into animal derived products under normal livestock production conditions. The (Mateus et al 2000), thin layer-chromatography (TLC) (Simonovska and Vovk 2000), gas chromatography-mass spectrometry (GC-MS) (Laurila et al 1999;Miraldi et al 2001;Balikova 2002), high-pressure liquid chromatography (HPLC) (Bucher et al 1989;Papadoyannis et al 1993;Cieri 2005;Kodamatani et al 2005;Abreu et al 2007;Jensen et al 2007), liquid chromatography-mass spectrometry (LC-MS) (Matsuda et al 2004;Breton et al 2005;Siluk et al 2007;Distl et al 2009), liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Xu et al 1995;Chen et al 2006).…”

Section: Figurementioning

confidence: 99%

“…Reported extraction procedures for tropane alkaloids in plants and biological fluids usually involve liquid-liquid extraction (LLE) (Xu et al 1995;Miraldi et al 2001) with non-miscible solvents or solid-phase extraction (SPE) (Balikova 2002;Chen et al 2006). Most of the reported solvents used for GAs extraction are based on weak acidic solutions with the addition of other solvents or salts (Eltayeb et al 2003/4;Matsuda et al 2004;Kodamatani et al 2005;Abreu et al 2007;Machado et al 2007).…”

Section: Chromatographic Separationmentioning

confidence: 99%

Development of a liquid chromatography-tandem mass spectrometric method for the simultaneous determination of tropane alkaloids and glycoalkaloids in crops

Jandrić

Rathor

Švarc‐Gajić

et al. 2011

Food Additives & Contaminants: Part A

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International audienceA new, rapid and sensitive multiresidue method is reported for the simultaneous determination of tropane alkaloids (tropine, atropine, scopolamine, homatropine, anisodamine) and glycoalkaloids (α-solanine, α-chaconine) in grains and seeds (wheat, rye, maize, soybean, linseed). Dispersive solid phase extraction (DSPE) was performed with 0.5% formic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. For a fast and effective clean-up procedure for oily matrices such as soybean and linseed, matrix solid phase dispersion (MSPD) C18 material was used to remove co-extracted non-polar components. No clean-up was necessary for less oily matrices following extraction. The analytes were separated by isocratic HPLC on a Chirobiotic V column and detected using a triple quadrupole mass spectrometer with electrospray ionization (ESI). All analytes were monitored in the positive ion mode. The method performance is presented in terms of linearity in the range 5-80 ng/g (r2=0.998), specifity, selectivity, accuracy (recoveries from 61-111%), precision (CV < 5%) and ruggedness. The limits of quantitation (LOQ) were in the range 2.2-4.9 ng/g

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“…However, factors associated with growth, harvest and postharvest treatments, such as drought (Bejarano et al 2000), high temperature (Morris and Petermann 1985;Lafta and Lorenzen 2000), light exposure of tubers (Dale et al 1993;Percival et al 1994;Grunenfelder et al 2006) and wounding (Bergenstråhle et al 1992;Choi et al 1994), may elevate tuber SGA content. Moreover, organically grown potatoes have been reported to contain elevated levels of glycoalkaloids (Hajslova et al 2005;Wszelaki et al 2005;Abreu et al 2007). Presumably environmental factors uniquely associated with organic management induce the biosynthesis of natural resistant factors, including SGAs (Friedman 2006).…”

Section: Introductionmentioning

confidence: 99%

Potato Steroidal Glycoalkaloids: Biosynthesis and Genetic Manipulation

2008

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The potato steroidal glycoalkaloids (SGAs) are important components of plant resistance against pests and pathogens but can be toxic to humans at high levels. SGAs derive their toxicity from anticholinesterase activity affecting the central nervous system and the disruptive effects on cell membrane integrity affecting the digestive system and other organs. Accordingly, current safety regulations limit their content in the edible tuber to 20 mg per 100 g fresh weight. SGA composition and level are genetically determined, with unfavourable growth conditions and inappropriate postharvest management inducing the accumulation of SGAs at levels in the tubers of "safe" cultivars beyond the maximum level set by the industry. Hence, genetic alteration of potato to prevent toxic levels of SGAs in tubers is highly desirable. At the same time, maintaining high SGA levels in other plant organs will contribute to plant resistance against pathogen and pest attacks. To this end, SGA biosynthesis and degradation should be manipulated precisely to exploit tissue-specific expression rather than whole-plant suppression of SGA production, to produce potato cultivars with SGA content enriched in the foliage but diminished in the edible tubers. Only a few details are known about the SGA biosynthetic pathway, its genes and intermediates. Research on factors that regulate SGA biosynthesis and catabolism as well as searches for genetic markers linked to total and specific SGA levels have only recently been pursued. The present review summarizes current data on these issues to encourage further discussion on SGA manipulation for safer food products.

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High-performance liquid chromatographic determination of glycoalkaloids in potatoes from conventional, integrated, and organic crop systems

Cited by 25 publications

References 19 publications

Effect of agricultural production systems on the potato metabolome

Effect of agricultural production systems on the potato metabolome

Development of a liquid chromatography-tandem mass spectrometric method for the simultaneous determination of tropane alkaloids and glycoalkaloids in crops

Potato Steroidal Glycoalkaloids: Biosynthesis and Genetic Manipulation

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