High-performance liquid chromatographic determination of electrically neutral carbohydrates with conductivity detection

Ligand-exchange indirect conductometric detection of electrically neutral carbohydrates and alcohols after high-performance liquid chromatographic separation

“…The results obtained by the previous experiments show that very slight amounts of polyols formed the borate complexes in the solutions (12). One can, therefore, make an approximation of Cp = [P] = [P]* and Cp' = [P'].…”

“…Carbohydrates and alcohols are required to be measured sensitively in many fields, because they are important both chemically and biologically. As a result of fundamental and applicable investigations, many workers have noticed that high-performance liquid chromatography (HPLC) is a useful tool for measuring these compounds and have published many papers dealing with HPLC separation and detection (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12).…”

“…In the previous paper (12), the author reported that electrically neutral sugars eluted from a cation exchange resin column are detectable with conductometry using a boric acid eluent and that this method is highly sensitive relative to the other detection methods such as refractive index detection.…”

Targeted borate complex formation as followed with electrospray ionization Fourier transform ion cyclotron mass spectrometry: monomolecular model system and polyborate formation

“…Borate complex formation with polyols has been analyzed by potentiometric or conductometric titrations26 or indirectly with high‐performance liquid chromatography (HPLC) coupled to conductivity detection. Here, the detection of electrically neutral carbohydrates was feasible only in the presence of boric acid as an eluent 27–29. Capillary electrophoresis methods have also been developed for separation of acidic monosaccharides and their corresponding 1,4‐lactones in a single run without further modification 30.…”

Development of fast pulsed electrochemical detection of carbohydrates separated by liquid chromatography and capillary electrophoresis

“…Acyl derivatives of carbohydrates using benzoyl chloride (23) and p-nitrobenzoyl chloride (24) also are used. Although carbohydrates are not detected directly by conductivity, their borate complexes are acidic enough to allow detection by conductometric detection (25).…”