High-performance liquid chromatographic determination of the insulin sensitizing agent DRF-2189 in rat plasma

Oral Bioavailability and Pharmacokinetics of the New lnsulin Sensitizer DRF-2189 in Wistar Rats

“…Plasma sample preparation and assay was the same as described elsewhere [10]. To 0.1 ml of plasma, the internal standard (troglitazone, 3 µg) was added and DRF-2189 was extracted using 2.0 ml of ethylacetate:dichloromethane (3:2; v/v) as extraction solvent.…”

“…The HPLC system consisted of a Waters LC Module-l (Waters, Milford, MA, USA), Perkin Elmer LC-240 fluorescence detector (Perkin Elmer, Buckinghamshire, England, UK), Millennium software (Waters, Milford, MA, USA) and a HiChrom C 18 (ODS) column (5 µm, 4.6 mm x 250 mm, HiChrom Limited, Berks, UK). Analysis of DRF-2189 was carried out using a validated HPLC method with fluorescence detection described elsewhere [10]. The mobile phase used was 0.05 mol/l sodium dihydrogen orthophosphate buffer: acetonitrile: methanol (22.5:37.5:40; v/v, pH 5.0) at a flow rate of 1.0 ml/min.…”

Determination of troglitazone stereoisomers in rat plasma using semi-micro HPLC with electrochemical detection

“…1. Some HPLC methods have been reported and validated for the analysis of troglitazone in pharmaceutical and biological samples [3][4][5][6], however, it would be impossible to detect each stereoisomer of troglitazone using these methods. In general, separation of stereoisomers has become very important in analytical chemistry, especially in the pharmaceutical and biological fields, because some stereoisomers of racemic drugs have quite different pharmacokinetic properties and different pharmacological or toxicological effects [7][8][9].…”