High-performance liquid chromatographic determination of tocopherols in infant formulas

Alkaline saponification results in decomposition of tocopherols in milk and ovine blood plasma

“…It is noteworthy that ␥-T and ␤-T have the same chemical formula, differing only in the methyl group substitution pattern, and have similar relative antioxidant activities [4]. In line with previous reports [1,2,11], ␣-T, ␦-T and ␥-T as well as ␣-TAc were used in the study, as the native concentration of ␤-T in foods is usually below the limits of UV or fluorescence detection or significantly lower compared with the concentration of ␣-T and other tocopherols.…”

“…Tocopherols, also known as vitamin E, consist of a group of isoprenoid compounds of plant origin, which were first described as essential micronutrients for normal fertility in rats [1][2][3]. Later studies have established a wide range of physiological functions for this vitamin, e.g.…”

Simultaneous analysis of carotenoids and tocopherols in botanical species using one step solid–liquid extraction followed by high performance liquid chromatography

“…For HPLC analysis, dry extracts were dissolved in 1.5 mL of n-hexane/2-propanol (99/1, v/v), and filtered through a 0.22 lm syringe filter (hydrophilic Durapore PVDF, Millipore, Darmstadt, Germany). All the redissolved extracts were kept at À20°C in amber-coloured vials, and analysed before a week to ensure reproducible results (Irakli, Samanidou, & Papadoyannis, 2011;Rodrigo, Alegría, Barberá, & Farré, 2002).…”

Stability of fatty acids and tocopherols during cold storage of human milk

“…Standard solutions of a-T (284 mg L À1 ), b-T (50 mg mL À1 ), g-T (62.5 mg L À1 ) and d-T (263 mg L À1 ) were prepared in n-hexane. The rest of reagents and HPLC system conditions were as previously reported by Rodrigo, Alegría, Barberá, and Farré (2002).…”

“…Tocopherol extraction was carried out according to the method previously described by Rodrigo et al (2002), using 1 mL of sample (fresh, refrigerated or frozen).…”