High-performance liquid chromatographic determination of kanamycin

Amikacin Assay in Serum by High-Performance Liquid Chromatography

“…Amikacin was eluted with methanol-EDTA as the mobile phase at a flow rate of 2.0 ml/min and detected by determining relative fluorescence. The filters used in the fluorometer covered the extinction and emission wavelengths described for derivatives of o-phthalaldehyde (8). Quantitation was based on integration of peak areas with an integrating recorder previously calibrated with known concentations of amikacin.…”

Aminoglycoside antibiotics — two decades of their hplc bioanalysis

“…The general term kanamycin includes at least three known aminoglycosides, namely kanamycin A, kanamycin B and kanamycin C (Mays et al, 1976).…”

High-Performance Liquid Chromatography Techniques and Applications

“…Typical here are the aminoglycosides; exemplified by gentamicin. Here, pre-column derivatisation with dansyl chloride (1 27) or o-phthalaldehyde (128) or post-column methods (129,130) to yield fluorescent derivatives have been found useful. Many of the reported methods have also been developed to examine serum samples so that extraction processes are described and highly sensitive methodology is available.…”