High-performance liquid chromatographic determination of hypotaurine and taurine after conversion to 4-dimethylaminoazobenzene-4′-sulfonyl derivatives and its application to the urine of cysteine-administered rats

Effect of glucose-cysteine adduct as a cysteine prodrug in rats

“…Total sulfate (free plus ester) was determined by ion chromatography after hydrolysis as described . Taurine, and hypotaurine if any, was determined by reversed phase high-performance liquid chromatography after conversion to 4-dimethylaminoazobenzene-4'-sulfonyl-taurine (dabsyltaurine), and dabsyl-hypotaurine if any (Futani et al, 1994).…”

Separation methods for taurine analysis in biological samples

“…The bulk of these are relatively involving pre-or interfere with the determination of taurine, purificapost-column derivatization followed by ultraviolet, tion procedures by a cation-exchange column fluorescence and / or electrochemical detection to 1 (Dowex 50W, 2.0 ml, 5.530.7 cm I.D., H form) improve both the quality of separation as well as [35] or an ion-exchange column filled with both an detection sensitivity. An obvious advantage of preanionic and cationic resin (Amberlite AG138, 200-column derivatization relatively to post-column de-400 mesh, 0.5 ml and Amberlite AG50W-38, 200-rivatization is that it requires a basic HPLC system 400 mesh, 1 ml) have been proved practical.…”

“…Futani et al conductivity detection. It cannot be detected with [35] found that dabsylhypotaurine once formed was UV detection due to the lack of an ultraviolet very stable, and it was not converted into chromophore. Zhu and co-workers [59,60] showed dabsyltaurine on storage at room temperature for that taurine, leucine and phenylalanine could be several months or on flash evaporation at 40 8C.…”

“…[23] for the pre-column derivatization of taurine for the treated aqueous sample solution containing taurine high sensitivity, reliability and perfect reproducibility and hypotaurine with freshly powdered 3,5-dinitro- [2,35]. Stocchi et al [2] presented a gradient HPLC benzoyl chloride in the presence of triethylamine.…”

“…Two tography with suppressed conductivity detection. methods have been reported to successfully solve Since the dissociation constant pK (4.96) of taurine a this problem [23,35]. One was by using DABS-Cl as is too large, it cannot be detected sensitively with the pre-column derivatizing reagent.…”