High-performance liquid chromatographic analysis of nitroxoline in plasma and urine

Sorel, R; Snelleman, C.; Hulshoff, A.

doi:10.1016/s0378-4347(00)81056-7

Cited by 12 publications

(18 citation statements)

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“…It has been reported that severely tailing peaks are obtained for nitroxoline with frequently used chromatographic systems reversed-phase, ion-exchange, and dynamic ion-exchange [2]. It has been suggested that strong interaction of nitroxoline with the free silanol groups of the column-packing material and the presence of trace metal ions were responsible for the tailing but addition of neither 8-hydroxyquinoline [2] nor Ni ion [3] to the mobile phase improved the separation of nitroxoline from structurally related compounds.…”

Section: Optimization Of the Hplc Separationmentioning

confidence: 99%

“…It has been suggested that strong interaction of nitroxoline with the free silanol groups of the column-packing material and the presence of trace metal ions were responsible for the tailing but addition of neither 8-hydroxyquinoline [2] nor Ni ion [3] to the mobile phase improved the separation of nitroxoline from structurally related compounds. For compounds with a chelating molecular structure it has been found that addition of chelating agents to the mobile phase could eliminate the effect of metal ions on chromatographic behavior, e.g.…”

Section: Optimization Of the Hplc Separationmentioning

confidence: 99%

“…The study by thinlayer chromatography has been reported [1], but interfering traces of metal ions must be removed from the silica gel by a complicated procedure. Sorel and coworkers used high-performance liquid chromatographic (HPLC) methods [2] for analysis of nitroxoline in plasma and urine, but it was shown that nitroxoline and its above mentioned structurally related impurities co-eluted.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative high-perormance liquid chromatographic analysis of nitroxoline and structurally related compounds

Kang

Wang

2003

Chromatographia

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Key WardsColumn liquid chromatography NitroxoJine 8-Hydroxyquine 8-Hyclrexy-5-nitrosoquinohne and 8-hydroxy-5,7-dinitroquinohne Plasma and urine SummaryNitroxohne (8-hydroxy-5-nitroquinohne) and the structural related compounds 8-hydroxyquine, 8-hydroxy-5,7-dinitroquinohne, and 8-hydroxy-5-nitresoquinohne have been investigated by reversed-phase high-performance liquid chromatography on C1jODS. Complete separation and symmetric peaks were obtained by use of THF-methanol-watel, 3:3:4, containing 10 mmol L 1 disodium ethylenediamine tetraacetic acid (EDTA) and 10 mmol L -1 citric acid in the water, as mobile phase. The pH and the concentration of EDTA in the mobile phase were found to be critical forehminating tailing and for full separation. The calibration plot was linear for concentrations between 3.0 and 300 Ixg mL-I; the regression coefficient was 0.99996. Assay of the nitroxohne standard showed that recovery was frorn 99.3 to ].02~ with a mean standard deviation for nitroxohne of 0.9%. The method is suitable for quality control of nitroxohne.

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Section: Optimization Of the Hplc Separationmentioning

confidence: 99%

Section: Optimization Of the Hplc Separationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative high-perormance liquid chromatographic analysis of nitroxoline and structurally related compounds

Kang

Wang

2003

Chromatographia

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“…It produces a bacteriostatic effect by the selective inhibition of the bacterial DNA synthesis. [5][6][7] Furthermore, some of these chromatographic methods 6,7 require high strength ionic buffered mobile phases which are hazardous for column efficiency and need prolonged time for column saturation and washing. Nitroxoline is active against susceptible Gram-positive and Gram-negative organisms in urinary tract infections.…”

Section: Introductionmentioning

confidence: 99%

“…Results of intra-day and inter-day assays of 1.0 Â 10 À9 M nitroxoline by the developed stripping voltammetric methods using unmodified CP and the modified CP electrodes (n ¼ 3)Table 4Assay of standard solutions of 1.0 Â 10 À8 M nitroxoline in its formulation (Nibiol® tablets) by means of the described LS-AdSV and SW-AdSV methods in comparison with RP-HPLC method6 AE 1.62 1.50 & 0.45 1.62 & 0.88 RP-HPLC 100.40 AE 1.80 (A) Calibration curve method and (B) standard addition method. The theoretical F-and t-test values at 95% condence (for n 1 ¼ 4 and n 2 ¼ 4) are 6.6 and 2.45, respectively…”

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confidence: 99%

Voltammetric analysis of nitroxoline in tablets and human serum using modified carbon paste electrodes incorporating mesoporous carbon or multiwalled carbon nanotubes

et al. 2015

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Mesoporous carbon (MC) was synthesized using a mesoporous SiO 2 template material (SBA-15) and sucrose as the carbon source. SBA-15, MC as well as commercially obtained multi-walled carbon nanotubes (MWCNTs) were characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), N 2 sorption isotherms and electrochemical impedance spectra. A carbon paste (CP) electrode modified with either MC or MWCNTs was used as a sensor for sensitive and selective determination of the nitroxoline drug. Using cyclic voltammetry, linear sweep and square-wave adsorptive stripping voltammetry, nitroxoline was found to oxidize via one developed peak at MWCNT/ CP and MC/CP electrodes compared to unmodified one. This is attributed to the stronger adsorptive character, lower charge transfer resistances and higher electrocatalytic activities of modified CP electrodes than the unmodified CP electrode. A well-defined peak and high current response were observed in a B-R universal buffer (pH 2.5) following preconcentration of nitroxoline by adsorption accumulation at À0.2 V (vs. Ag/AgCl/KCl s ). Limits of detection of 3.0 Â 10 À11 , 1.5 Â 10 À11 and 3.0 Â 10 À12 M nitroxoline, were obtained using the unmodified CP, MWCNT/CP and MC/CP electrodes, respectively. The utility of these sensors was examined for the determination of nitroxoline in its pharmaceutical dosage form (Nibiol® tablets) and human serum.

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A quality‐by‐design evaluated liquid chromatography method development and validation for the separation and quantification of nitroxoline and its impurities

Lankalapalli,

Rachel,

Chintala

et al. 2023

J of Separation Science

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A novel, isocratic, sensitive, stability‐indicating high‐performance liquid chromatography method was developed for the separation and quantification of related substances in nitroxoline (NTL). The chromatographic separation has been achieved on Inertsil ODS‐3 V, (250 × 4.6 mm, 5 μm) at 240 nm using ethylenediamine tetraacetic acid buffer and methanol in the ratio of 60:40 v/v as mobile phase. The performance of the method has been checked as per the International Conference on Harmonization guidelines for specificity, linearity, accuracy, precision, and robustness. Regression analysis showed a correlation coefficient value greater than 0.99 for NTL and its three impurities. The detection limit of impurities was in the range of 0.01% (0.05 μg/mL)–0.22% (1.1 μg/mL) indicating the sensitivity of the newly developed method. The accuracy of the method was established based on the recovery obtained between 94.7% and 104.1% for all the impurities. The percentage relative standard deviation obtained for the repeatability was less than 4.0% at the specification level for all impurities. Forced degradation was performed to establish the stability‐indicating nature of the method and to know about the degradation products, the quality of a drug substance changes with time under the influence of stress conditions. Thus, the proposed method was validated and found to be specific, sensitive, linear, accurate, precise, reproducible, and beneficial for routine usage.

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High-performance liquid chromatographic analysis of nitroxoline in plasma and urine

Cited by 12 publications

References 1 publication

Quantitative high-perormance liquid chromatographic analysis of nitroxoline and structurally related compounds

Quantitative high-perormance liquid chromatographic analysis of nitroxoline and structurally related compounds

Voltammetric analysis of nitroxoline in tablets and human serum using modified carbon paste electrodes incorporating mesoporous carbon or multiwalled carbon nanotubes

A quality‐by‐design evaluated liquid chromatography method development and validation for the separation and quantification of nitroxoline and its impurities

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