High-performance liquid chromatographic determination of the antifungal drug fluconazole in plasma and saliva of human immunodeficiency virus-infected patients

Koks, C. H. W.; Rosing, Hilde; Meenhorst, Pieter L.; Bult, A.; Beijnen, Jos H.

doi:10.1016/0378-4347(94)00445-b

Cited by 58 publications

(44 citation statements)

References 13 publications

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“…This can be summarized as the following: The literature survey reveals several analytical methods used for quantitative determination of FLZ in body fluids and pharmaceutical preparations. These methods include high-performance liquid chromatography [2][3][4][5][6][7][8] , liquid chromatography-tandem mass spectrometry [9][10][11] , gas chromatography [12][13][14][15] ,micellar electrokinetic capillary chromatography 16 and bioassay 17 .…”

Section: Theory Of Ratio Difference Spectrophotometric Methods (Rdsm)mentioning

confidence: 99%

Novel Spectrophotometric Methods for the Determination of Fluconazole in the Presence of Its Oxidative Degradation Product

Lotfy

Monir

El-Aleem

2012

J. Chil. Chem. Soc.

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Five sensitive, selective and precise spectrophotometric stability-indicating methods for the determination of fluconazole in the presence of its oxidative degradation product and in pharmaceutical formulations are developed and validated. Method A is a newly developed spectrophotometric one that is called ratio difference method by measuring the difference in amplitudes between 261.5 and 266.5 nm of ratio spectrum. Method B is a third derivative spectrophotometric one, which determines peak amplitudes from 265.5-268nm (peak-peak). Method C is ratio subtraction method. Method D is the first derivative of the ratio spectra by measuring the peak amplitude at 269 nm. Finally, method E is the mean centering of ratio spectra method by measuring the peak amplitude at 266.5 nm. The proposed methods allow the determination of fluconazole in the presence of its oxidative degradation product over a concentration range of 50-400 µg.mL -1 with mean percentage recoveries of 100.24±0.91, 99.91±0.52, 100.20±1.26, 99.79±0.69, and 100.77±0.72 respectively.The selectivity of the proposed methods is checked using laboratory prepared mixtures. The proposed methods have been successfully applied to the analysis of fluconazole in pharmaceutical dosage forms without interference from other dosage form additives and the results have been statistically compared with a reported method.

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Section: Theory Of Ratio Difference Spectrophotometric Methods (Rdsm)mentioning

confidence: 99%

Novel Spectrophotometric Methods for the Determination of Fluconazole in the Presence of Its Oxidative Degradation Product

Lotfy

Monir

El-Aleem

2012

J. Chil. Chem. Soc.

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show abstract

“…Reportou-se ainda que tais procedimentos, pela presença do grande número de componentes endógenos, principalmente proteínas solúveis presentes no extrato, tem como conseqüência a troca freqüente das pré-colunas, redução da vida útil da coluna analítica, além de entupimento da tubulação interna e do injetor automático do sistema cromatográfico gerando aumento de pressão e ainda vazamentos que prejudicam a operacionalização (EGLE et al, 2004;COCIGLIO et al, 1996;INAGAKI et al, 1992;KOKS et al, 1994;NG et al, 1996;WALLACE et al, 1992;WATTANANAT & WIYADA, 2006). O maior problema desses métodos anteriormente reportados está relacionado a baixa seletividade e sensibilidade (COCIGLIO et al, 1996;INAGAKI et al, 1992;KOKS et al, 1994;WALLACE et al, 1992;WATTANANAT & WIYADA, 2006).…”

Section: Validação Do Método Para Determinação De Fluconazolunclassified

“…Quanto às condições cromatográficas, reportou-se para a maioria dos métodos anteriores que utilizaram coluna de fase reversa C18 e fase móvel binária contendo mistura tamponada sob fluxo de 1mL/min (EGLE et al, 2004;COCIGLIO et al, 1996;INAGAKI et al, 1992;KOKS et al, 1994;NG et al, 1996;WALLACE et al, 1992;WATTANANAT & WIYADA, 2006).…”

Section: Validação Do Método Para Determinação De Fluconazolunclassified

“…Em conformidade com trabalhos anteriores, optou-se pelo comprimento de onda de 210 nm que apresenta boa sensibilidade para o fluconazol (EGLE et al, 2004;COCIGLIO et al, 1996;INAGAKI et al, 1992;KOKS et al, 1994;NG et al, 1996;WALLACE et al, 1992;WATTANANAT & WIYADA, 2006). Por outro lado, uma série de interferentes são detectados já que muitas substâncias não-específicas mostram considerável absorbância nesse comprimento de onda.…”

Section: Validação Do Método Para Determinação De Fluconazolunclassified

“…Inúmeros métodos foram reportados para determinação de anfotericina B e fluconazol em matrizes biológicas através da cromatografia líquida de alta eficiência; entretanto, a maioria apresentou baixa seletividade, além de problemas relacionados a reprodutibilidade, linearidade e estabilidade dos fármacos (ALAK et al, 1996;COCIGLIO et al, 1996;EGGER et al, 2001;EGLE et al, 2004;ELDEM & ARICAN-CELLAT, 2001;INAGAKI et al, 1992;KIM & LIM, 1984;KOKS et al, 1994;LAMBROS et al, 1996;LOPEZ et al, 1998;LOPEZ-GALERA et al, 1995;NG et al, 1996;WALLACE et al, 1992;WATTANANAT & WIYADA, 2006). Sendo assim, objetivo do presente estudo foi desenvolver método bioanalítico robusto em cromatografia líquida de alta eficiência para o fluconazol e para a anfotericina B em matrizes biológicas que reunisse os requisitos de boa especificidade e seletividade, alta sensibilidade e larga linearidade, garantindo-se ainda precisão e exatidão aceitáveis além de baixo custo relativo.…”

Section: -Introduçãounclassified

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Monitoramento de antifúngicos em plasma e líquor de pacientes portadores de meningite criptocócica e AIDS através de cromatografia líquida de alta eficiência UV/Vis

Perez¹

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Application of capillary zone electrophoresis with off-line solid-phase extraction toin vitro metabolism studies of antifungals

Crego¹,

Gómez²,

Marina³

et al. 2001

Electrophoresis

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A simple and robust solid-phase extraction (SPE) procedure for the cleanup and sample preconcentration of antifungals (ketoconazole, clotrimazole, itraconazole, fluconazole, and voriconazole) and their metabolites after incubation with human liver microsomes, as well as a simplified capillary zone electrophoresis (CZE) method for their rapid analysis, have been developed to determine the stability of these compounds in in vitro samples. Three different sample pretreatment procedures using SPE with reversed-phase sorbents (100 mg C8, 100 mg C18, and 30 mg Oasis-HLB) were studied. The highest and most reproducible recoveries were obtained using a 30 mg Oasis-HLB sorbent and methanol containing 2% acetic acid as eluent. Enrichment by a factor of about four times was achieved by reconstituting the final SPE eluates to a small volume. For the CZE separation, good separations without interfering peaks due to the in vitro matrix were obtained with a simple running electrolyte using a fused-silica capillary. The best separation for all components originated by each tested drug after incubation with human liver microsomes (unmetabolized parent drug and its metabolites) was obtained using a 0.05 M phosphate running buffer (pH 2.2) without additives. The effect of the injection volume was also investigated in order to obtain the best sensitivity. Performance levels in terms of precision, linearity, limits of detection, and robustness were determined.

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High-performance liquid chromatographic determination of the antifungal drug fluconazole in plasma and saliva of human immunodeficiency virus-infected patients

Cited by 58 publications

References 13 publications

Novel Spectrophotometric Methods for the Determination of Fluconazole in the Presence of Its Oxidative Degradation Product

Novel Spectrophotometric Methods for the Determination of Fluconazole in the Presence of Its Oxidative Degradation Product

Monitoramento de antifúngicos em plasma e líquor de pacientes portadores de meningite criptocócica e AIDS através de cromatografia líquida de alta eficiência UV/Vis

Application of capillary zone electrophoresis with off-line solid-phase extraction toin vitro metabolism studies of antifungals

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