High-performance liquid chromatographic assay of hydroxyl free radical using salicylic acid hydroxylation during in vitro experiments involving thiols

Diez, Laurent; Livertoux, Marie-Hélène; Stark, Avishay‐Abraham; Wellman-Rousseau, Maria; Leroy, Pierre

doi:10.1016/s0378-4347(01)00396-6

Cited by 99 publications

(41 citation statements)

References 24 publications

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“…The abundance of MG can be indirectly determined by measuring the levels of 2-MQ (17). LC/MS analysis was conducted to determine whether ADTIQ accumulated in the brains of rats with diabetes.…”

Section: Measurement Of Mg and Adtiq Levels In The Brains Of Rats Witmentioning

confidence: 99%

Formation of a salsolinol-like compound, the neurotoxin, 1-acetyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, in a cellular model of hyperglycemia and a rat model of diabetes

Song

Xin

Xie

et al. 2013

International Journal of Molecular Medicine

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Abstract. There are statistical data indicating that diabetes is a risk factor for Parkinson's disease (PD). Methylglyoxal (MG), a biologically reactive byproduct of glucose metabolism, the levels of which have been shown to be increase in diabetes, reacts with dopamine to form 1-acetyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (ADTIQ); this formation may provide further insight into the connection between PD and diabetes. In this study, we investigated the role of ADTIQ in these two diseases to determine in an aim to enhance our understanding of the link between PD and diabetes. To this end, a cell model of hyperglycemia and a rat model of diabetes were established. In the cell model of hyperglycemia, compared with the control group, the elevated glucose levels promoted free hydroxyl radical formation (p<0.01). An ADTIQ assay was successfully developed and ADTIQ levels were detected and quantified. The levels of its precursors, MG and dopamine (DA), were determined in both the cell model of hyperglycemia and the rat model of diabetes. The proteins related to glucose metabolism were also assayed. Compared with the control group, ADTIQ and MG levels were significantly elevated not only in the cell model of hyperglycemia, but also in the brains of rats with diabetes (p<0.01). Seven key enzymes from the glycolytic pathway were found to be significantly more abundant in the brains of rats with diabetes. Moreover, it was found that adenosine triphosphate (ATP) synthase and superoxide dismutase (SOD) expression levels were markedly decreased in the rats with diabetes compared with the control group. Therefore, ADTIQ expression levels were found to be elevated under hyperglycemic conditions. The results reported herein demonstrate that ADTIQ, which is derived from MG, the levels of which areincreased in diabetes, may serve as a neurotoxin to dopaminergic neurons, eventually leading to PD. IntroductionDiabetes mellitus (DM), as a state of chronic hyperglycemia, and Parkinson's disease (PD) are diseases which consist a global health threat. In recent years, there have been increasing data indicating that hyperglycemia is associated with an increased risk of developing PD (1,2). However, the mechanisms underlying the association between hyperglycemia and PD have not yet been elucidated.Deng and Rajput (6) were the first to report, in 2001, that 1-acetyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (ADTIQ), a salsolinol-like compound, is found at highly concentrated levels in the brains of patients who suffer from PD (3). When the brains of deceased patients with PD were compared to those of normal subjects, it was found that patients with PD presented elevated levels of ADTIQ in all the examined brain areas. This finding indicated that elevated ADTIQ expression levels may be one of the mechanisms involved in the increased risk patients with diabetes have of developing PD (4).Formation of a salsolinol-like compound, the neurotoxin, 1-acetyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, in a cellular model of hyperglycem...

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Section: Measurement Of Mg and Adtiq Levels In The Brains Of Rats Witmentioning

confidence: 99%

Formation of a salsolinol-like compound, the neurotoxin, 1-acetyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, in a cellular model of hyperglycemia and a rat model of diabetes

Song

Xin

Xie

et al. 2013

International Journal of Molecular Medicine

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“…22,23,24,25 The gradient mobile phase flow was almost same with dexketoprofen analysis. The temperature was 25°C.…”

Section: Ultra Performance Liquid Chromatography Assay Methods and Valmentioning

confidence: 99%

Development and Validation of an Ultra Performance Liquid Chromatography Method for the Determination of Dexketoprofen Trometamol, Salicylic Acid and Diclofenac Sodium

Ilbasmiş-Tamer

2017

tjps

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ÖZAmaç: Deksketoprofen trometamol (DKP), salisilik asit (SA) ve diklofenak sodyumun (DIC) ilaç çözeltisindeki analizi için ultra yüksek basınçlı sıvı kromatografisi (UPLC) kullanılarak basit, hızlı, doğru ve kesin bir yöntem geliştirilmiştir. Gereç ve Yöntemler: UPLC yöntemi aktif bileşikleri analiz etmek için oldukça hassas bir yöntemdir ve yöntem ICH kurallarına göre valide edilmiştir. Bulgular: Geliştirilen yöntem, kesin, doğru, spesifik ve seçici bulunmuştur. Yöntem, doğrusal ve tekrarlanabilir bulunmuştur. DKP maddesi için teşhis sınırı-duyarlılık sınırı (LOD) 0.00325 µg/mL ve tayin alt sınırı-saptama sınırı (LOQ) 0.00985 µg/mL olarak bulunmuştur. SA için LOD 0.0027 µg/mL ve LOQ 0.0081 µg/mL olarak bulunmuştur. DIC için LOD 0.0304 µg/mL ve LOQ 0.0920 µg/mL olarak bulunmuştur. Sonuç: Böylece önerilen yöntemler yukarıda bahsedilen ilaçları içeren (deksketoprofen trometamol, salisilik asit ve diklofenak sodyum) farmasötik preparatlarda başarılı bir şekilde uygulanabilecektir. Aktif bileşikler çok küçük miktarlarda analiz edilebilecek ve kolaylıkla valide edilebilecektir. Anahtar kelimeler: Deksketoprofen trometamol, salisilik asit, diklofenak sodyum, UPLC, validasyon Objectives: A simple, fast, accurate and precise method has been developed for the determination of dexketoprofen trometamol (DKP), salicylic acid (SA) and diclofenac sodium (DIC) in the drug solutions using ultra high performance liquid chromatography (UPLC). Materials and Methods: UPLC method is highly reliable and sensitive method to quantify the amount of the active ingredient and the method is validated according to ICH guidelines. Results: The developed method is found to be precise, accurate, specific and selective. The method was also found to be linear and reproducible. The value of limit of dedection (LOD) of DKP, SA, DIC were found 0.00325 µg/mL, 0.0027 µg/mL and 0.0304 µg/mL, respectively. The limit of quantitation (LOQ) of DKP, SA and DIC were found 0.00985 µg/mL, 0.0081 µg/mL and 0.0920 µg/mL, respectively. Conclusion: Proposed methods can be successfully applicable to the pharmaceutical preparation containing the above mentioned drugs (dexketoprofen trometamol, salicylic acid and diclofenac sodium). Even very small amounts of active substance can be analyzed and validations can be performed easily.

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“…Commonly used methods for detecting · OH include electron spin resonance (ESR) [7][8][9] ; chemiluminescence 10,11) ; and high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, 12) electrochemical detection (ED), [13][14][15] and fluorometric detection. 16,17) The ESR method has been widely accepted to measure · OH scavenging capacity, but it requires expensive instrumentation and cannot be readily used to obtain quantitative estimates of · OH adducts because the · OH spin is unstable and may react with other products.…”

Section: Notesmentioning

confidence: 99%

“…Edaravone, L-tryptophan, gallic acid, Trolox, N-acetyl-L-cysteine, and ascorbic acid are commonly used as · OH scavenging samples. 10,13,21,22) Figure 5 shows a typical FIA profile obtained from Trolox. As shown in Fig.…”

Section: Effect Of Flow Rates and Mixing Coil Lengthmentioning

confidence: 99%

Selective Detection of Hydroxyl Radical Scavenging Capacity Based on Electrogenerated Chemiluminescence Detection Using Tris(2,2'-bipyridine)ruthenium(III) by Flow Injection Analysis

Nobushi

Uchikura

2010

CHEMICAL & PHARMACEUTICAL BULLETIN

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We describe a new method for detecting hydroxyl radical scavenging capacity based on tris(2,2-bipyridine)ruthenium(III) [Ru(bpy) 3 3؉] chemiluminescence and flow injection analysis. Hydroxyl radicals were generated by the Fenton reaction. The scavenging capacity of the six antioxidants tested decreased in the following order: edaravoneϾL-tryptophanϾgallic acidϾTroloxϾN-acetyl-L-cysteineϾascorbic acid. The proposed method allowed a sample throughput of about 80 samples/h. The six antioxidants were found to inhibit chemiluminescence intensity of Ru(bpy) 3 2؉. The proposed method is a rapid, selective, and accurate procedure for the study of hydroxyl radical scavenging capacity by Ru(bpy) 3 3؉ ؉ chemiluminescence.

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High-performance liquid chromatographic assay of hydroxyl free radical using salicylic acid hydroxylation during in vitro experiments involving thiols

Cited by 99 publications

References 24 publications

Formation of a salsolinol-like compound, the neurotoxin, 1-acetyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, in a cellular model of hyperglycemia and a rat model of diabetes

Formation of a salsolinol-like compound, the neurotoxin, 1-acetyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, in a cellular model of hyperglycemia and a rat model of diabetes

Development and Validation of an Ultra Performance Liquid Chromatography Method for the Determination of Dexketoprofen Trometamol, Salicylic Acid and Diclofenac Sodium

Selective Detection of Hydroxyl Radical Scavenging Capacity Based on Electrogenerated Chemiluminescence Detection Using Tris(2,2'-bipyridine)ruthenium(III) by Flow Injection Analysis

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