“…Amikacin was eluted with methanol-EDTA as the mobile phase at a flow rate of 2.0 ml/min and detected by determining relative fluorescence. The filters used in the fluorometer covered the extinction and emission wavelengths described for derivatives of o-phthalaldehyde (8). Quantitation was based on integration of peak areas with an integrating recorder previously calibrated with known concentations of amikacin.…”
“…Amikacin was eluted with methanol-EDTA as the mobile phase at a flow rate of 2.0 ml/min and detected by determining relative fluorescence. The filters used in the fluorometer covered the extinction and emission wavelengths described for derivatives of o-phthalaldehyde (8). Quantitation was based on integration of peak areas with an integrating recorder previously calibrated with known concentations of amikacin.…”
“…Typical here are the aminoglycosides; exemplified by gentamicin. Here, pre-column derivatisation with dansyl chloride (1 27) or o-phthalaldehyde (128) or post-column methods (129,130) to yield fluorescent derivatives have been found useful. Many of the reported methods have also been developed to examine serum samples so that extraction processes are described and highly sensitive methodology is available.…”
The techniques and applications of high‐performance liquid chromatography, with particular reference to pharmaceutical and clinical sciences, are reviewed.
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