High-performance liquid chromatographic determination of enalapril in human plasma by enzyme kinetic analytical method

LC–MS/MS method for the determination of several drugs used in combined cardiovascular therapy in human plasma

“…These LOQs were validated in terms of relative standard deviation (RSD) and RE < 20% and signals at least 10 times higher than the blank's response. The working ranges were defined considering the LOQ, the normal therapeutic dosage and the time needed to achieve the maximum plasmatic levels [7,[10][11][12][13][14][20][21][22][23][24].…”

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

“…[1][2][3] Different analytical methods have been applied to the determination of the remaining drugs analyzed by us, and also by HPLC techniques. Many of the HPLC analytical methods exist for the assay of ENA, [4][5][6][7] SOT, [8][9][10][11][12][13][14][15] CAP, [16][17][18][19][20][21][22][23][24] MET, [25][26][27][28][29][30][31] 62,63 and SPI [64][65][66][67][68] in different biological fluids and tissues. HPLC methods were applied to the simultaneous quantification of selected β-blockers (also: SOT, MET and PRO) in human fluids (plasma, serum, urine).…”

Determination of angiotensin converting enzyme inhibitory activity by high-performance liquid chromatography/electrospray-mass spectrometry

“…Compared to the previous method based on the substrate of hippuryl dipeptides HGG or HHL, the incubation time in the new method was approximately one-fourth of that of the conventional screening method using HPLC-UV or spectrophotometer. In the literature, the incubation time of HPLC or spectrophotometry method was about 35-60 min [9][10][11][12][13][14] for purified ACE, over 60 [35,36] min for human plasma ACE. The advantage of reduced incubation time mainly benefits from the much lower limit of quantification of ESI-MS than HPLC with ultraviolet detection.…”

“…In order to validate the newly developed method in this paper, IC 50 (the concentration of inhibitor required to inhibit 50% of the ACE activity) values of benazeprilat obtained by the four assays were compared with those obtained by conventional HPLC method [15,36]. To make sure that the results obtained by the conventional HPLC method were comparable with those by the proposed method, some modifications were made to the conventional HPLC method, i.e.…”