High-performance liquid chromatographic assay for morphine in small plasma samples: Application to pharmacokinetic studies in rats

Domínguez-Ramírez, Adriana Miriam; Cortés-Arroyo, Alma Rosa; Peña, Marcela Hurtado y de la; Aoki-Maki, Kazuko; López, José Raúl Medina; Ríos-Castañeda, Camilo; López‐Muñoz, Francisco

doi:10.1016/j.jpba.2005.08.031

Cited by 11 publications

(6 citation statements)

References 36 publications

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“…A minimal remaining signal intensity of 85% was considered acceptable. Stability in plasma was not studied; the high stability of these analytes, during both long-term storage (−20 and −80°C) and processing (4-7 and 20-22°C) has been described extensively and the reader is referred to the literature regarding MDZ/OHM (Link et al, 2007;Li et al, 2007) and MOR/M3G/M6G (Taylor and Elliott, 2009;Dominguez-Ramirez et al, 2006).…”

Section: Validation Proceduresmentioning

confidence: 99%

Quantification of midazolam, morphine and metabolites in plasma using 96‐well solid‐phase extraction and ultra‐performance liquid chromatography–tandem mass spectrometry

Ahsman

Nagel

Mathôt

2010

Biomedical Chromatography

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Currently, pharmacokinetic-pharmacodynamic studies of sedatives and analgesics are performed in neonates and children to find suitable dose regimens. As a result, sensitive assays using only small volumes of blood are necessary to determine drug and metabolite concentrations. We developed an ultra-performance liquid chromatographic method with tandem mass spectrometry detection for quantification of midazolam, 1-hydroxymidazolam, hydroxymidazolamglucuronide, morphine, morphine-3-glucuronide and morphine-6-glucuronide in 100 microL of plasma. Cleanup consisted of 96 wells micro-solid phase extraction, before reversed-phase chromatographic separation (ultra-performance liquid chromatography) and selective detection using electrospray ionization tandem mass spectrometry. Separate solid-phase extraction methods were necessary to quantify morphine, midazolam and their metabolites because of each group's physicochemical properties. Standard curves were linear over a large dynamic range with adequate limits of quantitation. Intra- and interrun accuracy and precision were within 85-115% (of nominal concentration using a fresh calibration curve) and 15% (coefficient of variation, CV) respectively. Recoveries were >80% for all analytes, with interbatch CVs (as a measure of matrix effects) of less than 15% over six batches of plasma. Stability in plasma and extracts was sufficient, allowing large autosampler loads. Runtime was 3.00 min per sample for each method. The combination of 96-well micro-SPE and UPLC-MS/MS allows reliable quantification of morphine, midazolam and their major metabolites in 100 microL of plasma.

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Section: Validation Proceduresmentioning

confidence: 99%

Quantification of midazolam, morphine and metabolites in plasma using 96‐well solid‐phase extraction and ultra‐performance liquid chromatography–tandem mass spectrometry

Ahsman

Nagel

Mathôt

2010

Biomedical Chromatography

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“…Prior to analysis, spiked calibration standards and quality control samples were prepared in rat brain tissue homogenates for all the treatment compounds at appropriate concentrations that ranged from 1.0 to 2250 ng/mL. The LC‐MS/MS analyses of morphine, M3G and pregabalin were performed separately as previously described (Dominguez‐Ramirez et al., ; Oertel et al., ) with some modifications. The chromatographic separations were achieved on Atlantis HILIC Silica column (3‐μm particle size, 100 mm × 2.1 mm I.D., Waters, Milford, MA, USA) using a gradient elution of mobile phase consisting of acetonitrile and 10 mmol/L ammonium formate in 0.2% formic acid (v/v).…”

Section: Methodsmentioning

confidence: 99%

Pregabalin enhances the antinociceptive effect of oxycodone and morphine in thermal models of nociception in the rat without any pharmacokinetic interactions

Jokinen

Lilius

Laitila

et al. 2015

European Journal of Pain

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“…These include: spectrophotometry 5,6 , immuno chromatography 7 , potentiometry 8,9 , simultaneous voltammetric and amperometric 10 , gc-mass 1,11,26 , cyclicvoltametry and amperometry 12 , cyclicvoltametry 13,14 , sequential injection analysis 4 ,chemiluminescence 15 , kinetic potentiometric 9 , high performance liquid chromatography [16][17][18][19] , gas chromatography 20 , capillary electrophoresis 21 , ion mobility spectrometry 22,23 . These methods are efficient, but require expensive instrument and are expensive and many of them have needing time to complete the determination.…”

Section: Oriental Journal Of Chemistrymentioning

confidence: 99%

Kinetic Spectrophotometric Determination of Morphine in Pharmaceutical Samples

Keyvanfard¹,

Amri²

2014

Orient J Chem

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A new, sensitive, simple, inexpensive and fast kinetic spectrophotometric method was developed for the determination of trace amounts of morphine over the range of 12-60ng/mL. The method is based on the catalytic effect of morphine on the reaction of bromate and methylene bluein acidic media is reported. The reaction was monitored spectrophotometrically by 60 ng/ml measuring the decrease in absorbance of methylene blue at 665 nm with a fixed-time 0.5-2.5 min from initiation of the reaction. The detection limit is 0.8 ng/mL and relative standard deviation of 12and 52ng/mLmorphine for 6 replicate measurements was 1.50 and 0.87%respectively. The method 12 and 52 ng/ml was applied to the determination of morphine inpharmaceutical samples % respectively.

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High-performance liquid chromatographic assay for morphine in small plasma samples: Application to pharmacokinetic studies in rats

Cited by 11 publications

References 36 publications

Quantification of midazolam, morphine and metabolites in plasma using 96‐well solid‐phase extraction and ultra‐performance liquid chromatography–tandem mass spectrometry

Quantification of midazolam, morphine and metabolites in plasma using 96‐well solid‐phase extraction and ultra‐performance liquid chromatography–tandem mass spectrometry

Pregabalin enhances the antinociceptive effect of oxycodone and morphine in thermal models of nociception in the rat without any pharmacokinetic interactions

Kinetic Spectrophotometric Determination of Morphine in Pharmaceutical Samples

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