High-Performance Liquid Chromatographic Determination of Pilocarpine in Aqueous Humor: Derivatization by Quaternization of Methylimidazole Tertiary Amine Group

Mitra, Ashim K.; Baustian, C.L.; Mikkelson, Thomas J.

doi:10.1002/jps.2600690303

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Cited by 19 publications

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“…However, the resultant volume change was taken into consideration when calculating the amount of drug released. The concentration of pilocarpine in the solution was determined by using a Variane E UV at 217 nm (Varian Inc., Victoria, Australia) 13. All the release experiments were repeated at least three times, and the average value is reported herein.…”

Section: Methodsmentioning

confidence: 99%

Physical Characterization of a Chitosan‐Based Hydrogel Delivery System

2002

Journal of Pharmaceutical Sciences

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Section: Methodsmentioning

confidence: 99%

Physical Characterization of a Chitosan‐Based Hydrogel Delivery System

2002

Journal of Pharmaceutical Sciences

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General Aspects of Precolumn Derivatization with Emphasis on Pharmaceutical Analysis

Sternson¹

1981

Chemical Derivatization in Analytical Chemistry

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Decomposition of pilocarpine eye drops assessed by a highly efficient high pressure liquid chromatographic method

Kuks

Weekers

Goldhoorn

1990

Pharmaceutisch Weekblad Scientific Edition

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A rapid high-resolution high pressure liquid chromatographic method was developed for assaying pilocarpine. Pilocarpine in ophthalmic solutions decomposes fairly rapidly to give isopilocarpine, pilocarpic acid and isopilocarpic acid. The quality of an ophthalmic solution can be assessed by assaying these decomposition products. Existing high pressure liquid chromatographic methods suffer from long analysis times and poor resolution. The new method uses as the mobile phase 6 ml/l of triethylamine in water (pH 2.3, adjusted with 85% phosphoric acid) at a flow of 1.5 ml/min and as the stationary phase a C18-silica 125 x 4.6 mm column. 2-Amino-1-phenyl-1,3-propanediol is used as an internal standard. Complete separation was obtained within 8 min. Pilocarpine eye drops were stored under different conditions and then analysed for decomposition products. During heat treatment, decomposition to isopilocarpine predominated over decomposition to pilocarpic or isopilocarpic acid. However, when stored at room temperature or in a refrigerator, formation of pilocarpic acid clearly prevailed. Thus, from assessment of decomposition products, the cause of decomposition can be established.

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High-Performance Liquid Chromatographic Determination of Pilocarpine in Aqueous Humor: Derivatization by Quaternization of Methylimidazole Tertiary Amine Group

Cited by 19 publications

References 6 publications

Physical Characterization of a Chitosan‐Based Hydrogel Delivery System

Physical Characterization of a Chitosan‐Based Hydrogel Delivery System

General Aspects of Precolumn Derivatization with Emphasis on Pharmaceutical Analysis

Decomposition of pilocarpine eye drops assessed by a highly efficient high pressure liquid chromatographic method

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