high-performance liquid chromatographic assay of vitamin k in human serum

Lefevere, M.F.; Leenheer, A.P. De; Claeys, A.

doi:10.1016/s0021-9673(00)95293-2

Cited by 48 publications

(7 citation statements)

References 16 publications

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“…Only cord blood samples provided enough material, but showed varying vita min Ki contents due to differences in analyt ical accuracies [7,29,31], including our ear lier results [27,29], using the method de scribed by Lefevere et al [22], Methods applying postcolumn reduction combined with fluorescence detection [22,25] seemed to cope with accuracy problems and with the limited amount of blood available from newborns. Applying the HPLC method described by Van Haard et al [10], we were able to measure vitamin Ki in small serum samples, and as we needed only 5 ml venous blood from neonates to investigate both vita min Ki and coagulation parameters, we could investigate each child several times in 4 weeks.…”

Section: Discussionmentioning

confidence: 76%

Vitamin K<sub>1</sub> Levels and Coagulation Factors in Healthy Term Newborns till 4 Weeks after Birth

Bruyn

Haard²,

Beunis³

et al. 1990

Pathophysiol Haemos Thromb

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Vitamin K₁ serum levels were assessed by means of an off-line multidimensional liquid chromatography in 18 mothers, shortly after delivery, and in their healthy term infants. Umbilical cord and venous blood samples were assayed up to 4 weeks of life. Concurrently, levels of coagulation factors II and X, antithrombin III and platelets were established. Although the detection limit of the assay was as low as 22 pg/ml, vitamin K₁ concentration appeared to be still beyond that level in cord blood or in newborn serum within 30 min after birth, whereas vitamin-K-dependent coagulation factors are already at a level of 40 %, without evidence for the presence of descarboxy prothrombin, in any of the investigated neonates. After 3 days, breast-fed neonates had lower vitamin K₁ levels than formula-fed infants (0.76 and 1.44 ng/ml, respectively). The levels of the vitamin-K-dependent coagulation factors II and X, however, were comparable, regardless of the kind of feeding. After 28 days, breast-fed neonates had even lower vitamin K₁ levels (0.49 ng/ml, while the formula-fed infants showed higher vitamin K₁ levels (4.45 ng/ml). But even then, the levels of vitamin-K-dependent coagulation factors II and X were comparable, regardless of the kind of feeding. From this we conclude that the serum levels of vitamin K₁ in formula-fed neonates exceed those of breast-fed infants from the moment of feeding (24 h and later) without a concomitant rise in vitamin-K-dependent coagulation factors. A relationship between vitamin K₁ levels and vitamin-K-dependent coagulation factors could not be established in healthy term breast-fed or formula-fed infants.

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Section: Discussionmentioning

confidence: 76%

Vitamin K<sub>1</sub> Levels and Coagulation Factors in Healthy Term Newborns till 4 Weeks after Birth

Bruyn

Haard²,

Beunis³

et al. 1990

Pathophysiol Haemos Thromb

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“…Detection modes include ultraviolet [11], electrochemical [12], fluorescence [13], chemiluminescence [14] and mass spectrometry [15].…”

Section: Introductionmentioning

confidence: 99%

Determination of vitamin K1 in plasma by solid phase extraction and HPLC with fluorescence detection

Paroni

Faioni

Razzari

et al. 2009

Journal of Chromatography B

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“…To determine normal levels of K1 in serum samples with a UV absorption detector, a sequential adsorption and reversed-phase HPLC procedure was developed (22). Obviously, this procedure lengthens the analysis time over a single chromatographic separation coupled to a more selective detector.…”

Section: Photoreduction/fluorescence Detection Of Quinones (Prfq)mentioning

confidence: 99%

“…Because vitamin K¡ (2-methyl-3-phytyl-l,4-naphthoquinone) is extremely hydrophobic, its determination after extraction from matrices that contain lipophilic interferents is quite challenging. To determine normal levels of Kx in serum samples with a UV absorption detector, a sequential adsorption and reversed-phase HPLC procedure was developed (22). Obviously, this procedure lengthens the analysis time over a single chromatographic separation coupled to a more selective detector.…”

Section: Introductionmentioning

confidence: 99%

Photoreduction fluorescence detection of quinones in high-performance liquid chromatography

Poulsen¹,

Birks²

1989

Anal. Chem.

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A slmple, reagent-free photochemlcai reactlon detector for quinones Including, but not ilmited to, the K vitamins is presented. Quinones are photoreduced to dihydroqulnones vla hydrogen abstraction from the hlgh-performance liquid chromatography (HPLC) moblie phase in a postcolumn photoreactor. An advantage over previous reaction-detection methods for quinones Is the slmpiiclty of thls system. No postcolumn reagent addttlon pumps are required. Additionally, the reaction Is accompilshed In a very short reactor which mlnhnlres band broadening and anatysis time. Detectlon Ihnits for many qukrone derlvatlves are In the low -ram range with good selectlvlty. I n this paper, the photochemistry of the detection scheme and its optimization for use in HPLC are discussed. As a sample appllcatlon, the detection of vitamin K, in a plant extract (Capsella bursa pasfw/us) without prefractlonatlon Is Included. A new photoreactor housing for carrying out anaeroblc reactions In low-dlsperslon, PTFE photoreactors is described.

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high-performance liquid chromatographic assay of vitamin k in human serum

Cited by 48 publications

References 16 publications

Vitamin K<sub>1</sub> Levels and Coagulation Factors in Healthy Term Newborns till 4 Weeks after Birth

Vitamin K<sub>1</sub> Levels and Coagulation Factors in Healthy Term Newborns till 4 Weeks after Birth

Determination of vitamin K1 in plasma by solid phase extraction and HPLC with fluorescence detection

Photoreduction fluorescence detection of quinones in high-performance liquid chromatography

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